Spectroscopic study of proteins
Proteins are macromolecular compounds made up of amino acids linked by peptide bonds. They are the basic structural component of all living organisms. Their diversity has to do with their structure, and therefore with the way the amino acid residues are arranged in the molecule. Albumin shape is det...
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Śląski Uniwersytet Medyczny w Katowicach
2024-12-01
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| Series: | Annales Academiae Medicae Silesiensis |
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| Online Access: | https://annales.sum.edu.pl/Spectroscopic-study-of-proteins,193427,0,2.html |
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| author | Klaudia Kierszniok Patrycja Dolibog Barbara Pietrzyk Tomasz Pryzwan |
| author_facet | Klaudia Kierszniok Patrycja Dolibog Barbara Pietrzyk Tomasz Pryzwan |
| author_sort | Klaudia Kierszniok |
| collection | DOAJ |
| description | Proteins are macromolecular compounds made up of amino acids linked by peptide bonds. They are the basic structural component of all living organisms. Their diversity has to do with their structure, and therefore with the way the amino acid residues are arranged in the molecule. Albumin shape is determined by the sequence of amino acids that make it up. The primary amino acid in its composition is cysteine and in smaller amounts glycine, methionine and one tryptophan residue, thanks to which it exhibits fluorescent properties. For structural studies of proteins, many physical methods are used. The most commonly chosen are spectroscopic methods, which deal with the interaction between electromagnetic radiation and matter. The article provides an overview of spectroscopic methods employed in the structural studies of proteins. It also serves as a prelude to further research that aims to utilise one of the spectroscopic methods to study the structural changes of albumin after exposure to physicochemical agents. The described spectroscopic methods are characterized by different sensitivity, specificity and procedures to be performed during sample preparation for the study. In the spectroscopic study of proteins, with particular attention to changes in their structure, the method of fluorescence spectroscopy deserves attention. It is the best choice for studying structural changes in proteins that occur under the influence of physicochemical factors. Capturing these changes and relating them to the functions of proteins enriches the knowledge of the normal functioning of the body, as well as the basis of some diseases. |
| format | Article |
| id | doaj-art-2bc7dd88385c4b5baf71d430b2e0f5de |
| institution | Kabale University |
| issn | 1734-025X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Śląski Uniwersytet Medyczny w Katowicach |
| record_format | Article |
| series | Annales Academiae Medicae Silesiensis |
| spelling | doaj-art-2bc7dd88385c4b5baf71d430b2e0f5de2024-12-19T12:54:28ZengŚląski Uniwersytet Medyczny w KatowicachAnnales Academiae Medicae Silesiensis1734-025X2024-12-017832432910.18794/aams/193427Spectroscopic study of proteinsKlaudia Kierszniok0https://orcid.org/0000-0003-0418-4786Patrycja Dolibog1https://orcid.org/0000-0002-3791-0468Barbara Pietrzyk2https://orcid.org/0000-0001-8232-3054Tomasz Pryzwan3https://orcid.org/0000-0002-8966-9645Department of Medical Biophysics, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, PolandDepartment of Medical Biophysics, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, PolandDepartment of Medical Biophysics, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, PolandDepartment of Medical Biophysics, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, PolandProteins are macromolecular compounds made up of amino acids linked by peptide bonds. They are the basic structural component of all living organisms. Their diversity has to do with their structure, and therefore with the way the amino acid residues are arranged in the molecule. Albumin shape is determined by the sequence of amino acids that make it up. The primary amino acid in its composition is cysteine and in smaller amounts glycine, methionine and one tryptophan residue, thanks to which it exhibits fluorescent properties. For structural studies of proteins, many physical methods are used. The most commonly chosen are spectroscopic methods, which deal with the interaction between electromagnetic radiation and matter. The article provides an overview of spectroscopic methods employed in the structural studies of proteins. It also serves as a prelude to further research that aims to utilise one of the spectroscopic methods to study the structural changes of albumin after exposure to physicochemical agents. The described spectroscopic methods are characterized by different sensitivity, specificity and procedures to be performed during sample preparation for the study. In the spectroscopic study of proteins, with particular attention to changes in their structure, the method of fluorescence spectroscopy deserves attention. It is the best choice for studying structural changes in proteins that occur under the influence of physicochemical factors. Capturing these changes and relating them to the functions of proteins enriches the knowledge of the normal functioning of the body, as well as the basis of some diseases.https://annales.sum.edu.pl/Spectroscopic-study-of-proteins,193427,0,2.htmlalbuminfluorescence spectroscopyspectroscopic methodsproteinsprotein structure study |
| spellingShingle | Klaudia Kierszniok Patrycja Dolibog Barbara Pietrzyk Tomasz Pryzwan Spectroscopic study of proteins Annales Academiae Medicae Silesiensis albumin fluorescence spectroscopy spectroscopic methods proteins protein structure study |
| title | Spectroscopic study of proteins |
| title_full | Spectroscopic study of proteins |
| title_fullStr | Spectroscopic study of proteins |
| title_full_unstemmed | Spectroscopic study of proteins |
| title_short | Spectroscopic study of proteins |
| title_sort | spectroscopic study of proteins |
| topic | albumin fluorescence spectroscopy spectroscopic methods proteins protein structure study |
| url | https://annales.sum.edu.pl/Spectroscopic-study-of-proteins,193427,0,2.html |
| work_keys_str_mv | AT klaudiakierszniok spectroscopicstudyofproteins AT patrycjadolibog spectroscopicstudyofproteins AT barbarapietrzyk spectroscopicstudyofproteins AT tomaszpryzwan spectroscopicstudyofproteins |