Personalized Microbiome Modulation to Improve Clinical Outcomes in Pediatric Inflammatory Bowel Disease: A Multi-Omics and Interventional Approach
Inflammatory bowel disease (IBD) is a complex disorder influenced by genetic, environmental, and microbial factors, with emerging evidence highlighting the gut microbiome’s role in disease pathogenesis. This study investigates the impact of microbiome-targeted interventions in pediatric IBD by integ...
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MDPI AG
2025-04-01
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| author | Adrian Goldiș Radu Dragomir Marina Adriana Mercioni Christian Goldiș Diana Sirca Ileana Enătescu Laura Olariu Oana Belei |
| author_facet | Adrian Goldiș Radu Dragomir Marina Adriana Mercioni Christian Goldiș Diana Sirca Ileana Enătescu Laura Olariu Oana Belei |
| author_sort | Adrian Goldiș |
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| description | Inflammatory bowel disease (IBD) is a complex disorder influenced by genetic, environmental, and microbial factors, with emerging evidence highlighting the gut microbiome’s role in disease pathogenesis. This study investigates the impact of microbiome-targeted interventions in pediatric IBD by integrating multi-omics analysis, including metagenomics, metabolomics, transcriptomics, and clinical biomarkers, to identify microbial dysbiosis patterns and potential therapeutic targets. A cohort of pediatric IBD patients underwent a personalized intervention involving dietary modifications, probiotic supplementation, and selective antibiotic therapy. Microbiome composition, inflammatory markers (fecal calprotectin, CRP), and disease activity scores (PCDAI/PUCAI) were assessed before and after treatment. At the 3-month follow-up, patients showed significant clinical improvement, with reduced stool frequency (<i>p</i> = 0.004) and improved stool consistency (<i>p</i> < 0.001). Symptoms such as bloating and abdominal pain decreased, while energy levels increased (<i>p</i> < 0.001). Dietary changes included higher fruit, meat, and dairy intake, and lower fast-food and sweets consumption (<i>p</i> < 0.001). Physician assessments classified 90% as “improved”, reinforcing the effectiveness of personalized microbiome interventions. Microbiome-targeted interventions (diet, probiotics, and selective antibiotics) improved pediatric IBD outcomes by reducing pathogenic bacteria and increasing short-chain fatty acid (SCFA)-producing species, lowering inflammation and symptoms. Early-life factors (cesarean birth, and formula feeding) influence IBD risk. Personalized diets enhanced microbial balance. Integrating multi-omics supports precision medicine, offering microbiome-based biomarkers and reducing immunosuppressive reliance. |
| format | Article |
| id | doaj-art-29152c898aa74b4580d08ec2c05be11f |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-29152c898aa74b4580d08ec2c05be11f2025-08-20T03:47:58ZengMDPI AGMicroorganisms2076-26072025-04-01135104710.3390/microorganisms13051047Personalized Microbiome Modulation to Improve Clinical Outcomes in Pediatric Inflammatory Bowel Disease: A Multi-Omics and Interventional ApproachAdrian Goldiș0Radu Dragomir1Marina Adriana Mercioni2Christian Goldiș3Diana Sirca4Ileana Enătescu5Laura Olariu6Oana Belei7Department of Gastroenterology and Hepatology, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timișoara, RomaniaDepartment of Obstetrics and Gynecology, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timișoara, RomaniaFaculty of Medicine, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timișoara, RomaniaFaculty of Medicine, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timișoara, RomaniaFaculty of Medicine, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timișoara, RomaniaTwelfth Department, Neonatology Clinic, “Victor Babes” University of Medicine and Pharmacy, 300041 Timișoara, RomaniaFirst Pediatric Clinic, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timișoara, RomaniaFirst Pediatric Clinic, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timișoara, RomaniaInflammatory bowel disease (IBD) is a complex disorder influenced by genetic, environmental, and microbial factors, with emerging evidence highlighting the gut microbiome’s role in disease pathogenesis. This study investigates the impact of microbiome-targeted interventions in pediatric IBD by integrating multi-omics analysis, including metagenomics, metabolomics, transcriptomics, and clinical biomarkers, to identify microbial dysbiosis patterns and potential therapeutic targets. A cohort of pediatric IBD patients underwent a personalized intervention involving dietary modifications, probiotic supplementation, and selective antibiotic therapy. Microbiome composition, inflammatory markers (fecal calprotectin, CRP), and disease activity scores (PCDAI/PUCAI) were assessed before and after treatment. At the 3-month follow-up, patients showed significant clinical improvement, with reduced stool frequency (<i>p</i> = 0.004) and improved stool consistency (<i>p</i> < 0.001). Symptoms such as bloating and abdominal pain decreased, while energy levels increased (<i>p</i> < 0.001). Dietary changes included higher fruit, meat, and dairy intake, and lower fast-food and sweets consumption (<i>p</i> < 0.001). Physician assessments classified 90% as “improved”, reinforcing the effectiveness of personalized microbiome interventions. Microbiome-targeted interventions (diet, probiotics, and selective antibiotics) improved pediatric IBD outcomes by reducing pathogenic bacteria and increasing short-chain fatty acid (SCFA)-producing species, lowering inflammation and symptoms. Early-life factors (cesarean birth, and formula feeding) influence IBD risk. Personalized diets enhanced microbial balance. Integrating multi-omics supports precision medicine, offering microbiome-based biomarkers and reducing immunosuppressive reliance.https://www.mdpi.com/2076-2607/13/5/1047pediatric inflammatory bowel disease (IBD)gut microbiomedysbiosismicrobiome-targeted therapymicrobiome modulationdietary intervention |
| spellingShingle | Adrian Goldiș Radu Dragomir Marina Adriana Mercioni Christian Goldiș Diana Sirca Ileana Enătescu Laura Olariu Oana Belei Personalized Microbiome Modulation to Improve Clinical Outcomes in Pediatric Inflammatory Bowel Disease: A Multi-Omics and Interventional Approach Microorganisms pediatric inflammatory bowel disease (IBD) gut microbiome dysbiosis microbiome-targeted therapy microbiome modulation dietary intervention |
| title | Personalized Microbiome Modulation to Improve Clinical Outcomes in Pediatric Inflammatory Bowel Disease: A Multi-Omics and Interventional Approach |
| title_full | Personalized Microbiome Modulation to Improve Clinical Outcomes in Pediatric Inflammatory Bowel Disease: A Multi-Omics and Interventional Approach |
| title_fullStr | Personalized Microbiome Modulation to Improve Clinical Outcomes in Pediatric Inflammatory Bowel Disease: A Multi-Omics and Interventional Approach |
| title_full_unstemmed | Personalized Microbiome Modulation to Improve Clinical Outcomes in Pediatric Inflammatory Bowel Disease: A Multi-Omics and Interventional Approach |
| title_short | Personalized Microbiome Modulation to Improve Clinical Outcomes in Pediatric Inflammatory Bowel Disease: A Multi-Omics and Interventional Approach |
| title_sort | personalized microbiome modulation to improve clinical outcomes in pediatric inflammatory bowel disease a multi omics and interventional approach |
| topic | pediatric inflammatory bowel disease (IBD) gut microbiome dysbiosis microbiome-targeted therapy microbiome modulation dietary intervention |
| url | https://www.mdpi.com/2076-2607/13/5/1047 |
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