Effect of the <i>Bifidobacterium animalis</i> Subsp. <i>Lactis</i>, BB-12<sup>®</sup> on <i>Cronobacter sakazakii</i> Growth in Infant Formulas with Different Acid-Buffering Capacities
The opportunistic pathogenic bacterium <i>C. sakazakii</i> poses a significant infection risk to infants through contaminated powdered infant formulae (PIFs) when proper hygiene and temperature control are neglected during reconstitution. This study aimed to investigate whether the acid-...
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2024-12-01
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| author | Anthimia Batrinou Efstathia Tsakali Vassilia J. Sinanoglou Polyvakidi Maria Eleni Katerina Pyrovolou Arhontoula Chatzilazarou Spyros J. Konteles |
| author_facet | Anthimia Batrinou Efstathia Tsakali Vassilia J. Sinanoglou Polyvakidi Maria Eleni Katerina Pyrovolou Arhontoula Chatzilazarou Spyros J. Konteles |
| author_sort | Anthimia Batrinou |
| collection | DOAJ |
| description | The opportunistic pathogenic bacterium <i>C. sakazakii</i> poses a significant infection risk to infants through contaminated powdered infant formulae (PIFs) when proper hygiene and temperature control are neglected during reconstitution. This study aimed to investigate whether the acid-buffering capacity (ABC) of commercially available PIFs enriched with the probiotic strain <i>Bifidobacterium animalis</i> subsp. lactis (BB-12<sup>®</sup>) could influence the growth of <i>C. sakazakii</i>. Two PIFs differing in their ABC were reconstituted (RIF), inoculated, and incubated in monoculture and co-culture at 22 °C and 37 °C for 24 h. After 24 h of incubation at 22 °C, regardless of the ABC type of PIF, the population of <i>C. sakazakii</i> in the monoculture was approx. 1.4 log cycles higher than the inoculum, while, in the co-culture, the <i>C. sakazakii</i> count was approx. 0.34 log cycles lower. In contrast, at 37 °C during the 24 h co-culture in the lower ABC infant formula, <i>C. sakazakii</i> was <10 CFU/mL. In all co-culture samples, the pH was significantly lower (<i>p</i> < 0.05) in the PIF with the lower ABC. An analysis of the weak organic acids at 12 and 24 h of incubation revealed that the antimicrobial activity is significantly affected by the final pH value, the type of the weak organic acids, and their ionic–non-ionic ratio, which is formed through the common ion effect. |
| format | Article |
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| institution | Kabale University |
| issn | 2076-3417 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj-art-290b5c9618f645e6b9502a87f94fa5032025-01-10T13:14:31ZengMDPI AGApplied Sciences2076-34172024-12-0115112410.3390/app15010124Effect of the <i>Bifidobacterium animalis</i> Subsp. <i>Lactis</i>, BB-12<sup>®</sup> on <i>Cronobacter sakazakii</i> Growth in Infant Formulas with Different Acid-Buffering CapacitiesAnthimia Batrinou0Efstathia Tsakali1Vassilia J. Sinanoglou2Polyvakidi Maria Eleni3Katerina Pyrovolou4Arhontoula Chatzilazarou5Spyros J. Konteles6Department of Food Science and Technology, University of West Attica, Ag. Spyridonos 28, 12243 Egaleo, GreeceDepartment of Food Science and Technology, University of West Attica, Ag. Spyridonos 28, 12243 Egaleo, GreeceDepartment of Food Science and Technology, University of West Attica, Ag. Spyridonos 28, 12243 Egaleo, GreeceDepartment of Food Science and Technology, University of West Attica, Ag. Spyridonos 28, 12243 Egaleo, GreeceDepartment of Food Science and Technology, University of West Attica, Ag. Spyridonos 28, 12243 Egaleo, GreeceDepartment of Wine, Vine and Beverage Sciences, University of West Attica, Ag. Spyridonos 28, 12243 Egaleo, GreeceDepartment of Food Science and Technology, University of West Attica, Ag. Spyridonos 28, 12243 Egaleo, GreeceThe opportunistic pathogenic bacterium <i>C. sakazakii</i> poses a significant infection risk to infants through contaminated powdered infant formulae (PIFs) when proper hygiene and temperature control are neglected during reconstitution. This study aimed to investigate whether the acid-buffering capacity (ABC) of commercially available PIFs enriched with the probiotic strain <i>Bifidobacterium animalis</i> subsp. lactis (BB-12<sup>®</sup>) could influence the growth of <i>C. sakazakii</i>. Two PIFs differing in their ABC were reconstituted (RIF), inoculated, and incubated in monoculture and co-culture at 22 °C and 37 °C for 24 h. After 24 h of incubation at 22 °C, regardless of the ABC type of PIF, the population of <i>C. sakazakii</i> in the monoculture was approx. 1.4 log cycles higher than the inoculum, while, in the co-culture, the <i>C. sakazakii</i> count was approx. 0.34 log cycles lower. In contrast, at 37 °C during the 24 h co-culture in the lower ABC infant formula, <i>C. sakazakii</i> was <10 CFU/mL. In all co-culture samples, the pH was significantly lower (<i>p</i> < 0.05) in the PIF with the lower ABC. An analysis of the weak organic acids at 12 and 24 h of incubation revealed that the antimicrobial activity is significantly affected by the final pH value, the type of the weak organic acids, and their ionic–non-ionic ratio, which is formed through the common ion effect.https://www.mdpi.com/2076-3417/15/1/124powdered infant formula (PIF)probiotic bacterium<i>Cronobacter sakazakii</i>acid-buffering capacitymicrobiological analysishigh-performance liquid chromatographic (HPLC) analysis |
| spellingShingle | Anthimia Batrinou Efstathia Tsakali Vassilia J. Sinanoglou Polyvakidi Maria Eleni Katerina Pyrovolou Arhontoula Chatzilazarou Spyros J. Konteles Effect of the <i>Bifidobacterium animalis</i> Subsp. <i>Lactis</i>, BB-12<sup>®</sup> on <i>Cronobacter sakazakii</i> Growth in Infant Formulas with Different Acid-Buffering Capacities Applied Sciences powdered infant formula (PIF) probiotic bacterium <i>Cronobacter sakazakii</i> acid-buffering capacity microbiological analysis high-performance liquid chromatographic (HPLC) analysis |
| title | Effect of the <i>Bifidobacterium animalis</i> Subsp. <i>Lactis</i>, BB-12<sup>®</sup> on <i>Cronobacter sakazakii</i> Growth in Infant Formulas with Different Acid-Buffering Capacities |
| title_full | Effect of the <i>Bifidobacterium animalis</i> Subsp. <i>Lactis</i>, BB-12<sup>®</sup> on <i>Cronobacter sakazakii</i> Growth in Infant Formulas with Different Acid-Buffering Capacities |
| title_fullStr | Effect of the <i>Bifidobacterium animalis</i> Subsp. <i>Lactis</i>, BB-12<sup>®</sup> on <i>Cronobacter sakazakii</i> Growth in Infant Formulas with Different Acid-Buffering Capacities |
| title_full_unstemmed | Effect of the <i>Bifidobacterium animalis</i> Subsp. <i>Lactis</i>, BB-12<sup>®</sup> on <i>Cronobacter sakazakii</i> Growth in Infant Formulas with Different Acid-Buffering Capacities |
| title_short | Effect of the <i>Bifidobacterium animalis</i> Subsp. <i>Lactis</i>, BB-12<sup>®</sup> on <i>Cronobacter sakazakii</i> Growth in Infant Formulas with Different Acid-Buffering Capacities |
| title_sort | effect of the i bifidobacterium animalis i subsp i lactis i bb 12 sup r sup on i cronobacter sakazakii i growth in infant formulas with different acid buffering capacities |
| topic | powdered infant formula (PIF) probiotic bacterium <i>Cronobacter sakazakii</i> acid-buffering capacity microbiological analysis high-performance liquid chromatographic (HPLC) analysis |
| url | https://www.mdpi.com/2076-3417/15/1/124 |
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