Identification of <i>Olea europaea CBF/DREB1</i> Family Genes in Abnormal Temperature Stress Response
<i>Olea europaea</i>, native to the Mediterranean region, has been widely cultivated for the nutritional content of its fruits and leaves. To adapt to climates beyond the Mediterranean, research on the climatic adaptation of <i>O. europaea</i> is urgently needed. Therefore, i...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
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| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/14/11/2593 |
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| Summary: | <i>Olea europaea</i>, native to the Mediterranean region, has been widely cultivated for the nutritional content of its fruits and leaves. To adapt to climates beyond the Mediterranean, research on the climatic adaptation of <i>O. europaea</i> is urgently needed. Therefore, in this study, the <i>CBF/DREB1</i> family genes in olives, which are related to cold tolerance, were genome-wide characterized. In total, four <i>OeCBFs</i> were screened; their open reading frame (ORF) were 552~684 bp in length, the encoded proteins were 20,786.60~25,235.16 Da, with 183~227 amino acids, and the theoretical isoelectric point (pI) were 4.91~9.13. The transcription of four <i>OeCBFs</i> was significantly different in olive tissues, especially <i>OeCBF2</i> and <i>OeCBF4</i>, which were substantially more highly-expressed in aboveground tissues (terminal buds, young leaves, old leaves, and stems) than in underground tissues (thick and fine roots). Exposed to cold stress, <i>OeCBF2</i> and <i>OeCBF4</i> were up-regulated more obviously in olive leaves and stems, while <i>OeCBF3</i> was induced to higher levels in the roots, implying the adaptation potential of the <i>OeCBFs</i> to low temperature stress. A comparison of the expression of the four <i>OeCBFs</i> in two varieties, Picholine (more tolerant to the other one) and Arbequina, found that the expression of <i>OeCBFs</i> was higher in most tissues of Picholine than that of Arbequina. When exposed to cold stress, the expression of the <i>OeCBFs</i> was also higher in the leaves and roots of Picholine than in Arbequina, further confirming the correlation between <i>OeCBFs</i> and cold tolerance of olive trees. This study not only deepens our understanding of the <i>CBF</i> gene family in olives, but also provides significant genetic information for olive breeding and improvement. |
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| ISSN: | 2073-4395 |