Circulating CD3+CD8+ T Lymphocytes as Indicators of Disease Status in Patients With Early Breast Cancer
Abstract Background Identifying breast cancer markers with superior sensitivity, cost‐effectiveness, and practicality is imperative. Circulating immune cells and plasma cytokines hold promise as potential breast cancer markers. Aims To search and validate subtype of circulating immune cells and plas...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Cancer Medicine |
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| Online Access: | https://doi.org/10.1002/cam4.70547 |
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| author | Han‐Kun Chen Yi‐Ling Chen Wei‐Pang Chung Zhu‐Jun Loh Kuo‐Ting Lee Hui‐Ping Hsu |
| author_facet | Han‐Kun Chen Yi‐Ling Chen Wei‐Pang Chung Zhu‐Jun Loh Kuo‐Ting Lee Hui‐Ping Hsu |
| author_sort | Han‐Kun Chen |
| collection | DOAJ |
| description | Abstract Background Identifying breast cancer markers with superior sensitivity, cost‐effectiveness, and practicality is imperative. Circulating immune cells and plasma cytokines hold promise as potential breast cancer markers. Aims To search and validate subtype of circulating immune cells and plasma cytokines as biomarkers for breast cancer patients. Materials & Methods Using flow cytometry, we investigated circulating immune cell profiles in patients with breast cancer and healthy controls. To validate clinical observations, an orthotopic breast cancer model was established. Results We analyzed 19 healthy controls and 27 patients (22 testing group and 5 validation group) with breast cancer and revealed distinct populations, including CD3+CD4+ T lymphocytes, cytotoxic T lymphocytes (CTLs; CD3+CD8+), polymorphonuclear myeloid‐derived suppressor cells (PMN‐MDSCs), and monocytic (M)‐myeloid‐derived suppressive cells. Patients with breast cancer exhibited reduced CD3+CD4+ T lymphocyte, CD3+CD8+ CTL, and CD33+CD15− M‐MDSC levels compared with healthy controls. Diminished CD3+CD8+ CTL levels correlated with advanced cancer grade, extensive intraductal components, and positive lymphatic tumor emboli. Treatment effects included decreased T lymphocyte/PMN‐MDSC levels, contrasting with elevated circulating CD3+CD8+ cell levels posttreatment, subsequently declining upon recurrence in the validation group. Elevated plasma chemokine (C–C motif) ligand 2 (CCL2) levels distinguished patients with breast cancer from healthy controls. Our orthotopic model supported decreased circulating CD3+CD8+ CTL levels in cancer‐bearing mice, followed by a postresection increase. Discussion We found that circulating CD3+CD8+ CTL levels decreased in patients with breast cancer, increased after treatment, and decreased again upon recurrence. Conclusion Circulating CD3+CD8+ CTL emerged as promising prognostic biomarkers and therapeutic targets in breast cancer management. |
| format | Article |
| id | doaj-art-2b50bac3bfdf4ea3b61f6e026f1d5af6 |
| institution | Kabale University |
| issn | 2045-7634 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj-art-2b50bac3bfdf4ea3b61f6e026f1d5af62025-01-13T13:22:39ZengWileyCancer Medicine2045-76342025-01-01141n/an/a10.1002/cam4.70547Circulating CD3+CD8+ T Lymphocytes as Indicators of Disease Status in Patients With Early Breast CancerHan‐Kun Chen0Yi‐Ling Chen1Wei‐Pang Chung2Zhu‐Jun Loh3Kuo‐Ting Lee4Hui‐Ping Hsu5Department of Surgery Chi Mei Medical Center Tainan TaiwanDepartment of Health and Nutrition Chia Nan University of Pharmacy and Science Tainan TaiwanDepartment of Oncology, College of Medicine National Cheng Kung University Hospital, National Cheng Kung University Tainan TaiwanDepartment of Surgery, College of Medicine National Cheng Kung University Hospital, National Cheng Kung University Tainan TaiwanDepartment of Surgery, College of Medicine National Cheng Kung University Hospital, National Cheng Kung University Tainan TaiwanDepartment of Surgery, College of Medicine National Cheng Kung University Hospital, National Cheng Kung University Tainan TaiwanAbstract Background Identifying breast cancer markers with superior sensitivity, cost‐effectiveness, and practicality is imperative. Circulating immune cells and plasma cytokines hold promise as potential breast cancer markers. Aims To search and validate subtype of circulating immune cells and plasma cytokines as biomarkers for breast cancer patients. Materials & Methods Using flow cytometry, we investigated circulating immune cell profiles in patients with breast cancer and healthy controls. To validate clinical observations, an orthotopic breast cancer model was established. Results We analyzed 19 healthy controls and 27 patients (22 testing group and 5 validation group) with breast cancer and revealed distinct populations, including CD3+CD4+ T lymphocytes, cytotoxic T lymphocytes (CTLs; CD3+CD8+), polymorphonuclear myeloid‐derived suppressor cells (PMN‐MDSCs), and monocytic (M)‐myeloid‐derived suppressive cells. Patients with breast cancer exhibited reduced CD3+CD4+ T lymphocyte, CD3+CD8+ CTL, and CD33+CD15− M‐MDSC levels compared with healthy controls. Diminished CD3+CD8+ CTL levels correlated with advanced cancer grade, extensive intraductal components, and positive lymphatic tumor emboli. Treatment effects included decreased T lymphocyte/PMN‐MDSC levels, contrasting with elevated circulating CD3+CD8+ cell levels posttreatment, subsequently declining upon recurrence in the validation group. Elevated plasma chemokine (C–C motif) ligand 2 (CCL2) levels distinguished patients with breast cancer from healthy controls. Our orthotopic model supported decreased circulating CD3+CD8+ CTL levels in cancer‐bearing mice, followed by a postresection increase. Discussion We found that circulating CD3+CD8+ CTL levels decreased in patients with breast cancer, increased after treatment, and decreased again upon recurrence. Conclusion Circulating CD3+CD8+ CTL emerged as promising prognostic biomarkers and therapeutic targets in breast cancer management.https://doi.org/10.1002/cam4.70547biomarkerbreast cancerCCL2circulating immune cellscytotoxic T lymphocytes |
| spellingShingle | Han‐Kun Chen Yi‐Ling Chen Wei‐Pang Chung Zhu‐Jun Loh Kuo‐Ting Lee Hui‐Ping Hsu Circulating CD3+CD8+ T Lymphocytes as Indicators of Disease Status in Patients With Early Breast Cancer Cancer Medicine biomarker breast cancer CCL2 circulating immune cells cytotoxic T lymphocytes |
| title | Circulating CD3+CD8+ T Lymphocytes as Indicators of Disease Status in Patients With Early Breast Cancer |
| title_full | Circulating CD3+CD8+ T Lymphocytes as Indicators of Disease Status in Patients With Early Breast Cancer |
| title_fullStr | Circulating CD3+CD8+ T Lymphocytes as Indicators of Disease Status in Patients With Early Breast Cancer |
| title_full_unstemmed | Circulating CD3+CD8+ T Lymphocytes as Indicators of Disease Status in Patients With Early Breast Cancer |
| title_short | Circulating CD3+CD8+ T Lymphocytes as Indicators of Disease Status in Patients With Early Breast Cancer |
| title_sort | circulating cd3 cd8 t lymphocytes as indicators of disease status in patients with early breast cancer |
| topic | biomarker breast cancer CCL2 circulating immune cells cytotoxic T lymphocytes |
| url | https://doi.org/10.1002/cam4.70547 |
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