Biodegradation of nitro-PAHs by multi-trait PGPR strains isolated directly from rhizosphere soil

Nitrated polycyclic aromatic hydrocarbons (nitro-PAHs) are hazardous, persistent organic pollutants widely distributed globally. They significantly threaten environmental health by degrading soil, water, and air quality. Prolonged exposure to nitro-PAHs increases risks for both humans and wildlife,...

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Main Authors: Bhoirob Gogoi, Nazim Forid Islam, Hemen Sarma
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:The Microbe
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Online Access:http://www.sciencedirect.com/science/article/pii/S2950194625000317
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author Bhoirob Gogoi
Nazim Forid Islam
Hemen Sarma
author_facet Bhoirob Gogoi
Nazim Forid Islam
Hemen Sarma
author_sort Bhoirob Gogoi
collection DOAJ
description Nitrated polycyclic aromatic hydrocarbons (nitro-PAHs) are hazardous, persistent organic pollutants widely distributed globally. They significantly threaten environmental health by degrading soil, water, and air quality. Prolonged exposure to nitro-PAHs increases risks for both humans and wildlife, leading to cancer, genetic mutations, endocrine disruption, neurodegenerative disorders, and oxidative stress. This study explored the degradation of nitro-PAHs using two plant growth-promoting rhizobacterial (PGPR) strains, Bacillus cereus BG034 and Bacillus altitudinis BG05, isolated from the rhizosphere of native plants (Cyperus rotundus, Cyperus esculentus, Imperata cylindrica, and Axonopus compressus). A co-inoculum (BGC01) formed from these bacterial strains of Bacillus cereus BG034 and Bacillus altitudinis BG05, demonstrated significant capabilities for degrading nitro-PAHs. After a 72-hour incubation period, BGC01 effectively removed 76.0 % of 1-nitropyrene and 87.2 % of 2-nitrofluorene. Individually, Bacillus cereus BG034 removed 47.8 % of 1-nitropyrene and 59.9 % of 2-nitrofluorene, while Bacillus altitudinis BG05 achieved the removal abilities of 49.0 % and 59.8 %. In addition to their degradation capacity, these bacteria exhibited traits that promote plant growth. These results emphasize the potential of these bacterial strains, particularly in co-inoculum form, as effective agents for nitro-PAH degradation. This study offers an environmentally friendly and cost-effective solution for environmental remediation and highlights the potential use of these bacteria as biofertilizers for sustainable agriculture.
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spelling doaj-art-2cf05a886c3d49c1b4fbde5b83127ad02025-08-20T03:42:56ZengElsevierThe Microbe2950-19462025-03-01610026310.1016/j.microb.2025.100263Biodegradation of nitro-PAHs by multi-trait PGPR strains isolated directly from rhizosphere soilBhoirob Gogoi0Nazim Forid Islam1Hemen Sarma2Bioremediation Technology Research Group, Department of Botany, Bodoland University, Kokrajhar (BTR), Assam, IndiaInstitutional Biotech Hub, Department of Botany, N.N. Saikia College, Titabar, Assam, IndiaBioremediation Technology Research Group, Department of Botany, Bodoland University, Kokrajhar (BTR), Assam, India; Corresponding author.Nitrated polycyclic aromatic hydrocarbons (nitro-PAHs) are hazardous, persistent organic pollutants widely distributed globally. They significantly threaten environmental health by degrading soil, water, and air quality. Prolonged exposure to nitro-PAHs increases risks for both humans and wildlife, leading to cancer, genetic mutations, endocrine disruption, neurodegenerative disorders, and oxidative stress. This study explored the degradation of nitro-PAHs using two plant growth-promoting rhizobacterial (PGPR) strains, Bacillus cereus BG034 and Bacillus altitudinis BG05, isolated from the rhizosphere of native plants (Cyperus rotundus, Cyperus esculentus, Imperata cylindrica, and Axonopus compressus). A co-inoculum (BGC01) formed from these bacterial strains of Bacillus cereus BG034 and Bacillus altitudinis BG05, demonstrated significant capabilities for degrading nitro-PAHs. After a 72-hour incubation period, BGC01 effectively removed 76.0 % of 1-nitropyrene and 87.2 % of 2-nitrofluorene. Individually, Bacillus cereus BG034 removed 47.8 % of 1-nitropyrene and 59.9 % of 2-nitrofluorene, while Bacillus altitudinis BG05 achieved the removal abilities of 49.0 % and 59.8 %. In addition to their degradation capacity, these bacteria exhibited traits that promote plant growth. These results emphasize the potential of these bacterial strains, particularly in co-inoculum form, as effective agents for nitro-PAH degradation. This study offers an environmentally friendly and cost-effective solution for environmental remediation and highlights the potential use of these bacteria as biofertilizers for sustainable agriculture.http://www.sciencedirect.com/science/article/pii/S2950194625000317Hazardous pollutantsNitro-PAHs1-nitropyrene2-nitrofluoreneBioremediationCo-inoculum
spellingShingle Bhoirob Gogoi
Nazim Forid Islam
Hemen Sarma
Biodegradation of nitro-PAHs by multi-trait PGPR strains isolated directly from rhizosphere soil
The Microbe
Hazardous pollutants
Nitro-PAHs
1-nitropyrene
2-nitrofluorene
Bioremediation
Co-inoculum
title Biodegradation of nitro-PAHs by multi-trait PGPR strains isolated directly from rhizosphere soil
title_full Biodegradation of nitro-PAHs by multi-trait PGPR strains isolated directly from rhizosphere soil
title_fullStr Biodegradation of nitro-PAHs by multi-trait PGPR strains isolated directly from rhizosphere soil
title_full_unstemmed Biodegradation of nitro-PAHs by multi-trait PGPR strains isolated directly from rhizosphere soil
title_short Biodegradation of nitro-PAHs by multi-trait PGPR strains isolated directly from rhizosphere soil
title_sort biodegradation of nitro pahs by multi trait pgpr strains isolated directly from rhizosphere soil
topic Hazardous pollutants
Nitro-PAHs
1-nitropyrene
2-nitrofluorene
Bioremediation
Co-inoculum
url http://www.sciencedirect.com/science/article/pii/S2950194625000317
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