SACCHARIFICATION OF CORNCOB USING CELLULOLYTIC BACTERIA FOR BIOETHANOL PRODUCTION

The use of cellulose degrading enzyme (cellulases) for hydrolysis of lignocellulosic material is a part of bioethanol production process.  In this experiment,  delignified corncob,  its cellulose fraction and alpha cellulose were used as substrates to produce fermentable sugar by using three loc...

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Main Authors: TITI CANDRA SUNARTI, ANJA MERYANDINI, M. EDY SOFIYANTO, NUR RICHANA
Format: Article
Language:English
Published: SEAMEO, BIOTROP 2024-01-01
Series:Biotropia: The Southeast Asian Journal of Tropical Biology
Online Access:https://journal2.biotrop.org/index.php/biotropia/article/view/80
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author TITI CANDRA SUNARTI
ANJA MERYANDINI
M. EDY SOFIYANTO
NUR RICHANA
author_facet TITI CANDRA SUNARTI
ANJA MERYANDINI
M. EDY SOFIYANTO
NUR RICHANA
author_sort TITI CANDRA SUNARTI
collection DOAJ
description The use of cellulose degrading enzyme (cellulases) for hydrolysis of lignocellulosic material is a part of bioethanol production process.  In this experiment,  delignified corncob,  its cellulose fraction and alpha cellulose were used as substrates to produce fermentable sugar by using three local isolates of celluloytic bacteria (C5-1, C4-4, C11-1 and Cmix ; mixed cultures of three isolates), and Saccharomyces cereviseae to produce ethanol. The results showed that all isolates of cellulolytic bacteria can grow on cellulose fraction better than on  delignified corncob, and alpha cellulose.  The highest hydrolytic activity produced from cellulose fraction was by isolate C4-4, which liberated 3.50 g/l of total sugar.  Ethanol can be produced by mixed culture of bacteria and yeast, but because of competitive growth, the fermentation only produced 0.39-0.47 g/l of ethanol. Keywords:  Celluloses, delignified corncob
format Article
id doaj-art-298056a6385145f38cbe72d2e850a5dd
institution Kabale University
issn 0215-6334
1907-770X
language English
publishDate 2024-01-01
publisher SEAMEO, BIOTROP
record_format Article
series Biotropia: The Southeast Asian Journal of Tropical Biology
spelling doaj-art-298056a6385145f38cbe72d2e850a5dd2025-01-16T03:47:17ZengSEAMEO, BIOTROPBiotropia: The Southeast Asian Journal of Tropical Biology0215-63341907-770X2024-01-0117210.11598/btb.2010.17.2.80SACCHARIFICATION OF CORNCOB USING CELLULOLYTIC BACTERIA FOR BIOETHANOL PRODUCTIONTITI CANDRA SUNARTI0ANJA MERYANDINI1M. EDY SOFIYANTO2NUR RICHANA31. Department of Agroindustrial Technology, Faculty of Agricultural Engineering and Technology, Bogor Agriculture University, Bogor1. Department of Agroindustrial Technology, Faculty of Agricultural Engineering and Technology, Bogor Agriculture University, Bogor1. Department of Agroindustrial Technology, Faculty of Agricultural Engineering and Technology, Bogor Agriculture University, Bogor1. Department of Agroindustrial Technology, Faculty of Agricultural Engineering and Technology, Bogor Agriculture University, Bogor The use of cellulose degrading enzyme (cellulases) for hydrolysis of lignocellulosic material is a part of bioethanol production process.  In this experiment,  delignified corncob,  its cellulose fraction and alpha cellulose were used as substrates to produce fermentable sugar by using three local isolates of celluloytic bacteria (C5-1, C4-4, C11-1 and Cmix ; mixed cultures of three isolates), and Saccharomyces cereviseae to produce ethanol. The results showed that all isolates of cellulolytic bacteria can grow on cellulose fraction better than on  delignified corncob, and alpha cellulose.  The highest hydrolytic activity produced from cellulose fraction was by isolate C4-4, which liberated 3.50 g/l of total sugar.  Ethanol can be produced by mixed culture of bacteria and yeast, but because of competitive growth, the fermentation only produced 0.39-0.47 g/l of ethanol. Keywords:  Celluloses, delignified corncob https://journal2.biotrop.org/index.php/biotropia/article/view/80
spellingShingle TITI CANDRA SUNARTI
ANJA MERYANDINI
M. EDY SOFIYANTO
NUR RICHANA
SACCHARIFICATION OF CORNCOB USING CELLULOLYTIC BACTERIA FOR BIOETHANOL PRODUCTION
Biotropia: The Southeast Asian Journal of Tropical Biology
title SACCHARIFICATION OF CORNCOB USING CELLULOLYTIC BACTERIA FOR BIOETHANOL PRODUCTION
title_full SACCHARIFICATION OF CORNCOB USING CELLULOLYTIC BACTERIA FOR BIOETHANOL PRODUCTION
title_fullStr SACCHARIFICATION OF CORNCOB USING CELLULOLYTIC BACTERIA FOR BIOETHANOL PRODUCTION
title_full_unstemmed SACCHARIFICATION OF CORNCOB USING CELLULOLYTIC BACTERIA FOR BIOETHANOL PRODUCTION
title_short SACCHARIFICATION OF CORNCOB USING CELLULOLYTIC BACTERIA FOR BIOETHANOL PRODUCTION
title_sort saccharification of corncob using cellulolytic bacteria for bioethanol production
url https://journal2.biotrop.org/index.php/biotropia/article/view/80
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AT anjameryandini saccharificationofcorncobusingcellulolyticbacteriaforbioethanolproduction
AT medysofiyanto saccharificationofcorncobusingcellulolyticbacteriaforbioethanolproduction
AT nurrichana saccharificationofcorncobusingcellulolyticbacteriaforbioethanolproduction