Developing a non-destructive method for the detection of egg quality and freshness using micro-Raman spectroscopy

Considering the pivotal role of eggs in the food industry and their nutritional significance, this study employed micro-Raman spectroscopy of eggs, examining both shells and yolks to assess the quality and freshness of eggs. Raman spectra were collected at different temperatures and time intervals t...

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Main Authors: Maryam Davari, Maryam Bahreini, Zahra Sabzevari
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Applied Food Research
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Online Access:http://www.sciencedirect.com/science/article/pii/S2772502224000659
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author Maryam Davari
Maryam Bahreini
Zahra Sabzevari
author_facet Maryam Davari
Maryam Bahreini
Zahra Sabzevari
author_sort Maryam Davari
collection DOAJ
description Considering the pivotal role of eggs in the food industry and their nutritional significance, this study employed micro-Raman spectroscopy of eggs, examining both shells and yolks to assess the quality and freshness of eggs. Raman spectra were collected at different temperatures and time intervals to investigate temperature and time effects, potentially indicating Raman peak reduction due to Maillard reaction and oxidation of proteins and lipids and carotenoid depletion, respectively. By calculating the ratio of Raman peaks, lipids, fatty acids, and choline methyl were introduced as biomarkers of temperature and time. Notable correlations were identified between Raman peaks and egg quality coefficients, including egg coefficient and peak 1002 cm−1 (protein), total weight and 1301 cm−1 (Lipids), yolk weight and 2934 and 3057 cm−1, total weight with peak 710 cm−1, and egg shape index and peak 3057 cm−1. Analysis of eggshells at different time intervals revealed Raman peak reduction during time, demonstrating Raman's effectiveness in assessing egg quality from its shell. Using the PLS-DA method, the classification of eggs at different temperatures and storage times using egg yolk Raman spectra was performed with 80 % accuracy that belongs to the validation report, predominantly influenced by carotenoid peaks, showing Raman a practical, non-destructive method for egg quality and freshness control.
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publishDate 2024-12-01
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series Applied Food Research
spelling doaj-art-2ac3b67043784b83bc0d7566ec349d4b2024-12-18T08:54:16ZengElsevierApplied Food Research2772-50222024-12-0142100453Developing a non-destructive method for the detection of egg quality and freshness using micro-Raman spectroscopyMaryam Davari0Maryam Bahreini1Zahra Sabzevari2School of Physics, Iran University of Science and Technology, Narmak, Tehran, IranCorresponding author.; School of Physics, Iran University of Science and Technology, Narmak, Tehran, IranSchool of Physics, Iran University of Science and Technology, Narmak, Tehran, IranConsidering the pivotal role of eggs in the food industry and their nutritional significance, this study employed micro-Raman spectroscopy of eggs, examining both shells and yolks to assess the quality and freshness of eggs. Raman spectra were collected at different temperatures and time intervals to investigate temperature and time effects, potentially indicating Raman peak reduction due to Maillard reaction and oxidation of proteins and lipids and carotenoid depletion, respectively. By calculating the ratio of Raman peaks, lipids, fatty acids, and choline methyl were introduced as biomarkers of temperature and time. Notable correlations were identified between Raman peaks and egg quality coefficients, including egg coefficient and peak 1002 cm−1 (protein), total weight and 1301 cm−1 (Lipids), yolk weight and 2934 and 3057 cm−1, total weight with peak 710 cm−1, and egg shape index and peak 3057 cm−1. Analysis of eggshells at different time intervals revealed Raman peak reduction during time, demonstrating Raman's effectiveness in assessing egg quality from its shell. Using the PLS-DA method, the classification of eggs at different temperatures and storage times using egg yolk Raman spectra was performed with 80 % accuracy that belongs to the validation report, predominantly influenced by carotenoid peaks, showing Raman a practical, non-destructive method for egg quality and freshness control.http://www.sciencedirect.com/science/article/pii/S2772502224000659Egg yolkEgg qualityMultivariate analysisPLS-DARaman spectroscopy
spellingShingle Maryam Davari
Maryam Bahreini
Zahra Sabzevari
Developing a non-destructive method for the detection of egg quality and freshness using micro-Raman spectroscopy
Applied Food Research
Egg yolk
Egg quality
Multivariate analysis
PLS-DA
Raman spectroscopy
title Developing a non-destructive method for the detection of egg quality and freshness using micro-Raman spectroscopy
title_full Developing a non-destructive method for the detection of egg quality and freshness using micro-Raman spectroscopy
title_fullStr Developing a non-destructive method for the detection of egg quality and freshness using micro-Raman spectroscopy
title_full_unstemmed Developing a non-destructive method for the detection of egg quality and freshness using micro-Raman spectroscopy
title_short Developing a non-destructive method for the detection of egg quality and freshness using micro-Raman spectroscopy
title_sort developing a non destructive method for the detection of egg quality and freshness using micro raman spectroscopy
topic Egg yolk
Egg quality
Multivariate analysis
PLS-DA
Raman spectroscopy
url http://www.sciencedirect.com/science/article/pii/S2772502224000659
work_keys_str_mv AT maryamdavari developinganondestructivemethodforthedetectionofeggqualityandfreshnessusingmicroramanspectroscopy
AT maryambahreini developinganondestructivemethodforthedetectionofeggqualityandfreshnessusingmicroramanspectroscopy
AT zahrasabzevari developinganondestructivemethodforthedetectionofeggqualityandfreshnessusingmicroramanspectroscopy