Identification and characterization of interacting proteins of transcription factor DpWRI1-like related to lipid biosynthesis from microalga Dunaliella parva
Our previous study found that Dunaliella parva WRINKLED1-like (DpWRI1-like) was a key regulatory factor of lipid biosynthesis in D. parva. DpWRI1-like gene and target genes of DpWRI1-like have been obtained in our previous study, but the interacting proteins of DpWRI1-like are unclear now, which has...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024171964 |
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| Summary: | Our previous study found that Dunaliella parva WRINKLED1-like (DpWRI1-like) was a key regulatory factor of lipid biosynthesis in D. parva. DpWRI1-like gene and target genes of DpWRI1-like have been obtained in our previous study, but the interacting proteins of DpWRI1-like are unclear now, which has limited a deep understanding of the function of DpWRI1-like. Yeast two-hybrid was widely used to identify protein-protein interaction. In this study, the interacting proteins of DpWRI1-like were obtained using yeast two-hybrid technique to further realize the role of DpWRI1-like. Three important interacting proteins have the following predicted activities: acyl-CoA-binding domain-containing protein 6 (interacting protein 1, ACBD6), duplicated carbonic anhydrase (interacting protein 2, DCA) and DNA-binding transcription factor (interacting protein 3, TF). Bimolecular fluorescence complementation assay further validated the interaction between DpWRI1-like and interacting proteins ACBD6 and DCA. The further bioinformatics analyses of interacting proteins were conducted. Protein-protein docking indicated the strong affinity between DpWRI1-like and three interacting proteins. Since interacting proteins have been found to be related to lipid biosynthesis in other organisms, this study contributes to a deeper understanding of the role of DpWRI1-like in lipid synthesis. In conclusion, this study firstly reported three interacting proteins (ACBD6, DCA and TF) of DpWRI1-like related to lipid biosynthesis, and conducted their bioinformatics analyses, which would be conducive to a deep understanding of the function of DpWRI1-like in lipid biosynthesis. |
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| ISSN: | 2405-8440 |