Integrated membranes system for water application in microbiology/molecular biology
Abstract An integrated membrane filtration system was developed to make water purity suitable for microbiology/molecular biology research. Water samples were collected from outlets in different buildings of the National Research Center and analyzed for their composition before filtration. An integra...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2024-10-01
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| Series: | Applied Water Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/s13201-024-02294-0 |
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| _version_ | 1846164957910007808 |
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| author | Heba Abdallah Dina Nadeem Abd-Elshafy Marwa Shalaby Kareem Awad Rola Nadeem Ahmed Shaban Mahmoud Mohamed Bahgat |
| author_facet | Heba Abdallah Dina Nadeem Abd-Elshafy Marwa Shalaby Kareem Awad Rola Nadeem Ahmed Shaban Mahmoud Mohamed Bahgat |
| author_sort | Heba Abdallah |
| collection | DOAJ |
| description | Abstract An integrated membrane filtration system was developed to make water purity suitable for microbiology/molecular biology research. Water samples were collected from outlets in different buildings of the National Research Center and analyzed for their composition before filtration. An integrated membrane system was developed based on mathematical modeling. Flat sheet membranes were produced, including microfiltration, ultrafiltration, and nanofiltration membranes. The flat sheet membranes were converted to a spiral wound module filter to simulate the local market filters and applied in the integrated membrane system that was designed and installed. The produced water was analyzed and compared to molecular-grade water used in different molecular biology/microbiology applications. Both PCR, agarose gel electrophoresis, bacterial liquid cultures, and viral propagation indicated that treated water using the herein-developed system exhibited comparable performance to the molecular grade water provided with imported reagent kits. So, this research can offer a promising solution for producing high-quality water suitable for sensitive laboratory applications. |
| format | Article |
| id | doaj-art-2841422a69e0444daa96d2fe9c32b791 |
| institution | Kabale University |
| issn | 2190-5487 2190-5495 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Applied Water Science |
| spelling | doaj-art-2841422a69e0444daa96d2fe9c32b7912024-11-17T12:40:38ZengSpringerOpenApplied Water Science2190-54872190-54952024-10-01141111310.1007/s13201-024-02294-0Integrated membranes system for water application in microbiology/molecular biologyHeba Abdallah0Dina Nadeem Abd-Elshafy1Marwa Shalaby2Kareem Awad3Rola Nadeem4Ahmed Shaban5Mahmoud Mohamed Bahgat6Chemical Engineering and Pilot Plant Department, Engineering and Renewable Energy Research Institute, National Research CentreEnvironmental Virology Laboratory, Department of Water Pollution Research, Environmental and Climate Change Research Institute, National Research CentreChemical Engineering and Pilot Plant Department, Engineering and Renewable Energy Research Institute, National Research CentreResearch Group Immune- and Bio-Markers for Infection, the Centre of Excellent for Advanced Science, National Research CentreResearch Group Immune- and Bio-Markers for Infection, the Centre of Excellent for Advanced Science, National Research CentreEnvironmental Virology Laboratory, Department of Water Pollution Research, Environmental and Climate Change Research Institute, National Research CentreResearch Group Immune- and Bio-Markers for Infection, the Centre of Excellent for Advanced Science, National Research CentreAbstract An integrated membrane filtration system was developed to make water purity suitable for microbiology/molecular biology research. Water samples were collected from outlets in different buildings of the National Research Center and analyzed for their composition before filtration. An integrated membrane system was developed based on mathematical modeling. Flat sheet membranes were produced, including microfiltration, ultrafiltration, and nanofiltration membranes. The flat sheet membranes were converted to a spiral wound module filter to simulate the local market filters and applied in the integrated membrane system that was designed and installed. The produced water was analyzed and compared to molecular-grade water used in different molecular biology/microbiology applications. Both PCR, agarose gel electrophoresis, bacterial liquid cultures, and viral propagation indicated that treated water using the herein-developed system exhibited comparable performance to the molecular grade water provided with imported reagent kits. So, this research can offer a promising solution for producing high-quality water suitable for sensitive laboratory applications.https://doi.org/10.1007/s13201-024-02294-0Integrated filtration systemWater analysisMembrane preparationMolecular biology/microbiology applications |
| spellingShingle | Heba Abdallah Dina Nadeem Abd-Elshafy Marwa Shalaby Kareem Awad Rola Nadeem Ahmed Shaban Mahmoud Mohamed Bahgat Integrated membranes system for water application in microbiology/molecular biology Applied Water Science Integrated filtration system Water analysis Membrane preparation Molecular biology/microbiology applications |
| title | Integrated membranes system for water application in microbiology/molecular biology |
| title_full | Integrated membranes system for water application in microbiology/molecular biology |
| title_fullStr | Integrated membranes system for water application in microbiology/molecular biology |
| title_full_unstemmed | Integrated membranes system for water application in microbiology/molecular biology |
| title_short | Integrated membranes system for water application in microbiology/molecular biology |
| title_sort | integrated membranes system for water application in microbiology molecular biology |
| topic | Integrated filtration system Water analysis Membrane preparation Molecular biology/microbiology applications |
| url | https://doi.org/10.1007/s13201-024-02294-0 |
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