Localization of osteocalcin in bone healing treated by local application of collagen and beta-tricalcium phosphate in rats
Introduction Bone repair is a complex and multifaceted process that generally happens naturally unless complicated by situations such as substantial bone defects. The bone healing process is typically divided into three stages: inflammation, repair, and remodeling. Beta-tricalcium phosphate (β-TCP...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Russian Ilizarov Scientific Center for Restorative Traumatology and Orthopaedics
2024-12-01
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| Series: | Гений oртопедии |
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| Summary: | Introduction Bone repair is a complex and multifaceted process that generally happens naturally unless
complicated by situations such as substantial bone defects. The bone healing process is typically divided
into three stages: inflammation, repair, and remodeling. Beta-tricalcium phosphate (β-TCP) renowned
for its abundant reserves of calcium and phosphorus, easily assimilated by the body. Its exceptional
biocompatibility assists in the formation of an absorbable interlinked structure at the injury site, contributing
to the advancement of the healing process.
Purpose This study aimed to estimate the effects of a scaffold of collagen/β-tricalcium phosphate (Coll/β‑TCP)
on bone construction to evaluate its latent usage as a bone auxiliary to repair bone defects.
Material and Methods The experiment was performed on 20 adult male albino rats. Four holes were
surgically created on each animal, two in each femur; two holes were treated separately with Coll or β-TCP,
one hole with their combination. The untreated hole served as a control. Animals were scarified after twoand
four-week treatment periods (10 rats for each). Immunohistochemical analysis of bone marrow stromal
cells, osteocytes, osteoblasts and osteoclasts using polyclonal antibodies to osteocalcin was performed.
Result Immunohistochemical results discovered strong positive expression of osteocalcin in bone healing
in the group of combined treatment (β-TCP and collagen) as compared to other groups. Highly significant
differences were seen between the combination of collagen with β-TCP and the control group at both timepoints
of the experiment.
Discussion The marker osteocalcin is unique to osteoblasts, specifically to osteoblasts that are actively
forming new osteoid or remodeling bone. The obtained findings showed that mean values of osteocalcin
expression were greater in the experimental groups than in the control group.
Conclusion The combination of collagen with β-TCP showed the greatest efficacy in accelerating bone healing
and increasing osteogenic capacity due to increased osteocalcin immunoreactivity. |
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| ISSN: | 1028-4427 2542-131X |