Diagnostic ability of myxovirus resistance-A in pediatric cases of viral-acute respiratory tract infections with vitamin A and zinc deficiencies
Acute respiratory tract infection (ARTI) rank among the top ten most common illnesses affecting children in Indonesia. Myxovirus resistance-A (MxA) protein, selectively induced by the activation of type I interferon in response to viral infections, has emerged as a promising biomarker to diagnose vi...
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Elsevier
2025-03-01
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| Series: | Medicine in Microecology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590097824000223 |
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| author | Dian Kesumapramudya Nurputra Amalia Setyati Nur Arfian Endy Paryanto Prawirohartono Zulvikar Syambani Ulhaq |
| author_facet | Dian Kesumapramudya Nurputra Amalia Setyati Nur Arfian Endy Paryanto Prawirohartono Zulvikar Syambani Ulhaq |
| author_sort | Dian Kesumapramudya Nurputra |
| collection | DOAJ |
| description | Acute respiratory tract infection (ARTI) rank among the top ten most common illnesses affecting children in Indonesia. Myxovirus resistance-A (MxA) protein, selectively induced by the activation of type I interferon in response to viral infections, has emerged as a promising biomarker to diagnose viral ARTI. Notably, the activation of interferons is thought to be influenced by plasma vitamin A and zinc levels. Therefore, our study aims to investigate the level of MxA expression in children with vitamin A and zinc deficiencies when experiencing viral ARTI. This cross-sectional study involved a total of 113 subjects, including 53 diagnosed with ARTI due to viral causes based on validated clinical scoring criteria, and 50 healthy controls. To determine the etiology, we conducted blood cultures and employed RT-PCR analysis on nasopharyngeal swabs. Additionally, we assessed plasma levels of vitamin A, zinc, and MxA protein expression. Our findings revealed that subjects with ARTI displayed elevated MxA expression compared to controls. Specifically, MxA expression levels in ARTI cases of viral origin were significantly higher than those in both control and bacterial origin. On closer examination, the vitamin A and zinc non-deficient group exhibited higher MxA expression levels in comparison to the deficient group. However, it is notable that the expression levels in the deficient group remained higher than those in the control group. In summary, MxA protein expression was found to be lower in children with vitamin A and zinc deficiencies when compared to those without deficiencies in cases of viral ARTI. Thus, MxA expression may serve as a diagnostic tool for distinguishing viral from bacterial ARTI, especially in populations where the prevalence of micronutrient-deficient children is high, such as in Indonesia. |
| format | Article |
| id | doaj-art-2d934346909a49809124092b75450661 |
| institution | Kabale University |
| issn | 2590-0978 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Medicine in Microecology |
| spelling | doaj-art-2d934346909a49809124092b754506612025-01-05T04:28:34ZengElsevierMedicine in Microecology2590-09782025-03-0123100119Diagnostic ability of myxovirus resistance-A in pediatric cases of viral-acute respiratory tract infections with vitamin A and zinc deficienciesDian Kesumapramudya Nurputra0Amalia Setyati1Nur Arfian2Endy Paryanto Prawirohartono3Zulvikar Syambani Ulhaq4Department of Child Health, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Paediatric Medical Group-Sardjito General Hospital, Yogyakarta, Indonesia; Corresponding author. Department of Child Health, Faculty of Medicine, Gadjah Mada University, Yogyakarta, 55281, Indonesia.Department of Child Health, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Paediatric Medical Group-Sardjito General Hospital, Yogyakarta, IndonesiaDepartment of Anatomy, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, IndonesiaDepartment of Child Health, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Paediatric Medical Group-Sardjito General Hospital, Yogyakarta, IndonesiaResearch Center for Pre-clinical and Clinical Medicine, National Research and Innovation Agency Republic of Indonesia, Cibinong, Indonesia; Corresponding author. Research Center for Pre-clinical and Clinical Medicine, National Research and Innovation Agency Republic of Indonesia, Cibinong, 16911, Indonesia.Acute respiratory tract infection (ARTI) rank among the top ten most common illnesses affecting children in Indonesia. Myxovirus resistance-A (MxA) protein, selectively induced by the activation of type I interferon in response to viral infections, has emerged as a promising biomarker to diagnose viral ARTI. Notably, the activation of interferons is thought to be influenced by plasma vitamin A and zinc levels. Therefore, our study aims to investigate the level of MxA expression in children with vitamin A and zinc deficiencies when experiencing viral ARTI. This cross-sectional study involved a total of 113 subjects, including 53 diagnosed with ARTI due to viral causes based on validated clinical scoring criteria, and 50 healthy controls. To determine the etiology, we conducted blood cultures and employed RT-PCR analysis on nasopharyngeal swabs. Additionally, we assessed plasma levels of vitamin A, zinc, and MxA protein expression. Our findings revealed that subjects with ARTI displayed elevated MxA expression compared to controls. Specifically, MxA expression levels in ARTI cases of viral origin were significantly higher than those in both control and bacterial origin. On closer examination, the vitamin A and zinc non-deficient group exhibited higher MxA expression levels in comparison to the deficient group. However, it is notable that the expression levels in the deficient group remained higher than those in the control group. In summary, MxA protein expression was found to be lower in children with vitamin A and zinc deficiencies when compared to those without deficiencies in cases of viral ARTI. Thus, MxA expression may serve as a diagnostic tool for distinguishing viral from bacterial ARTI, especially in populations where the prevalence of micronutrient-deficient children is high, such as in Indonesia.http://www.sciencedirect.com/science/article/pii/S2590097824000223Respiratory infectionDiagnosticMxA proteinMalnutritionPediatrics |
| spellingShingle | Dian Kesumapramudya Nurputra Amalia Setyati Nur Arfian Endy Paryanto Prawirohartono Zulvikar Syambani Ulhaq Diagnostic ability of myxovirus resistance-A in pediatric cases of viral-acute respiratory tract infections with vitamin A and zinc deficiencies Medicine in Microecology Respiratory infection Diagnostic MxA protein Malnutrition Pediatrics |
| title | Diagnostic ability of myxovirus resistance-A in pediatric cases of viral-acute respiratory tract infections with vitamin A and zinc deficiencies |
| title_full | Diagnostic ability of myxovirus resistance-A in pediatric cases of viral-acute respiratory tract infections with vitamin A and zinc deficiencies |
| title_fullStr | Diagnostic ability of myxovirus resistance-A in pediatric cases of viral-acute respiratory tract infections with vitamin A and zinc deficiencies |
| title_full_unstemmed | Diagnostic ability of myxovirus resistance-A in pediatric cases of viral-acute respiratory tract infections with vitamin A and zinc deficiencies |
| title_short | Diagnostic ability of myxovirus resistance-A in pediatric cases of viral-acute respiratory tract infections with vitamin A and zinc deficiencies |
| title_sort | diagnostic ability of myxovirus resistance a in pediatric cases of viral acute respiratory tract infections with vitamin a and zinc deficiencies |
| topic | Respiratory infection Diagnostic MxA protein Malnutrition Pediatrics |
| url | http://www.sciencedirect.com/science/article/pii/S2590097824000223 |
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