Age-stratification reveals age-specific intestinal microbiota signatures in juvenile idiopathic arthritis
Abstract Objective Juvenile Idiopathic Arthritis (JIA) comprises diverse chronic inflammatory conditions driven by malfunction of the immune system. The intestinal microbiota is considered a crucial environmental factor correlating with chronic inflammatory diseases, and for JIA certain alterations...
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| Language: | English |
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SpringerOpen
2024-12-01
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| Series: | Molecular and Cellular Pediatrics |
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| Online Access: | https://doi.org/10.1186/s40348-024-00186-6 |
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| author | Lisa Budzinski Toni Sempert Leonie Lietz René Maier Gi-Ung Kang Anne Sae Lim von Stuckrad Carl Christoph Goetzke Maria Roth Aayushi Shah Amro Abbas Katrin Lehman Kathleen Necke Stefanie Bartsch Ute Hoffmann Mir-Farzin Mashreghi Robert Biesen Tilmann Kallinich Hyun-Dong Chang |
| author_facet | Lisa Budzinski Toni Sempert Leonie Lietz René Maier Gi-Ung Kang Anne Sae Lim von Stuckrad Carl Christoph Goetzke Maria Roth Aayushi Shah Amro Abbas Katrin Lehman Kathleen Necke Stefanie Bartsch Ute Hoffmann Mir-Farzin Mashreghi Robert Biesen Tilmann Kallinich Hyun-Dong Chang |
| author_sort | Lisa Budzinski |
| collection | DOAJ |
| description | Abstract Objective Juvenile Idiopathic Arthritis (JIA) comprises diverse chronic inflammatory conditions driven by malfunction of the immune system. The intestinal microbiota is considered a crucial environmental factor correlating with chronic inflammatory diseases, and for JIA certain alterations in the microbiota have already been described. Methods Here, we have characterized intestinal microbiota samples from 54 JIA patients and 38 pediatric healthy controls by conventional 16S rRNA sequencing and by single-cell analysis for phenotypic features by multi-parameter microbiota flow cytometry (mMFC), which complements the population-based taxonomic profiling with the characterization of individual bacterial cells. Results We found age to be a crucial confounder in microbiota analyses of JIA patients. Age stratification revealed specific microbiota alterations neglected by the general comparison of JIA patients and pediatric controls. Conclusion Age groups presented distinct taxonomic profiles and microbiota phenotypic signatures which transitioned with age, highlighting changes in the microbiota-immune system interaction with age. |
| format | Article |
| id | doaj-art-2a94b3abc3cf4db5ac89b900873406ba |
| institution | Kabale University |
| issn | 2194-7791 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Molecular and Cellular Pediatrics |
| spelling | doaj-art-2a94b3abc3cf4db5ac89b900873406ba2024-12-15T12:09:25ZengSpringerOpenMolecular and Cellular Pediatrics2194-77912024-12-0111111310.1186/s40348-024-00186-6Age-stratification reveals age-specific intestinal microbiota signatures in juvenile idiopathic arthritisLisa Budzinski0Toni Sempert1Leonie Lietz2René Maier3Gi-Ung Kang4Anne Sae Lim von Stuckrad5Carl Christoph Goetzke6Maria Roth7Aayushi Shah8Amro Abbas9Katrin Lehman10Kathleen Necke11Stefanie Bartsch12Ute Hoffmann13Mir-Farzin Mashreghi14Robert Biesen15Tilmann Kallinich16Hyun-Dong Chang17German Rheumatology Research Center Berlin – A Leibniz InstituteGerman Rheumatology Research Center Berlin – A Leibniz InstituteGerman Rheumatology Research Center Berlin – A Leibniz InstituteGerman Rheumatology Research Center Berlin – A Leibniz InstituteGerman Rheumatology Research Center Berlin – A Leibniz InstituteDepartment of Pediatric Respiratory Medicine, Immunology and Critical Care Medicine, Charité Campus Virchow, Charité Universitätsmedizin BerlinGerman Rheumatology Research Center Berlin – A Leibniz InstituteDepartment of Rheumatology and Clinical Immunology, Charité Campus Mitte, Charité Universitätsmedizin BerlinGerman Rheumatology Research Center Berlin – A Leibniz InstituteGerman Rheumatology Research Center Berlin – A Leibniz InstituteGerman Rheumatology Research Center Berlin – A Leibniz InstituteDepartment of Pediatric Respiratory Medicine, Immunology and Critical Care Medicine, Charité Campus Virchow, Charité Universitätsmedizin BerlinDepartment of Pediatric Respiratory Medicine, Immunology and Critical Care Medicine, Charité Campus Virchow, Charité Universitätsmedizin BerlinGerman Rheumatology Research Center Berlin – A Leibniz InstituteGerman Rheumatology Research Center Berlin – A Leibniz InstituteDepartment of Rheumatology and Clinical Immunology, Charité Campus Mitte, Charité Universitätsmedizin BerlinGerman Rheumatology Research Center Berlin – A Leibniz InstituteGerman Rheumatology Research Center Berlin – A Leibniz InstituteAbstract Objective Juvenile Idiopathic Arthritis (JIA) comprises diverse chronic inflammatory conditions driven by malfunction of the immune system. The intestinal microbiota is considered a crucial environmental factor correlating with chronic inflammatory diseases, and for JIA certain alterations in the microbiota have already been described. Methods Here, we have characterized intestinal microbiota samples from 54 JIA patients and 38 pediatric healthy controls by conventional 16S rRNA sequencing and by single-cell analysis for phenotypic features by multi-parameter microbiota flow cytometry (mMFC), which complements the population-based taxonomic profiling with the characterization of individual bacterial cells. Results We found age to be a crucial confounder in microbiota analyses of JIA patients. Age stratification revealed specific microbiota alterations neglected by the general comparison of JIA patients and pediatric controls. Conclusion Age groups presented distinct taxonomic profiles and microbiota phenotypic signatures which transitioned with age, highlighting changes in the microbiota-immune system interaction with age.https://doi.org/10.1186/s40348-024-00186-6JIAMicrobiomeMicrobiota phenotypingFlow cytometry16S rRNA sequencingAge |
| spellingShingle | Lisa Budzinski Toni Sempert Leonie Lietz René Maier Gi-Ung Kang Anne Sae Lim von Stuckrad Carl Christoph Goetzke Maria Roth Aayushi Shah Amro Abbas Katrin Lehman Kathleen Necke Stefanie Bartsch Ute Hoffmann Mir-Farzin Mashreghi Robert Biesen Tilmann Kallinich Hyun-Dong Chang Age-stratification reveals age-specific intestinal microbiota signatures in juvenile idiopathic arthritis Molecular and Cellular Pediatrics JIA Microbiome Microbiota phenotyping Flow cytometry 16S rRNA sequencing Age |
| title | Age-stratification reveals age-specific intestinal microbiota signatures in juvenile idiopathic arthritis |
| title_full | Age-stratification reveals age-specific intestinal microbiota signatures in juvenile idiopathic arthritis |
| title_fullStr | Age-stratification reveals age-specific intestinal microbiota signatures in juvenile idiopathic arthritis |
| title_full_unstemmed | Age-stratification reveals age-specific intestinal microbiota signatures in juvenile idiopathic arthritis |
| title_short | Age-stratification reveals age-specific intestinal microbiota signatures in juvenile idiopathic arthritis |
| title_sort | age stratification reveals age specific intestinal microbiota signatures in juvenile idiopathic arthritis |
| topic | JIA Microbiome Microbiota phenotyping Flow cytometry 16S rRNA sequencing Age |
| url | https://doi.org/10.1186/s40348-024-00186-6 |
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