Gene expansion in the hawkmoth Manduca sexta drives evolution of food-associated odorant receptors
Summary: In insects, odorant receptors (ORs) are required for the detection of most olfactory cues. We investigated the function of a clade of four duplicated ORs in the hawkmoth Manduca sexta and found that these paralogs encode broadly tuned receptors with overlapping but distinct response spectra...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004224025422 |
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| author | Megha Treesa Tom Philipp Brand Sascha Bucks Jin Zhang Mario Ernesto Escobar Huezo Bill S. Hansson Sonja Bisch-Knaden |
| author_facet | Megha Treesa Tom Philipp Brand Sascha Bucks Jin Zhang Mario Ernesto Escobar Huezo Bill S. Hansson Sonja Bisch-Knaden |
| author_sort | Megha Treesa Tom |
| collection | DOAJ |
| description | Summary: In insects, odorant receptors (ORs) are required for the detection of most olfactory cues. We investigated the function of a clade of four duplicated ORs in the hawkmoth Manduca sexta and found that these paralogs encode broadly tuned receptors with overlapping but distinct response spectra. Two paralogs, which arose after divergence from a related lineage, show high sensitivity to floral esters released by a nectar-rich plant frequently visited by M. sexta. Functional imaging in mutant moths lacking one of the paralogs suggests that olfactory sensory neurons expressing this OR target a previously identified feeding-associated glomerulus in the primary olfactory center of the brain. However, only the response of this glomerulus to the single ligand unique to the now mutated OR disappeared, suggesting neuronal coexpression of the paralogs. Our results suggest a link between the studied OR expansion and enhanced detection of odors emitted by valuable nectar sources in M. sexta. |
| format | Article |
| id | doaj-art-2c11cb31f1e44f45abc0e8e3b4fb5b81 |
| institution | Kabale University |
| issn | 2589-0042 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-2c11cb31f1e44f45abc0e8e3b4fb5b812024-12-22T05:28:54ZengElsevieriScience2589-00422024-12-012712111317Gene expansion in the hawkmoth Manduca sexta drives evolution of food-associated odorant receptorsMegha Treesa Tom0Philipp Brand1Sascha Bucks2Jin Zhang3Mario Ernesto Escobar Huezo4Bill S. Hansson5Sonja Bisch-Knaden6Department of Evolutionary Neuroethology, Max-Planck Institute for Chemical Ecology, Jena, GermanyLaboratory of Neurophysiology and Behavior, The Rockefeller University, New York, NY, USADepartment of Evolutionary Neuroethology, Max-Planck Institute for Chemical Ecology, Jena, GermanyDepartment of Evolutionary Neuroethology, Max-Planck Institute for Chemical Ecology, Jena, Germany; Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing, ChinaDepartment of Evolutionary Neuroethology, Max-Planck Institute for Chemical Ecology, Jena, GermanyDepartment of Evolutionary Neuroethology, Max-Planck Institute for Chemical Ecology, Jena, GermanyDepartment of Evolutionary Neuroethology, Max-Planck Institute for Chemical Ecology, Jena, Germany; Corresponding authorSummary: In insects, odorant receptors (ORs) are required for the detection of most olfactory cues. We investigated the function of a clade of four duplicated ORs in the hawkmoth Manduca sexta and found that these paralogs encode broadly tuned receptors with overlapping but distinct response spectra. Two paralogs, which arose after divergence from a related lineage, show high sensitivity to floral esters released by a nectar-rich plant frequently visited by M. sexta. Functional imaging in mutant moths lacking one of the paralogs suggests that olfactory sensory neurons expressing this OR target a previously identified feeding-associated glomerulus in the primary olfactory center of the brain. However, only the response of this glomerulus to the single ligand unique to the now mutated OR disappeared, suggesting neuronal coexpression of the paralogs. Our results suggest a link between the studied OR expansion and enhanced detection of odors emitted by valuable nectar sources in M. sexta.http://www.sciencedirect.com/science/article/pii/S2589004224025422Sensory neuroscienceEvolutionary ecologyCell biology |
| spellingShingle | Megha Treesa Tom Philipp Brand Sascha Bucks Jin Zhang Mario Ernesto Escobar Huezo Bill S. Hansson Sonja Bisch-Knaden Gene expansion in the hawkmoth Manduca sexta drives evolution of food-associated odorant receptors iScience Sensory neuroscience Evolutionary ecology Cell biology |
| title | Gene expansion in the hawkmoth Manduca sexta drives evolution of food-associated odorant receptors |
| title_full | Gene expansion in the hawkmoth Manduca sexta drives evolution of food-associated odorant receptors |
| title_fullStr | Gene expansion in the hawkmoth Manduca sexta drives evolution of food-associated odorant receptors |
| title_full_unstemmed | Gene expansion in the hawkmoth Manduca sexta drives evolution of food-associated odorant receptors |
| title_short | Gene expansion in the hawkmoth Manduca sexta drives evolution of food-associated odorant receptors |
| title_sort | gene expansion in the hawkmoth manduca sexta drives evolution of food associated odorant receptors |
| topic | Sensory neuroscience Evolutionary ecology Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004224025422 |
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