Effect of the <i>Bifidobacterium animalis</i> Subsp. <i>Lactis</i>, BB-12<sup>®</sup> on <i>Cronobacter sakazakii</i> Growth in Infant Formulas with Different Acid-Buffering Capacities

Effect of the <i>Bifidobacterium animalis</i> Subsp. <i>Lactis</i>, BB-12<sup>®</sup> on <i>Cronobacter sakazakii</i> Growth in Infant Formulas with Different Acid-Buffering Capacities

The opportunistic pathogenic bacterium <i>C. sakazakii</i> poses a significant infection risk to infants through contaminated powdered infant formulae (PIFs) when proper hygiene and temperature control are neglected during reconstitution. This study aimed to investigate whether the acid-...

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Main Authors: Anthimia Batrinou, Efstathia Tsakali, Vassilia J. Sinanoglou, Polyvakidi Maria Eleni, Katerina Pyrovolou, Arhontoula Chatzilazarou, Spyros J. Konteles
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Applied Sciences
Subjects:
powdered infant formula (PIF)
probiotic bacterium
<i>Cronobacter sakazakii</i>
acid-buffering capacity
microbiological analysis
high-performance liquid chromatographic (HPLC) analysis
Online Access:https://www.mdpi.com/2076-3417/15/1/124
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Summary:The opportunistic pathogenic bacterium <i>C. sakazakii</i> poses a significant infection risk to infants through contaminated powdered infant formulae (PIFs) when proper hygiene and temperature control are neglected during reconstitution. This study aimed to investigate whether the acid-buffering capacity (ABC) of commercially available PIFs enriched with the probiotic strain <i>Bifidobacterium animalis</i> subsp. lactis (BB-12<sup>®</sup>) could influence the growth of <i>C. sakazakii</i>. Two PIFs differing in their ABC were reconstituted (RIF), inoculated, and incubated in monoculture and co-culture at 22 °C and 37 °C for 24 h. After 24 h of incubation at 22 °C, regardless of the ABC type of PIF, the population of <i>C. sakazakii</i> in the monoculture was approx. 1.4 log cycles higher than the inoculum, while, in the co-culture, the <i>C. sakazakii</i> count was approx. 0.34 log cycles lower. In contrast, at 37 °C during the 24 h co-culture in the lower ABC infant formula, <i>C. sakazakii</i> was <10 CFU/mL. In all co-culture samples, the pH was significantly lower (<i>p</i> < 0.05) in the PIF with the lower ABC. An analysis of the weak organic acids at 12 and 24 h of incubation revealed that the antimicrobial activity is significantly affected by the final pH value, the type of the weak organic acids, and their ionic–non-ionic ratio, which is formed through the common ion effect.
ISSN:2076-3417

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