Optimized Liquid Medium Formulation for <i>Sanghuangporus vaninii</i> and Biological Activity of the Exopolysaccharides
Aims: <i>Sanghuangporus vaninii</i> (<i>S. vaninii</i>) is a rare medicinal mushroom that is rich in polysaccharides, triterpenes, flavonoids, and other bioactive compounds. It has good potential development value. Methods and Results: We performed single factor experiments a...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/13/22/3574 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846153491670630400 |
|---|---|
| author | Haichen Huang Xiaomin Li Qi Lu Hui Xu Huijuan Sun Junli Zhang Xiaoping Wu Junsheng Fu |
| author_facet | Haichen Huang Xiaomin Li Qi Lu Hui Xu Huijuan Sun Junli Zhang Xiaoping Wu Junsheng Fu |
| author_sort | Haichen Huang |
| collection | DOAJ |
| description | Aims: <i>Sanghuangporus vaninii</i> (<i>S. vaninii</i>) is a rare medicinal mushroom that is rich in polysaccharides, triterpenes, flavonoids, and other bioactive compounds. It has good potential development value. Methods and Results: We performed single factor experiments and Box-Behnken response surface methodology to optimize the liquid fermentation medium formulation for <i>S. vaninii</i> with mycelial biomass as the indicator. The in vitro antioxidant and anti-cancer capacity of the exopolysaccharides of <i>S. vaninii</i> were estimated. The optimal liquid fermentation media composition for the MS-4, MS-6, and MS-8 strains of <i>Sanghuangporus vaninii</i> consisted of 25.86 ± 0.068 g/L maltose, 7.3 ± 0.043 g/L yeast extract, and 0.71 ± 0.005 g/L dandelion powder. The average mycelial biomass of <i>S. vaninii</i> under optimal conditions was 12.61 g/L. The mycelial biomass of the <i>Sanghuangporus vaninii</i> strains in the optimized formulation was 109–191% higher than that obtained with the basic potato dextrose broth (PDB). The exopolysaccharides of <i>Sanghuangporus vaninii</i> exhibited an ABTS radical scavenging activity with an EC<sub>50</sub> of 0.021 ± 0.017 mg/mL and a DPPH radical scavenging activity with an EC<sub>50</sub> of 0.076 ± 0.043 mg/mL. In anti-cancer assays, these exopolysaccharides demonstrated an IC<sub>50</sub> value of 1.98 ± 0.36 mg/mL against PC-3 human prostate cancer cells, indicating significant bioactivity, highlighting their potential as functional food ingredients. Conclusions: In this study, the formula of liquid fermentation of <i>S. vaninii</i> strains was optimized, which lays a theoretical foundation for increasing the yield of <i>S. vaninii</i> and its application in industry. Moreover, our data showed the clinical potential of the <i>S. vaninii</i> exopolysaccharides as antioxidants and anti-cancer drugs. |
| format | Article |
| id | doaj-art-2828e801f8c1494a86248f44eaf6b9f4 |
| institution | Kabale University |
| issn | 2304-8158 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj-art-2828e801f8c1494a86248f44eaf6b9f42024-11-26T18:04:24ZengMDPI AGFoods2304-81582024-11-011322357410.3390/foods13223574Optimized Liquid Medium Formulation for <i>Sanghuangporus vaninii</i> and Biological Activity of the ExopolysaccharidesHaichen Huang0Xiaomin Li1Qi Lu2Hui Xu3Huijuan Sun4Junli Zhang5Xiaoping Wu6Junsheng Fu7College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaTibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850030, ChinaTibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850030, ChinaCollege of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaAims: <i>Sanghuangporus vaninii</i> (<i>S. vaninii</i>) is a rare medicinal mushroom that is rich in polysaccharides, triterpenes, flavonoids, and other bioactive compounds. It has good potential development value. Methods and Results: We performed single factor experiments and Box-Behnken response surface methodology to optimize the liquid fermentation medium formulation for <i>S. vaninii</i> with mycelial biomass as the indicator. The in vitro antioxidant and anti-cancer capacity of the exopolysaccharides of <i>S. vaninii</i> were estimated. The optimal liquid fermentation media composition for the MS-4, MS-6, and MS-8 strains of <i>Sanghuangporus vaninii</i> consisted of 25.86 ± 0.068 g/L maltose, 7.3 ± 0.043 g/L yeast extract, and 0.71 ± 0.005 g/L dandelion powder. The average mycelial biomass of <i>S. vaninii</i> under optimal conditions was 12.61 g/L. The mycelial biomass of the <i>Sanghuangporus vaninii</i> strains in the optimized formulation was 109–191% higher than that obtained with the basic potato dextrose broth (PDB). The exopolysaccharides of <i>Sanghuangporus vaninii</i> exhibited an ABTS radical scavenging activity with an EC<sub>50</sub> of 0.021 ± 0.017 mg/mL and a DPPH radical scavenging activity with an EC<sub>50</sub> of 0.076 ± 0.043 mg/mL. In anti-cancer assays, these exopolysaccharides demonstrated an IC<sub>50</sub> value of 1.98 ± 0.36 mg/mL against PC-3 human prostate cancer cells, indicating significant bioactivity, highlighting their potential as functional food ingredients. Conclusions: In this study, the formula of liquid fermentation of <i>S. vaninii</i> strains was optimized, which lays a theoretical foundation for increasing the yield of <i>S. vaninii</i> and its application in industry. Moreover, our data showed the clinical potential of the <i>S. vaninii</i> exopolysaccharides as antioxidants and anti-cancer drugs.https://www.mdpi.com/2304-8158/13/22/3574<i>Sanghuangporus vaninii</i>extracellular polysaccharidefermentation mediumantioxidantanticancer |
| spellingShingle | Haichen Huang Xiaomin Li Qi Lu Hui Xu Huijuan Sun Junli Zhang Xiaoping Wu Junsheng Fu Optimized Liquid Medium Formulation for <i>Sanghuangporus vaninii</i> and Biological Activity of the Exopolysaccharides Foods <i>Sanghuangporus vaninii</i> extracellular polysaccharide fermentation medium antioxidant anticancer |
| title | Optimized Liquid Medium Formulation for <i>Sanghuangporus vaninii</i> and Biological Activity of the Exopolysaccharides |
| title_full | Optimized Liquid Medium Formulation for <i>Sanghuangporus vaninii</i> and Biological Activity of the Exopolysaccharides |
| title_fullStr | Optimized Liquid Medium Formulation for <i>Sanghuangporus vaninii</i> and Biological Activity of the Exopolysaccharides |
| title_full_unstemmed | Optimized Liquid Medium Formulation for <i>Sanghuangporus vaninii</i> and Biological Activity of the Exopolysaccharides |
| title_short | Optimized Liquid Medium Formulation for <i>Sanghuangporus vaninii</i> and Biological Activity of the Exopolysaccharides |
| title_sort | optimized liquid medium formulation for i sanghuangporus vaninii i and biological activity of the exopolysaccharides |
| topic | <i>Sanghuangporus vaninii</i> extracellular polysaccharide fermentation medium antioxidant anticancer |
| url | https://www.mdpi.com/2304-8158/13/22/3574 |
| work_keys_str_mv | AT haichenhuang optimizedliquidmediumformulationforisanghuangporusvaniniiiandbiologicalactivityoftheexopolysaccharides AT xiaominli optimizedliquidmediumformulationforisanghuangporusvaniniiiandbiologicalactivityoftheexopolysaccharides AT qilu optimizedliquidmediumformulationforisanghuangporusvaniniiiandbiologicalactivityoftheexopolysaccharides AT huixu optimizedliquidmediumformulationforisanghuangporusvaniniiiandbiologicalactivityoftheexopolysaccharides AT huijuansun optimizedliquidmediumformulationforisanghuangporusvaniniiiandbiologicalactivityoftheexopolysaccharides AT junlizhang optimizedliquidmediumformulationforisanghuangporusvaniniiiandbiologicalactivityoftheexopolysaccharides AT xiaopingwu optimizedliquidmediumformulationforisanghuangporusvaniniiiandbiologicalactivityoftheexopolysaccharides AT junshengfu optimizedliquidmediumformulationforisanghuangporusvaniniiiandbiologicalactivityoftheexopolysaccharides |