Baseline Raman Spectral Fingerprints of Zebrafish Embryos and Larvae
As a highly sensitive vibrational technique, Raman spectroscopy (RS) can provide valuable chemical and molecular data useful to characterise animal cell types, tissues and organs. As a label-free, rapid detection method, RS has been considered a valuable asset in forensics, biology and medicine. The...
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MDPI AG
2024-11-01
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| author | Isabel Oliveira Abreu Cláudia Teixeira Rui Vilarinho A. Cristina S. Rocha Joaquim Agostinho Moreira Luís Oliva-Teles Laura Guimarães António Paulo Carvalho |
| author_facet | Isabel Oliveira Abreu Cláudia Teixeira Rui Vilarinho A. Cristina S. Rocha Joaquim Agostinho Moreira Luís Oliva-Teles Laura Guimarães António Paulo Carvalho |
| author_sort | Isabel Oliveira Abreu |
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| description | As a highly sensitive vibrational technique, Raman spectroscopy (RS) can provide valuable chemical and molecular data useful to characterise animal cell types, tissues and organs. As a label-free, rapid detection method, RS has been considered a valuable asset in forensics, biology and medicine. The technique has been applied to zebrafish for various purposes, including physiological, biochemical or bioaccumulation analyses. The available data point out its potential for the early diagnosis of detrimental effects elicited by toxicant exposure. Nevertheless, no baseline spectra are available for zebrafish embryos and larvae that could allow for suitable planning of toxicological assessments, comparison with toxicant-elicited spectra or mechanistic understanding of biochemical and physiological responses to the exposures. With this in mind, this work carried out a baseline characterisation of Raman spectra of zebrafish embryos and larvae throughout early development. Raman spectra were recorded from the iris, forebrain, melanocytes, heart, muscle and swim bladder between 24 and 168 h post-fertilisation. A chemometrics approach, based on partial least-squares discriminant analysis (PLS-DA), was used to obtain a Raman characterisation of each tissue or organ. In total, 117 Raman bands were identified, of which 24 were well represented and, thus, retained in the data analysed. Only three bands were found to be common to all organs and tissues. The PLS-DA provided a tentative Raman spectral fingerprint typical of each tissue or organ, reflecting the ongoing developmental dynamics. The bands showed frequencies previously assigned to collagen, cholesterol, various essential amino acids, carbohydrates and nucleic acids. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Biosensors |
| spelling | doaj-art-28fc05f13c584c989d1e056c195ce9b32024-11-26T17:54:37ZengMDPI AGBiosensors2079-63742024-11-01141153810.3390/bios14110538Baseline Raman Spectral Fingerprints of Zebrafish Embryos and LarvaeIsabel Oliveira Abreu0Cláudia Teixeira1Rui Vilarinho2A. Cristina S. Rocha3Joaquim Agostinho Moreira4Luís Oliva-Teles5Laura Guimarães6António Paulo Carvalho7CIIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos, s/n, 4450-208 Matosinhos, PortugalCIIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos, s/n, 4450-208 Matosinhos, PortugalIFIMUP—Institute of Physics for Advanced Materials, Nanotechnology and Photonics, Faculty of Sciences of the University of Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, PortugalMARE—Marine and Environmental Sciences Centre/ARNET—Aquatic Research Network, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456 Coimbra, PortugalIFIMUP—Institute of Physics for Advanced Materials, Nanotechnology and Photonics, Faculty of Sciences of the University of Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, PortugalCIIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos, s/n, 4450-208 Matosinhos, PortugalCIIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos, s/n, 4450-208 Matosinhos, PortugalCIIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos, s/n, 4450-208 Matosinhos, PortugalAs a highly sensitive vibrational technique, Raman spectroscopy (RS) can provide valuable chemical and molecular data useful to characterise animal cell types, tissues and organs. As a label-free, rapid detection method, RS has been considered a valuable asset in forensics, biology and medicine. The technique has been applied to zebrafish for various purposes, including physiological, biochemical or bioaccumulation analyses. The available data point out its potential for the early diagnosis of detrimental effects elicited by toxicant exposure. Nevertheless, no baseline spectra are available for zebrafish embryos and larvae that could allow for suitable planning of toxicological assessments, comparison with toxicant-elicited spectra or mechanistic understanding of biochemical and physiological responses to the exposures. With this in mind, this work carried out a baseline characterisation of Raman spectra of zebrafish embryos and larvae throughout early development. Raman spectra were recorded from the iris, forebrain, melanocytes, heart, muscle and swim bladder between 24 and 168 h post-fertilisation. A chemometrics approach, based on partial least-squares discriminant analysis (PLS-DA), was used to obtain a Raman characterisation of each tissue or organ. In total, 117 Raman bands were identified, of which 24 were well represented and, thus, retained in the data analysed. Only three bands were found to be common to all organs and tissues. The PLS-DA provided a tentative Raman spectral fingerprint typical of each tissue or organ, reflecting the ongoing developmental dynamics. The bands showed frequencies previously assigned to collagen, cholesterol, various essential amino acids, carbohydrates and nucleic acids.https://www.mdpi.com/2079-6374/14/11/538zebrafish organsRaman bandsdevelopmental dynamicsamino acidslipids |
| spellingShingle | Isabel Oliveira Abreu Cláudia Teixeira Rui Vilarinho A. Cristina S. Rocha Joaquim Agostinho Moreira Luís Oliva-Teles Laura Guimarães António Paulo Carvalho Baseline Raman Spectral Fingerprints of Zebrafish Embryos and Larvae Biosensors zebrafish organs Raman bands developmental dynamics amino acids lipids |
| title | Baseline Raman Spectral Fingerprints of Zebrafish Embryos and Larvae |
| title_full | Baseline Raman Spectral Fingerprints of Zebrafish Embryos and Larvae |
| title_fullStr | Baseline Raman Spectral Fingerprints of Zebrafish Embryos and Larvae |
| title_full_unstemmed | Baseline Raman Spectral Fingerprints of Zebrafish Embryos and Larvae |
| title_short | Baseline Raman Spectral Fingerprints of Zebrafish Embryos and Larvae |
| title_sort | baseline raman spectral fingerprints of zebrafish embryos and larvae |
| topic | zebrafish organs Raman bands developmental dynamics amino acids lipids |
| url | https://www.mdpi.com/2079-6374/14/11/538 |
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