Assessment of the Remineralizing Efficacy of Grape Seed Extract vs Sodium Fluoride on Surface and Subsurface Enamel Lesions: An In Vitro Study
Background: Dental caries is a prevalent oral health issue, primarily resulting from demineralization of the enamel. Remineralizing agents such as sodium fluoride (NaF) have been widely used to counteract this process. Recent studies suggest that natural extracts, such as grape seed extract (GSE), m...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-12-01
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| Series: | Journal of Pharmacy and Bioallied Sciences |
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| Online Access: | https://journals.lww.com/10.4103/jpbs.jpbs_618_24 |
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| author | Deepak Sharma Yogesh Sahu Reema Srichand Swapnali Mhatre Uttam Shetty Nikhil A. Karhale Sonu Acharya |
| author_facet | Deepak Sharma Yogesh Sahu Reema Srichand Swapnali Mhatre Uttam Shetty Nikhil A. Karhale Sonu Acharya |
| author_sort | Deepak Sharma |
| collection | DOAJ |
| description | Background:
Dental caries is a prevalent oral health issue, primarily resulting from demineralization of the enamel. Remineralizing agents such as sodium fluoride (NaF) have been widely used to counteract this process. Recent studies suggest that natural extracts, such as grape seed extract (GSE), may also have significant remineralizing properties. This study aims to compare the efficacy of GSE and NaF on surface and subsurface enamel lesions in an in vitro setting.
Materials and Methods:
In this in vitro study, 60 extracted human premolars were randomly divided into three groups of 20 each: control (no treatment), NaF treatment, and GSE treatment. Artificial enamel lesions were created on the buccal surfaces of all samples. The NaF group was treated with a 5% sodium fluoride solution, while the GSE group received a 6.5% grape seed extract solution. Treatments were applied daily for 10 days. Surface microhardness (SMH) and cross-sectional microhardness (CSMH) were measured before and after treatment to assess remineralization.
Results:
The NaF group showed a significant increase in SMH (45%) and CSMH (35%) compared to the control group. The GSE group demonstrated a similar trend with a 40% increase in SMH and a 30% increase in CSMH. Both treatments were significantly more effective than the control (P < 0.05), with no significant difference between the NaF and GSE groups (P > 0.05).
Conclusion:
Grape seed extract exhibits comparable remineralizing efficacy to sodium fluoride on both surface and subsurface enamel lesions in vitro. These findings suggest that GSE could be a viable natural alternative to fluoride for the prevention and treatment of dental caries. |
| format | Article |
| id | doaj-art-2bbc2827f985460190807915f002db46 |
| institution | Kabale University |
| issn | 0976-4879 0975-7406 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Pharmacy and Bioallied Sciences |
| spelling | doaj-art-2bbc2827f985460190807915f002db462025-01-12T14:11:03ZengWolters Kluwer Medknow PublicationsJournal of Pharmacy and Bioallied Sciences0976-48790975-74062024-12-0116Suppl 4S3203S320510.4103/jpbs.jpbs_618_24Assessment of the Remineralizing Efficacy of Grape Seed Extract vs Sodium Fluoride on Surface and Subsurface Enamel Lesions: An In Vitro StudyDeepak SharmaYogesh SahuReema SrichandSwapnali MhatreUttam ShettyNikhil A. KarhaleSonu AcharyaBackground: Dental caries is a prevalent oral health issue, primarily resulting from demineralization of the enamel. Remineralizing agents such as sodium fluoride (NaF) have been widely used to counteract this process. Recent studies suggest that natural extracts, such as grape seed extract (GSE), may also have significant remineralizing properties. This study aims to compare the efficacy of GSE and NaF on surface and subsurface enamel lesions in an in vitro setting. Materials and Methods: In this in vitro study, 60 extracted human premolars were randomly divided into three groups of 20 each: control (no treatment), NaF treatment, and GSE treatment. Artificial enamel lesions were created on the buccal surfaces of all samples. The NaF group was treated with a 5% sodium fluoride solution, while the GSE group received a 6.5% grape seed extract solution. Treatments were applied daily for 10 days. Surface microhardness (SMH) and cross-sectional microhardness (CSMH) were measured before and after treatment to assess remineralization. Results: The NaF group showed a significant increase in SMH (45%) and CSMH (35%) compared to the control group. The GSE group demonstrated a similar trend with a 40% increase in SMH and a 30% increase in CSMH. Both treatments were significantly more effective than the control (P < 0.05), with no significant difference between the NaF and GSE groups (P > 0.05). Conclusion: Grape seed extract exhibits comparable remineralizing efficacy to sodium fluoride on both surface and subsurface enamel lesions in vitro. These findings suggest that GSE could be a viable natural alternative to fluoride for the prevention and treatment of dental caries.https://journals.lww.com/10.4103/jpbs.jpbs_618_24dental cariesenamel remineralizationgrape seed extractin vitro studysodium fluoridesubsurface lesionssurface microhardness |
| spellingShingle | Deepak Sharma Yogesh Sahu Reema Srichand Swapnali Mhatre Uttam Shetty Nikhil A. Karhale Sonu Acharya Assessment of the Remineralizing Efficacy of Grape Seed Extract vs Sodium Fluoride on Surface and Subsurface Enamel Lesions: An In Vitro Study Journal of Pharmacy and Bioallied Sciences dental caries enamel remineralization grape seed extract in vitro study sodium fluoride subsurface lesions surface microhardness |
| title | Assessment of the Remineralizing Efficacy of Grape Seed Extract vs Sodium Fluoride on Surface and Subsurface Enamel Lesions: An In Vitro Study |
| title_full | Assessment of the Remineralizing Efficacy of Grape Seed Extract vs Sodium Fluoride on Surface and Subsurface Enamel Lesions: An In Vitro Study |
| title_fullStr | Assessment of the Remineralizing Efficacy of Grape Seed Extract vs Sodium Fluoride on Surface and Subsurface Enamel Lesions: An In Vitro Study |
| title_full_unstemmed | Assessment of the Remineralizing Efficacy of Grape Seed Extract vs Sodium Fluoride on Surface and Subsurface Enamel Lesions: An In Vitro Study |
| title_short | Assessment of the Remineralizing Efficacy of Grape Seed Extract vs Sodium Fluoride on Surface and Subsurface Enamel Lesions: An In Vitro Study |
| title_sort | assessment of the remineralizing efficacy of grape seed extract vs sodium fluoride on surface and subsurface enamel lesions an in vitro study |
| topic | dental caries enamel remineralization grape seed extract in vitro study sodium fluoride subsurface lesions surface microhardness |
| url | https://journals.lww.com/10.4103/jpbs.jpbs_618_24 |
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