Investigating the Effects of Coenzyme Q10 on Human Corneal Endothelial Cells
Purpose. To investigate the effects of Coenzyme Q10 (CoQ10) treatment on immortalised human corneal endothelial cells (HCEC-12). Methods. HCEC-12 cells were cultured in different concentrations of CoQ10 (0.1%, 0.2%, 0.5%, and 1.0%) and analysed using live/dead staining assay to determine appropriate...
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| Format: | Article |
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Wiley
2021-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2021/8392572 |
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| author | Mitchell Titley Sajjad Ahmad Mohit Parekh |
| author_facet | Mitchell Titley Sajjad Ahmad Mohit Parekh |
| author_sort | Mitchell Titley |
| collection | DOAJ |
| description | Purpose. To investigate the effects of Coenzyme Q10 (CoQ10) treatment on immortalised human corneal endothelial cells (HCEC-12). Methods. HCEC-12 cells were cultured in different concentrations of CoQ10 (0.1%, 0.2%, 0.5%, and 1.0%) and analysed using live/dead staining assay to determine appropriate concentration for subsequent experiments. Cells were pretreated with CoQ10 before inducing apoptosis by ethanol (EtOH) treatment for 30 seconds which was followed by posttreatment with CoQ10. Viable, apoptotic, and dead cell proportions were analysed using Annexin V-FITC immunofluorescence staining. Mitochondrial intensity and respiratory functions were also investigated using MitoTracker staining and a Seahorse XFe24 analyser, respectively. Results were compared to a positive control for apoptosis. The experiments were carried out in triplicates. Graphpad prism software was used for statistical analysis where p<0.05 was deemed statistically significant. Results. CoQ10 treatment at 0.5% and 1% showed 92% and 30% viable cells compared with 0.1% and 0.2% that showed 96% and 94% viable cells, respectively (p=0.0562). 0.1% and 0.2% concentrations were, thus, used for subsequent experiments. Annexin V-FITC apoptotic analysis showed 2% at 0.1% and 3% at 0.2% of apoptotic cells (p=0.0824). Mitochondrial respiratory function and mitochondrial intensity increased in apoptotic cells following 0.1% CoQ10 treatment. Conclusion. 0.1% CoQ10 was found optimal for reducing apoptosis and increasing metabolic activity on human corneal endothelial cell line. These results support the need for further ex vivo studies to investigate the safety profile of CoQ10 as an antiapoptotic agent for human corneal endothelium. |
| format | Article |
| id | doaj-art-2d15ca32a004442ea60b2383bc4b0c42 |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-2d15ca32a004442ea60b2383bc4b0c422025-02-03T05:47:40ZengWileyJournal of Ophthalmology2090-004X2090-00582021-01-01202110.1155/2021/83925728392572Investigating the Effects of Coenzyme Q10 on Human Corneal Endothelial CellsMitchell Titley0Sajjad Ahmad1Mohit Parekh2Institute of Ageing and Chronic Disease, University of Liverpool, Liverpool, UKInstitute of Ophthalmology, University College London, London, UKInstitute of Ophthalmology, University College London, London, UKPurpose. To investigate the effects of Coenzyme Q10 (CoQ10) treatment on immortalised human corneal endothelial cells (HCEC-12). Methods. HCEC-12 cells were cultured in different concentrations of CoQ10 (0.1%, 0.2%, 0.5%, and 1.0%) and analysed using live/dead staining assay to determine appropriate concentration for subsequent experiments. Cells were pretreated with CoQ10 before inducing apoptosis by ethanol (EtOH) treatment for 30 seconds which was followed by posttreatment with CoQ10. Viable, apoptotic, and dead cell proportions were analysed using Annexin V-FITC immunofluorescence staining. Mitochondrial intensity and respiratory functions were also investigated using MitoTracker staining and a Seahorse XFe24 analyser, respectively. Results were compared to a positive control for apoptosis. The experiments were carried out in triplicates. Graphpad prism software was used for statistical analysis where p<0.05 was deemed statistically significant. Results. CoQ10 treatment at 0.5% and 1% showed 92% and 30% viable cells compared with 0.1% and 0.2% that showed 96% and 94% viable cells, respectively (p=0.0562). 0.1% and 0.2% concentrations were, thus, used for subsequent experiments. Annexin V-FITC apoptotic analysis showed 2% at 0.1% and 3% at 0.2% of apoptotic cells (p=0.0824). Mitochondrial respiratory function and mitochondrial intensity increased in apoptotic cells following 0.1% CoQ10 treatment. Conclusion. 0.1% CoQ10 was found optimal for reducing apoptosis and increasing metabolic activity on human corneal endothelial cell line. These results support the need for further ex vivo studies to investigate the safety profile of CoQ10 as an antiapoptotic agent for human corneal endothelium.http://dx.doi.org/10.1155/2021/8392572 |
| spellingShingle | Mitchell Titley Sajjad Ahmad Mohit Parekh Investigating the Effects of Coenzyme Q10 on Human Corneal Endothelial Cells Journal of Ophthalmology |
| title | Investigating the Effects of Coenzyme Q10 on Human Corneal Endothelial Cells |
| title_full | Investigating the Effects of Coenzyme Q10 on Human Corneal Endothelial Cells |
| title_fullStr | Investigating the Effects of Coenzyme Q10 on Human Corneal Endothelial Cells |
| title_full_unstemmed | Investigating the Effects of Coenzyme Q10 on Human Corneal Endothelial Cells |
| title_short | Investigating the Effects of Coenzyme Q10 on Human Corneal Endothelial Cells |
| title_sort | investigating the effects of coenzyme q10 on human corneal endothelial cells |
| url | http://dx.doi.org/10.1155/2021/8392572 |
| work_keys_str_mv | AT mitchelltitley investigatingtheeffectsofcoenzymeq10onhumancornealendothelialcells AT sajjadahmad investigatingtheeffectsofcoenzymeq10onhumancornealendothelialcells AT mohitparekh investigatingtheeffectsofcoenzymeq10onhumancornealendothelialcells |