Lean and eco-efficient method for evaluating the potency of tinidazole in tablets
Tinidazole (TIN), an amoebicide and giardicide, does not present microbiological methods in official compendia for evaluating the potency of final products. The objective of this work is to develop and validate an effective, lean and eco-efficient microbiological turbidimetric method by National En...
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| Language: | English |
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Universidade Federal do Rio Grande do Sul
2024-12-01
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| Series: | Drug Analytical Research |
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| Online Access: | https://seer.ufrgs.br/rita/citati/index.php/dar/article/view/143057 |
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| author | Pollyanna Barbara Henrique Ieda Sapateiro Torres Ana Kogawa |
| author_facet | Pollyanna Barbara Henrique Ieda Sapateiro Torres Ana Kogawa |
| author_sort | Pollyanna Barbara Henrique |
| collection | DOAJ |
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Tinidazole (TIN), an amoebicide and giardicide, does not present microbiological methods in official compendia for evaluating the potency of final products. The objective of this work is to develop and validate an effective, lean and eco-efficient microbiological turbidimetric method by National Environmental Method Index (NEMI) and Eco-Scale Assessment (ESA) to evaluate the potency of TIN-based tablets. The microbiological method was performed using Escherichia coli ATCC 25922 at 1 % in BHI broth, TIN solution in purified water and ethanol (90:10, v/v) at concentrations of 40, 60 and 90 μg mL-1, shaker at 80 rpm, 4 hours of incubation, quartz cuvette and 530 nm. The method was linear from 40 to 90 μg mL-1 with a correlation coefficient of 0.9991, selective, precise (RSD < 4 %), accurate with 99.65 % recovery and robust to changes in culture medium volume, culture medium brand, inoculum percentage and shaker rotation speed. The potency of TIN tablets was 98.50 %. The greenness of the method was evaluated and NEMI presented 3 green quadrants and the ESA score was 93, showing an excellent green analysis option. This work presents a lean and eco-efficient proposal for evaluating the potency of TIN tablets for chemical-pharmaceutical laboratories around the world.
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| format | Article |
| id | doaj-art-28c7635d3ad64fdfa7ac212fb565ac51 |
| institution | Kabale University |
| issn | 2527-2616 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Universidade Federal do Rio Grande do Sul |
| record_format | Article |
| series | Drug Analytical Research |
| spelling | doaj-art-28c7635d3ad64fdfa7ac212fb565ac512025-01-07T18:21:26ZengUniversidade Federal do Rio Grande do SulDrug Analytical Research2527-26162024-12-018210.22456/2527-2616.143057Lean and eco-efficient method for evaluating the potency of tinidazole in tabletsPollyanna Barbara HenriqueIeda Sapateiro TorresAna Kogawa0Universidade Federal de Goiás Tinidazole (TIN), an amoebicide and giardicide, does not present microbiological methods in official compendia for evaluating the potency of final products. The objective of this work is to develop and validate an effective, lean and eco-efficient microbiological turbidimetric method by National Environmental Method Index (NEMI) and Eco-Scale Assessment (ESA) to evaluate the potency of TIN-based tablets. The microbiological method was performed using Escherichia coli ATCC 25922 at 1 % in BHI broth, TIN solution in purified water and ethanol (90:10, v/v) at concentrations of 40, 60 and 90 μg mL-1, shaker at 80 rpm, 4 hours of incubation, quartz cuvette and 530 nm. The method was linear from 40 to 90 μg mL-1 with a correlation coefficient of 0.9991, selective, precise (RSD < 4 %), accurate with 99.65 % recovery and robust to changes in culture medium volume, culture medium brand, inoculum percentage and shaker rotation speed. The potency of TIN tablets was 98.50 %. The greenness of the method was evaluated and NEMI presented 3 green quadrants and the ESA score was 93, showing an excellent green analysis option. This work presents a lean and eco-efficient proposal for evaluating the potency of TIN tablets for chemical-pharmaceutical laboratories around the world. https://seer.ufrgs.br/rita/citati/index.php/dar/article/view/143057microbiological methodantimicrobial potencytinidazoletabletsGreen Analytical Chemistry |
| spellingShingle | Pollyanna Barbara Henrique Ieda Sapateiro Torres Ana Kogawa Lean and eco-efficient method for evaluating the potency of tinidazole in tablets Drug Analytical Research microbiological method antimicrobial potency tinidazole tablets Green Analytical Chemistry |
| title | Lean and eco-efficient method for evaluating the potency of tinidazole in tablets |
| title_full | Lean and eco-efficient method for evaluating the potency of tinidazole in tablets |
| title_fullStr | Lean and eco-efficient method for evaluating the potency of tinidazole in tablets |
| title_full_unstemmed | Lean and eco-efficient method for evaluating the potency of tinidazole in tablets |
| title_short | Lean and eco-efficient method for evaluating the potency of tinidazole in tablets |
| title_sort | lean and eco efficient method for evaluating the potency of tinidazole in tablets |
| topic | microbiological method antimicrobial potency tinidazole tablets Green Analytical Chemistry |
| url | https://seer.ufrgs.br/rita/citati/index.php/dar/article/view/143057 |
| work_keys_str_mv | AT pollyannabarbarahenrique leanandecoefficientmethodforevaluatingthepotencyoftinidazoleintablets AT iedasapateirotorres leanandecoefficientmethodforevaluatingthepotencyoftinidazoleintablets AT anakogawa leanandecoefficientmethodforevaluatingthepotencyoftinidazoleintablets |