Protocol for the derivation and culture of murine trophoblast organoids for CRISPR-Cas9 screening

Protocol for the derivation and culture of murine trophoblast organoids for CRISPR-Cas9 screening

Summary: Murine trophoblast organoids present a more balanced array of trophoblast subtypes, rendering them a suitable platform for CRISPR-Cas9-based screening. Here, we present a protocol for the derivation and culture of murine trophoblast organoids from trophoblast stem cells or placentae. We des...

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Bibliographic Details
Main Authors: Qian Mao, Jingwei Jiang, Qinying Ye, Haopeng Wang, Chao-Po Lin
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:STAR Protocols
Subjects:
High-Throughput Screening
CRISPR
Stem Cells
Cell Differentiation
Organoids
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724005707
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