A Mycovirus Representing a Novel Lineage and a Mitovirus of <i>Botrytis cinerea</i> Co-Infect a Basidiomycetous Fungus, <i>Schizophyllum commune</i>
Strain IBc-114 was isolated from a gray mold lesion and was identified as the fungus <i>Schizophyllum commune.</i> In this strain, two mycoviruses, Schizophyllum commune RNA virus 1 (ScRV1, C_AA053475.1) and Botrytis cinerea mitovirus 9 strain IBc-114 (BcMV9/IBc-114, C_AA053476.1), were...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
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| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/16/11/1767 |
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| Summary: | Strain IBc-114 was isolated from a gray mold lesion and was identified as the fungus <i>Schizophyllum commune.</i> In this strain, two mycoviruses, Schizophyllum commune RNA virus 1 (ScRV1, C_AA053475.1) and Botrytis cinerea mitovirus 9 strain IBc-114 (BcMV9/IBc-114, C_AA053476.1), were isolated and characterized. ScRV1 has flexuous filamentous particles about 20 ± 2.1 nm in diameter and 1000 ± 94.2 nm in length. The genome of ScRV1 is 7370 nt in length and contains two open reading frames (ORFs) which encode a polyprotein and a coat protein, respectively. The polyprotein has 1967 aa, including a helicase domain and an RdRp domain which has the highest identity of 28.21% with that of Entomophthora benyvirus E (EbVE). The coat protein has 241 aa which is mostly phylogenetically close to the coat proteins of <i>Alphatetraviridae</i>. Based on the phylogenetic analysis of ScRV1 and viruses selected, ScRV1 might represent a new family (temporarily named Mycobenyviridae) of the order <i>Hepelivirales</i>. The genome of BcMV9/IBc-114 that infects <i>S. commune</i> is 2729 nt in length and has only one ORF encoding an RdRp protein with 719 aa. BcMV9/IBc-114 has the highest identity of 98.61% with Botrytis cinerea mitovirus 9 (BcMV9) (MT089704). ScRV1, but not BcMV9/IBc-114, has certain effects on the host growth of <i>S. commune</i>. Furthermore, BcMV9/IBc-114 has been demonstrated to replicate in the ascomycetous fungi <i>Botrytis cinerea</i> and <i>Sclerotinia sclerotiorum</i>, and it negatively affects the growth and pathogenicity of <i>B. cinerea</i>, but it does not affect <i>S. sclerotiorum</i>. This is the first report of mycoviruses in <i>S. commune</i> and cross-phyla transmission of mitovirus in nature. |
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| ISSN: | 1999-4915 |