Subcritical water hydrolysis of Pereskia aculeate biomass to produce fermentable sugars and platform chemicals

This work aimed to conduct subcritical hydrolysis (SWH) of Pereskia aculeata Miller biomass to produce fermentable sugars and platform chemicals. For this purpose, the effect of temperature (180, 200 and 220 °C) and pressure (5, 10 and 15 MPa), at 30 and 60 min, on reducing sugars the yield (RSY), c...

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Main Authors: Fernanda Rengel dos Passos, Mônica Lady Fiorese, Edson Antonio da Silva, Gabrielle Caroline Peiter, Marcus Vinícius Tres, Lúcio Cardozo-Filho, Camila da Silva
Format: Article
Language:English
Published: Elsevier 2025-07-01
Series:Results in Chemistry
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Online Access:http://www.sciencedirect.com/science/article/pii/S2211715625004849
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Summary:This work aimed to conduct subcritical hydrolysis (SWH) of Pereskia aculeata Miller biomass to produce fermentable sugars and platform chemicals. For this purpose, the effect of temperature (180, 200 and 220 °C) and pressure (5, 10 and 15 MPa), at 30 and 60 min, on reducing sugars the yield (RSY), content of total phenolic compounds (TPC) and antioxidant potential (AP) was evaluated. The fermentable sugars and platform chemicals content of the hydrolysates were determined, in addition to which FT-IR spectra and peptides distribution by electrophoresis analysis were obtained. From the results, RSY and TPC were directly influenced by temperature, while pressure influenced only TCP. Increasing the time from 30 to 60 min provided hydrolysates with higher AP and TPC. Cellobiose was the predominant sugar in the hydrolysates and its content and platform chemical compounds were obtained at the highest reaction temperature (220 °C). Levulinic acid (41 %) and acetic acid (34 %) were the majority among the platform chemicals, while furfural and 5-HMF were detected at lower levels. From the FT-IR spectra, the effectiveness of SWH in breaking down lignocellulosic biomass was confirmed, converting them into products of interest. In addition, biopeptides of 15 and 10 kDa were identified in the hydrolysates.
ISSN:2211-7156