Differential editing efficiencies in cereal crops: a comparative analysis of tRNA and ribozyme multiplexed guide delivery

Differential editing efficiencies in cereal crops: a comparative analysis of tRNA and ribozyme multiplexed guide delivery

Cereal transformation and gene editing can be a complex and costly undertaking. It is therefore important to validate and understand the performance of the components to achieve high rates of transformation and gene editing. Here, we have made a direct comparison of different CRISPR/Cas9 guide syste...

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Bibliographic Details
Main Authors: Matthew J. Milner, Manisha Sharma, Ruth E. Bates, Michelle Whiting, Melanie S. Craze, Peter Miller, Jack Brooks, Allan Kouidri, Emma J. Wallington
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-12-01
Series:Frontiers in Plant Science
Subjects:
CRISPR
tRNA
ribozyme
rice
wheat
barley
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2024.1426184/full
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