Mitochondrial dysfunction induced in human hepatic HepG2 cells exposed to the fungicide kresoxim-methyl and to a mixture kresoxim-methyl/boscalid
The fungicides strobilurins and succinate dehydrogenase inhibitors (SDHIs) are blockers of the electron transport chain (ETC) in fungi. Here, we show that the exposure for 24 h to kresoxym-methyl, a fungicide from the class of strobilurins, alters the mitochondrial respiration in human HepG2 hepatoc...
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Taylor & Francis Group
2024-12-01
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| Series: | Redox Report |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13510002.2024.2424677 |
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| author | Yasmine Vandensande Mélina Carbone Barbara Mathieu Bernard Gallez |
| author_facet | Yasmine Vandensande Mélina Carbone Barbara Mathieu Bernard Gallez |
| author_sort | Yasmine Vandensande |
| collection | DOAJ |
| description | The fungicides strobilurins and succinate dehydrogenase inhibitors (SDHIs) are blockers of the electron transport chain (ETC) in fungi. Here, we show that the exposure for 24 h to kresoxym-methyl, a fungicide from the class of strobilurins, alters the mitochondrial respiration in human HepG2 hepatocytes. In addition, we demonstrate an increase in production of mitochondrial superoxide radical anion, a reduction in ATP level, a decrease in the ratio reduced/oxidized glutathione and a decrease in cell viability (assessed by the LDH assay, Presto Blue assay, and Crystal Violet assay). As kresoxym-methyl is associated to boscalid (SDHI) in commercial formulations, we analyzed a potential exacerbation of the induced mitochondrial dysfunction for this combination. For the highest dose at which kresoxym-methyl (5 µM) and boscalid (0.5 µM) did not induce changes in mitochondrial function when used separately, in contrast, when both fungicides were used in combination at the same concentration, we observed a significant alteration of the mitochondrial function of hepatocytes: there was a decrease in oxygen consumption rate, in the ATP level. In addition, the level of mitochondrial superoxide radical anion was increased leading to a decrease in the ratio reduced/oxidized glutathione, and an increase in viability. |
| format | Article |
| id | doaj-art-280dbd9a7e6f4c1d88bf1a41cc6d1d0b |
| institution | Kabale University |
| issn | 1351-0002 1743-2928 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Redox Report |
| spelling | doaj-art-280dbd9a7e6f4c1d88bf1a41cc6d1d0b2024-12-07T06:20:31ZengTaylor & Francis GroupRedox Report1351-00021743-29282024-12-0129110.1080/13510002.2024.2424677Mitochondrial dysfunction induced in human hepatic HepG2 cells exposed to the fungicide kresoxim-methyl and to a mixture kresoxim-methyl/boscalidYasmine Vandensande0Mélina Carbone1Barbara Mathieu2Bernard Gallez3Biomedical Magnetic Resonance Research Group, Louvain Drug Research Institute, Brussels, BelgiumBiomedical Magnetic Resonance Research Group, Louvain Drug Research Institute, Brussels, BelgiumBiomedical Magnetic Resonance Research Group, Louvain Drug Research Institute, Brussels, BelgiumBiomedical Magnetic Resonance Research Group, Louvain Drug Research Institute, Brussels, BelgiumThe fungicides strobilurins and succinate dehydrogenase inhibitors (SDHIs) are blockers of the electron transport chain (ETC) in fungi. Here, we show that the exposure for 24 h to kresoxym-methyl, a fungicide from the class of strobilurins, alters the mitochondrial respiration in human HepG2 hepatocytes. In addition, we demonstrate an increase in production of mitochondrial superoxide radical anion, a reduction in ATP level, a decrease in the ratio reduced/oxidized glutathione and a decrease in cell viability (assessed by the LDH assay, Presto Blue assay, and Crystal Violet assay). As kresoxym-methyl is associated to boscalid (SDHI) in commercial formulations, we analyzed a potential exacerbation of the induced mitochondrial dysfunction for this combination. For the highest dose at which kresoxym-methyl (5 µM) and boscalid (0.5 µM) did not induce changes in mitochondrial function when used separately, in contrast, when both fungicides were used in combination at the same concentration, we observed a significant alteration of the mitochondrial function of hepatocytes: there was a decrease in oxygen consumption rate, in the ATP level. In addition, the level of mitochondrial superoxide radical anion was increased leading to a decrease in the ratio reduced/oxidized glutathione, and an increase in viability.https://www.tandfonline.com/doi/10.1080/13510002.2024.2424677Fungicidesmitochondrial functionElectron Paramagnetic Resonance (EPR)cocktail effectstrobilurinSDHI |
| spellingShingle | Yasmine Vandensande Mélina Carbone Barbara Mathieu Bernard Gallez Mitochondrial dysfunction induced in human hepatic HepG2 cells exposed to the fungicide kresoxim-methyl and to a mixture kresoxim-methyl/boscalid Redox Report Fungicides mitochondrial function Electron Paramagnetic Resonance (EPR) cocktail effect strobilurin SDHI |
| title | Mitochondrial dysfunction induced in human hepatic HepG2 cells exposed to the fungicide kresoxim-methyl and to a mixture kresoxim-methyl/boscalid |
| title_full | Mitochondrial dysfunction induced in human hepatic HepG2 cells exposed to the fungicide kresoxim-methyl and to a mixture kresoxim-methyl/boscalid |
| title_fullStr | Mitochondrial dysfunction induced in human hepatic HepG2 cells exposed to the fungicide kresoxim-methyl and to a mixture kresoxim-methyl/boscalid |
| title_full_unstemmed | Mitochondrial dysfunction induced in human hepatic HepG2 cells exposed to the fungicide kresoxim-methyl and to a mixture kresoxim-methyl/boscalid |
| title_short | Mitochondrial dysfunction induced in human hepatic HepG2 cells exposed to the fungicide kresoxim-methyl and to a mixture kresoxim-methyl/boscalid |
| title_sort | mitochondrial dysfunction induced in human hepatic hepg2 cells exposed to the fungicide kresoxim methyl and to a mixture kresoxim methyl boscalid |
| topic | Fungicides mitochondrial function Electron Paramagnetic Resonance (EPR) cocktail effect strobilurin SDHI |
| url | https://www.tandfonline.com/doi/10.1080/13510002.2024.2424677 |
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