Overview of Clinical Relevance of Antibodies Against Oxidized Low-Density Lipoprotein (oLAb) Within Three Decades by ELISA Technology
One of the most prominent actions of oxidative stress is the attack of free radicals on poylyunsaturated fatty acids (PUFAs), initiating a chain reaction to modify these PUFAs and generate oxidized modifications on all biomolecules. In the last quarter of the 20th century, intensive research was car...
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2024-12-01
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| Series: | Antioxidants |
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| author | Willibald Wonisch Franz Tatzber Meinrad Lindschinger Andreas Falk Ulrike Resch Sabrina Mörkl Neven Zarkovic Gerhard Cvirn |
| author_facet | Willibald Wonisch Franz Tatzber Meinrad Lindschinger Andreas Falk Ulrike Resch Sabrina Mörkl Neven Zarkovic Gerhard Cvirn |
| author_sort | Willibald Wonisch |
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| description | One of the most prominent actions of oxidative stress is the attack of free radicals on poylyunsaturated fatty acids (PUFAs), initiating a chain reaction to modify these PUFAs and generate oxidized modifications on all biomolecules. In the last quarter of the 20th century, intensive research was carried out to identify antibodies against such modifications. In the mid-1990s, the first enzyme-linked immunosorbent assay (ELISA) was introduced to the market, significantly accelerating research activities and knowledge gain. During this pioneering period, the main focus was on cardiovascular diseases, cancer, diabetes, and other diseases associated with oxidative stress. Subsequently, a standard range of these antibodies against oxidized LDL (oLAb) was determined in the population. Furthermore, the impact of exhaustive physical activity and diet on oLAb titers, and the correlation between newborns and mothers after delivery, as well as nutritional intake in newborns, were evaluated. Subsequently, the harmful effects of smoking and many other areas regarding oLAb titer were published, resulting in novel approaches for prognostic and therapeutic options, in particular through studies with antioxidants, which were able to influence oLAb significantly. This review presents an overview of the research activities obtained with this ELISA over the past three decades. |
| format | Article |
| id | doaj-art-2b198bbf9be84c36b1a504cf8a644fdf |
| institution | Kabale University |
| issn | 2076-3921 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-2b198bbf9be84c36b1a504cf8a644fdf2024-12-27T14:06:58ZengMDPI AGAntioxidants2076-39212024-12-011312156010.3390/antiox13121560Overview of Clinical Relevance of Antibodies Against Oxidized Low-Density Lipoprotein (oLAb) Within Three Decades by ELISA TechnologyWillibald Wonisch0Franz Tatzber1Meinrad Lindschinger2Andreas Falk3Ulrike Resch4Sabrina Mörkl5Neven Zarkovic6Gerhard Cvirn7Otto Loewi Research Center, Division of Medicinal Chemistry, Medical University of Graz, 8010 Graz, Styria, AustriaOmnignostica Ltd., 3421 Höflein an der Donau, Lower Austria, AustriaInstitute of Nutritional and Metabolic Diseases, Outpatient Clinic Laßnitzhöhe, 8301 Laßnitzhöhe, Styria, AustriaInstitute of Nutritional and Metabolic Diseases, Outpatient Clinic Laßnitzhöhe, 8301 Laßnitzhöhe, Styria, AustriaDepartment of Vascular Biology and Thrombosis Research, Medical University of Vienna, 1090 Vienna, Vienna, AustriaDepartment of Medical Psychology, Psychosomatics and Psychotherapy, Medical University of Graz, 8036 Graz, Styria, AustriaLaboratory for Oxidative Stress, Division of Molecular Medicine, Rudjer Boskovic Institute, HR-10000 Zagreb, CroatiaOtto Loewi Research Center, Division of Medicinal Chemistry, Medical University of Graz, 8010 Graz, Styria, AustriaOne of the most prominent actions of oxidative stress is the attack of free radicals on poylyunsaturated fatty acids (PUFAs), initiating a chain reaction to modify these PUFAs and generate oxidized modifications on all biomolecules. In the last quarter of the 20th century, intensive research was carried out to identify antibodies against such modifications. In the mid-1990s, the first enzyme-linked immunosorbent assay (ELISA) was introduced to the market, significantly accelerating research activities and knowledge gain. During this pioneering period, the main focus was on cardiovascular diseases, cancer, diabetes, and other diseases associated with oxidative stress. Subsequently, a standard range of these antibodies against oxidized LDL (oLAb) was determined in the population. Furthermore, the impact of exhaustive physical activity and diet on oLAb titers, and the correlation between newborns and mothers after delivery, as well as nutritional intake in newborns, were evaluated. Subsequently, the harmful effects of smoking and many other areas regarding oLAb titer were published, resulting in novel approaches for prognostic and therapeutic options, in particular through studies with antioxidants, which were able to influence oLAb significantly. This review presents an overview of the research activities obtained with this ELISA over the past three decades.https://www.mdpi.com/2076-3921/13/12/1560antibody against oxidized LDLELISAantioxidantsoxidative stresslipid peroxidation |
| spellingShingle | Willibald Wonisch Franz Tatzber Meinrad Lindschinger Andreas Falk Ulrike Resch Sabrina Mörkl Neven Zarkovic Gerhard Cvirn Overview of Clinical Relevance of Antibodies Against Oxidized Low-Density Lipoprotein (oLAb) Within Three Decades by ELISA Technology Antioxidants antibody against oxidized LDL ELISA antioxidants oxidative stress lipid peroxidation |
| title | Overview of Clinical Relevance of Antibodies Against Oxidized Low-Density Lipoprotein (oLAb) Within Three Decades by ELISA Technology |
| title_full | Overview of Clinical Relevance of Antibodies Against Oxidized Low-Density Lipoprotein (oLAb) Within Three Decades by ELISA Technology |
| title_fullStr | Overview of Clinical Relevance of Antibodies Against Oxidized Low-Density Lipoprotein (oLAb) Within Three Decades by ELISA Technology |
| title_full_unstemmed | Overview of Clinical Relevance of Antibodies Against Oxidized Low-Density Lipoprotein (oLAb) Within Three Decades by ELISA Technology |
| title_short | Overview of Clinical Relevance of Antibodies Against Oxidized Low-Density Lipoprotein (oLAb) Within Three Decades by ELISA Technology |
| title_sort | overview of clinical relevance of antibodies against oxidized low density lipoprotein olab within three decades by elisa technology |
| topic | antibody against oxidized LDL ELISA antioxidants oxidative stress lipid peroxidation |
| url | https://www.mdpi.com/2076-3921/13/12/1560 |
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