Anti-invasion activities of heat-killed lactic acid bacteria isolates against Salmonella enterica serovar Typhimurium
Background and Objectives: The most common cause of severe foodborne salmonellosis is S. Typhimurium. Its interaction with intestinal epithelial cells is little known. Lactic acid bacteria (LAB) were recognized as a prominent probiotic gastrointestinal microbiota of humans and animals that confer h...
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Tehran University of Medical Sciences
2024-12-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/4746 |
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| author | Anis Syahirah Saifor Adzuan Sharifah Aminah Syed Mohamad Rashidah Iberahim Noor Nadia Syahira Mohd Kamal Nurliana Abd Mutalib Nur Intan Hasbullah Muneer Alsaydi Nor'aishah Hasan Low Kheng Oon Olaide Olawunmi Ajibola Rozila Alias Maimunah Mustakim Azlin Sham Rambely Emida Mohamed Mohammad Reza Pourmand |
| author_facet | Anis Syahirah Saifor Adzuan Sharifah Aminah Syed Mohamad Rashidah Iberahim Noor Nadia Syahira Mohd Kamal Nurliana Abd Mutalib Nur Intan Hasbullah Muneer Alsaydi Nor'aishah Hasan Low Kheng Oon Olaide Olawunmi Ajibola Rozila Alias Maimunah Mustakim Azlin Sham Rambely Emida Mohamed Mohammad Reza Pourmand |
| author_sort | Anis Syahirah Saifor Adzuan |
| collection | DOAJ |
| description |
Background and Objectives: The most common cause of severe foodborne salmonellosis is S. Typhimurium. Its interaction with intestinal epithelial cells is little known. Lactic acid bacteria (LAB) were recognized as a prominent probiotic gastrointestinal microbiota of humans and animals that confer health-promoting and protective effects. This study aims to determine the anti-invasion and antibacterial effects of heat-killed LAB (HK-LAB) isolates against S. Typhimurium towards human intestinal cells.
Materials and Methods: 12 HK-LAB isolates from 3 sources of origin (stingless bee, plant, and food) were tested to determine the adhesion of HK-LAB to Caco-2 cells, anti-invasion and antibacterial activities against S. Typhimurium, the adhesion and invasion pattern of S. Typhimurium on intestinal epithelial cells (Caco-2) and assessing the effect of LAB on the S. Typhimurium-host cell interaction.
Results: Tairu isolates from food have the highest adhesion rate with 19 ± 1.32/10 Caco-2 cells followed by HK-LAB R-isolate from plant 17 ± 0.70/10 Caco-2 cells, which is similar to the control (Lactobacillus casei). In the anti-invasion assay, the two HK-LAB isolates that had the strongest adherence to Caco-2 cells, Tairu-isolate inhibited at 78.1 ± 3.06% and R-isolate inhibited at 64.76 ± 9.02% compared to the positive control (63.81 ± 1.15%), which led to increased suppression of S. Typhimurium accordingly. Tairu and R isolates were tested for their antibacterial ability against S. Typhimurium. Both R and Tairu isolates displayed strong inhibition zones (27 ± 0.06 mm, 23 ± 0.06 mm) respectively.
Conclusion: These findings suggest that the anti-invasion activities of HK-LAB R and Tairu may correlate to their bactericidal effects that serve to protect the host from infection.
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| format | Article |
| id | doaj-art-2c0b86dd9801467da519d8494988a1d4 |
| institution | Kabale University |
| issn | 2008-3289 2008-4447 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj-art-2c0b86dd9801467da519d8494988a1d42025-01-06T08:38:47ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472024-12-0116610.18502/ijm.v16i6.17254Anti-invasion activities of heat-killed lactic acid bacteria isolates against Salmonella enterica serovar TyphimuriumAnis Syahirah Saifor Adzuan0Sharifah Aminah Syed Mohamad1Rashidah Iberahim2Noor Nadia Syahira Mohd Kamal3Nurliana Abd Mutalib4Nur Intan Hasbullah5Muneer Alsaydi6Nor'aishah Hasan7Low Kheng Oon8Olaide Olawunmi Ajibola9Rozila Alias10Maimunah Mustakim11Azlin Sham Rambely12Emida Mohamed13Mohammad Reza Pourmand14Microbial Metabolite Laboratory, Atta-ur-Rahman Institute for Natural Products Discovery (AuRIns), Universiti Teknologi MARA (UiTM), Puncak Alam, Selangor, Malaysia; School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), Shah Alam, Selangor, MalaysiaMicrobial Metabolite Laboratory, Atta-ur-Rahman Institute for Natural Products Discovery (AuRIns), Universiti Teknologi MARA (UiTM), Puncak Alam, Selangor, Malaysia; School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), Shah Alam, Selangor, MalaysiaSchool of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), Kuala Pilah, Negeri Sembilan, MalaysiaSchool of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), Shah Alam, Selangor, MalaysiaMicrobial Metabolite Laboratory, Atta-ur-Rahman Institute for Natural Products Discovery (AuRIns), Universiti Teknologi MARA (UiTM), Puncak Alam, Selangor, MalaysiaMicrobial Metabolite Laboratory, Atta-ur-Rahman Institute for Natural Products Discovery (AuRIns), Universiti Teknologi MARA (UiTM), Puncak Alam, Selangor, Malaysia; School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), Kuala Pilah, Negeri Sembilan, MalaysiaMicrobial Metabolite Laboratory, Atta-ur-Rahman Institute for Natural Products Discovery (AuRIns), Universiti Teknologi MARA (UiTM), Puncak Alam, Selangor, Malaysia; Department of Food Science and Technology, Faculty of Agriculture, Ibb University, Ibb, YemenSchool of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), Kuala Pilah, Negeri Sembilan, MalaysiaCell and Synthetic Biology Centre, Malaysia Genome and Vaccine Institute (MGVI), National Institutes of Biotechnology Malaysia (NIBM), Kajang, Selangor, MalaysiaFaculty of Resource Science and Technology, Universiti Malaysia Sarawak (UNIMAS), Kota Samarahan, Sarawak, MalaysiaCentre for Foundation and General Studies, Universiti Selangor, Shah Alam, Selangor, MalaysiaCentre for Medical Laboratory Technology Studies, Faculty of Health Sciences, Universiti Teknologi MARA (UiTM), Puncak Alam, Selangor, MalaysiaCentre for Medical Laboratory Technology Studies, Faculty of Health Sciences, Universiti Teknologi MARA (UiTM), Puncak Alam, Selangor, MalaysiaCentre for Medical Laboratory Technology Studies, Faculty of Health Sciences, Universiti Teknologi MARA (UiTM), Puncak Alam, Selangor, MalaysiaDepartment of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Background and Objectives: The most common cause of severe foodborne salmonellosis is S. Typhimurium. Its interaction with intestinal epithelial cells is little known. Lactic acid bacteria (LAB) were recognized as a prominent probiotic gastrointestinal microbiota of humans and animals that confer health-promoting and protective effects. This study aims to determine the anti-invasion and antibacterial effects of heat-killed LAB (HK-LAB) isolates against S. Typhimurium towards human intestinal cells. Materials and Methods: 12 HK-LAB isolates from 3 sources of origin (stingless bee, plant, and food) were tested to determine the adhesion of HK-LAB to Caco-2 cells, anti-invasion and antibacterial activities against S. Typhimurium, the adhesion and invasion pattern of S. Typhimurium on intestinal epithelial cells (Caco-2) and assessing the effect of LAB on the S. Typhimurium-host cell interaction. Results: Tairu isolates from food have the highest adhesion rate with 19 ± 1.32/10 Caco-2 cells followed by HK-LAB R-isolate from plant 17 ± 0.70/10 Caco-2 cells, which is similar to the control (Lactobacillus casei). In the anti-invasion assay, the two HK-LAB isolates that had the strongest adherence to Caco-2 cells, Tairu-isolate inhibited at 78.1 ± 3.06% and R-isolate inhibited at 64.76 ± 9.02% compared to the positive control (63.81 ± 1.15%), which led to increased suppression of S. Typhimurium accordingly. Tairu and R isolates were tested for their antibacterial ability against S. Typhimurium. Both R and Tairu isolates displayed strong inhibition zones (27 ± 0.06 mm, 23 ± 0.06 mm) respectively. Conclusion: These findings suggest that the anti-invasion activities of HK-LAB R and Tairu may correlate to their bactericidal effects that serve to protect the host from infection. https://ijm.tums.ac.ir/index.php/ijm/article/view/4746Salmonella Typhimurium;Lactic acid bacteria;Caco-2 cells;Anti-invasion |
| spellingShingle | Anis Syahirah Saifor Adzuan Sharifah Aminah Syed Mohamad Rashidah Iberahim Noor Nadia Syahira Mohd Kamal Nurliana Abd Mutalib Nur Intan Hasbullah Muneer Alsaydi Nor'aishah Hasan Low Kheng Oon Olaide Olawunmi Ajibola Rozila Alias Maimunah Mustakim Azlin Sham Rambely Emida Mohamed Mohammad Reza Pourmand Anti-invasion activities of heat-killed lactic acid bacteria isolates against Salmonella enterica serovar Typhimurium Iranian Journal of Microbiology Salmonella Typhimurium; Lactic acid bacteria; Caco-2 cells; Anti-invasion |
| title | Anti-invasion activities of heat-killed lactic acid bacteria isolates against Salmonella enterica serovar Typhimurium |
| title_full | Anti-invasion activities of heat-killed lactic acid bacteria isolates against Salmonella enterica serovar Typhimurium |
| title_fullStr | Anti-invasion activities of heat-killed lactic acid bacteria isolates against Salmonella enterica serovar Typhimurium |
| title_full_unstemmed | Anti-invasion activities of heat-killed lactic acid bacteria isolates against Salmonella enterica serovar Typhimurium |
| title_short | Anti-invasion activities of heat-killed lactic acid bacteria isolates against Salmonella enterica serovar Typhimurium |
| title_sort | anti invasion activities of heat killed lactic acid bacteria isolates against salmonella enterica serovar typhimurium |
| topic | Salmonella Typhimurium; Lactic acid bacteria; Caco-2 cells; Anti-invasion |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/4746 |
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