In vitro callus induction and identification of DNA variation in Etlingera elatior using Inter-simple sequence repeat (ISSR) markers
Etlingera elatior is a promising ornamental horticultural species with various purposes such as medicinal, antibacterial agent, culinary, ornamental, and floral arrangement. The increasing demand for more variation has led to the improvements of E. elatior via tissue culture technology. Somaclonal...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Floricultura e Plantas Ornamentais
2025-01-01
|
| Series: | Ornamental Horticulture |
| Subjects: | |
| Online Access: | https://ornamentalhorticulture.com.br/rbho/article/view/2799 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841526333885120512 |
|---|---|
| author | Norsalsabila Mohd Rosli Muhamad Fahmi Yunus Tamil Chelvan Meenakshi Sundram Zarina Zainuddin Mohd Razik Midin Md Hoirul Azri Ponari |
| author_facet | Norsalsabila Mohd Rosli Muhamad Fahmi Yunus Tamil Chelvan Meenakshi Sundram Zarina Zainuddin Mohd Razik Midin Md Hoirul Azri Ponari |
| author_sort | Norsalsabila Mohd Rosli |
| collection | DOAJ |
| description |
Etlingera elatior is a promising ornamental horticultural species with various purposes such as medicinal, antibacterial agent, culinary, ornamental, and floral arrangement. The increasing demand for more variation has led to the improvements of E. elatior via tissue culture technology. Somaclonal variation helps to overcome the lack of variation of this species due to asexual propagation. The aims of this study are to induce callus and shoot and to detect genetic variations using ISSR markers. The results showed that the Murashige & Skoog (MS) basal medium supplemented with glucose and 1.5 mg L-1 6-benzylaminopurine (BAP) and 3 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D) produced significantly higher callus percentage, 50% after 20 weeks of culture. The friable calluses were then transferred to shoot induction media containing different concentrations of different BAP, 1-naphthaleneacetic acid (NAA) and thidiazuron (TDZ). After 12 weeks in shoot induction media, a root-like structure was observed in calluses masses T11 (0.1 mg L-1 NAA and 0.3 mg L-1 TDZ). Seven ISSR primers were used to evaluate the genetic variation of calluses. Seventy-two bands were generated, of which 51 bands were polymorphic with an average percentage of polymorphic bands of 72%. Jaccard’s coefficient of similarity values recorded between 0.3529 and 0.4762 exhibited the level of genetic variation among calluses. In short, the explants were affected by different concentrations of auxin and cytokinin for callus induction. ISSR markers revealed the occurrence of genetic variation during callus and shoot induction processes, suggesting a potential to generate new variants using tissue culture.
|
| format | Article |
| id | doaj-art-2ac830b0785e4fd39672715d94edaa1a |
| institution | Kabale University |
| issn | 2447-536X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Sociedade Brasileira de Floricultura e Plantas Ornamentais |
| record_format | Article |
| series | Ornamental Horticulture |
| spelling | doaj-art-2ac830b0785e4fd39672715d94edaa1a2025-01-16T23:57:50ZengSociedade Brasileira de Floricultura e Plantas OrnamentaisOrnamental Horticulture2447-536X2025-01-013110.1590/2447-536X.v31.e312799In vitro callus induction and identification of DNA variation in Etlingera elatior using Inter-simple sequence repeat (ISSR) markersNorsalsabila Mohd Rosli0Muhamad Fahmi Yunus1Tamil Chelvan Meenakshi Sundram2Zarina Zainuddin3Mohd Razik Midin4Md Hoirul Azri Ponari5International Islamic University MalaysiaInternational Islamic University MalaysiaInternational Islamic University MalaysiaInternational Islamic University MalaysiaInternational Islamic University MalaysiaInternational Islamic University Malaysia Etlingera elatior is a promising ornamental horticultural species with various purposes such as medicinal, antibacterial agent, culinary, ornamental, and floral arrangement. The increasing demand for more variation has led to the improvements of E. elatior via tissue culture technology. Somaclonal variation helps to overcome the lack of variation of this species due to asexual propagation. The aims of this study are to induce callus and shoot and to detect genetic variations using ISSR markers. The results showed that the Murashige & Skoog (MS) basal medium supplemented with glucose and 1.5 mg L-1 6-benzylaminopurine (BAP) and 3 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D) produced significantly higher callus percentage, 50% after 20 weeks of culture. The friable calluses were then transferred to shoot induction media containing different concentrations of different BAP, 1-naphthaleneacetic acid (NAA) and thidiazuron (TDZ). After 12 weeks in shoot induction media, a root-like structure was observed in calluses masses T11 (0.1 mg L-1 NAA and 0.3 mg L-1 TDZ). Seven ISSR primers were used to evaluate the genetic variation of calluses. Seventy-two bands were generated, of which 51 bands were polymorphic with an average percentage of polymorphic bands of 72%. Jaccard’s coefficient of similarity values recorded between 0.3529 and 0.4762 exhibited the level of genetic variation among calluses. In short, the explants were affected by different concentrations of auxin and cytokinin for callus induction. ISSR markers revealed the occurrence of genetic variation during callus and shoot induction processes, suggesting a potential to generate new variants using tissue culture. https://ornamentalhorticulture.com.br/rbho/article/view/2799genetic variations horticultureplant growth regulatorspolymorphismsomaclonal variation |
| spellingShingle | Norsalsabila Mohd Rosli Muhamad Fahmi Yunus Tamil Chelvan Meenakshi Sundram Zarina Zainuddin Mohd Razik Midin Md Hoirul Azri Ponari In vitro callus induction and identification of DNA variation in Etlingera elatior using Inter-simple sequence repeat (ISSR) markers Ornamental Horticulture genetic variations horticulture plant growth regulators polymorphism somaclonal variation |
| title | In vitro callus induction and identification of DNA variation in Etlingera elatior using Inter-simple sequence repeat (ISSR) markers |
| title_full | In vitro callus induction and identification of DNA variation in Etlingera elatior using Inter-simple sequence repeat (ISSR) markers |
| title_fullStr | In vitro callus induction and identification of DNA variation in Etlingera elatior using Inter-simple sequence repeat (ISSR) markers |
| title_full_unstemmed | In vitro callus induction and identification of DNA variation in Etlingera elatior using Inter-simple sequence repeat (ISSR) markers |
| title_short | In vitro callus induction and identification of DNA variation in Etlingera elatior using Inter-simple sequence repeat (ISSR) markers |
| title_sort | in vitro callus induction and identification of dna variation in etlingera elatior using inter simple sequence repeat issr markers |
| topic | genetic variations horticulture plant growth regulators polymorphism somaclonal variation |
| url | https://ornamentalhorticulture.com.br/rbho/article/view/2799 |
| work_keys_str_mv | AT norsalsabilamohdrosli invitrocallusinductionandidentificationofdnavariationinetlingeraelatiorusingintersimplesequencerepeatissrmarkers AT muhamadfahmiyunus invitrocallusinductionandidentificationofdnavariationinetlingeraelatiorusingintersimplesequencerepeatissrmarkers AT tamilchelvanmeenakshisundram invitrocallusinductionandidentificationofdnavariationinetlingeraelatiorusingintersimplesequencerepeatissrmarkers AT zarinazainuddin invitrocallusinductionandidentificationofdnavariationinetlingeraelatiorusingintersimplesequencerepeatissrmarkers AT mohdrazikmidin invitrocallusinductionandidentificationofdnavariationinetlingeraelatiorusingintersimplesequencerepeatissrmarkers AT mdhoirulazriponari invitrocallusinductionandidentificationofdnavariationinetlingeraelatiorusingintersimplesequencerepeatissrmarkers |