THE ASSOCIATION OF THE POLYMORPHISM AND GENE EXPRESSION OF HEAT SHOCK PROTEIN HSP70 GENE IN WINTER AND SUMMER IN THE SEMEN OF HOLSTEIN BULLS BORN IN IRAQ

The HSP70 protein considered as a molecular markers to resist various stressconditions, especially high temperatures. Therefore, the high level of gene expressionof hsp70 gene is one of the ways to resist stress conditions, which can vary dependingon the polymorphisms. The study aimed to determine t...

Full description

Saved in:
Bibliographic Details
Main Authors: Hassan Nima Habib, Bassam Yasein Khudaier, Amad Falah Hassan, Wessam M M Saleh
Format: Article
Language:English
Published: College of Veterinary Medicine, University of Basrah, Iraq 2018-11-01
Series:Basrah Journal of Veterinary Research
Subjects:
Online Access:https://bjvr.uobasrah.edu.iq/article_172991_f5aaf1b0be58a025883ee8680b5f5602.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849727194218102784
author Hassan Nima Habib
Bassam Yasein Khudaier
Amad Falah Hassan
Wessam M M Saleh
author_facet Hassan Nima Habib
Bassam Yasein Khudaier
Amad Falah Hassan
Wessam M M Saleh
author_sort Hassan Nima Habib
collection DOAJ
description The HSP70 protein considered as a molecular markers to resist various stressconditions, especially high temperatures. Therefore, the high level of gene expressionof hsp70 gene is one of the ways to resist stress conditions, which can vary dependingon the polymorphisms. The study aimed to determine the extent of the correlationbetween gene expression and polymorphisms to hsp70 gene. The study was conductedin the months: November , December , 2015, January 2016, as the winter season andthe months April , May and June, 2016, as the summer season. Twenty nine Holsteinbulls born in Iraq of known fertility, 2.5 – 3.5 years old, back to ArtificialInsemination Center of Abou-Ghareeb Baghdad, Iraq, the General Company forLivestock Services, Semen collected using artificial vagina method, mRNAextraction, Synthesis of mRNA to cDNA and Real-Time Quantitative RT–qPCR weredone. The results showed significant differences in the level of gene expressionbetween polymorphisms to the hsp70 gene. In conclusion there is a positivecorrelation between the gene expression and polymorphisms of hsp70 gene inHolstein bulls born in Iraq .
format Article
id doaj-art-277f04e5647a4bad80fb8d882a22e90c
institution DOAJ
issn 1813-8497
2410-8456
language English
publishDate 2018-11-01
publisher College of Veterinary Medicine, University of Basrah, Iraq
record_format Article
series Basrah Journal of Veterinary Research
spelling doaj-art-277f04e5647a4bad80fb8d882a22e90c2025-08-20T03:09:55ZengCollege of Veterinary Medicine, University of Basrah, IraqBasrah Journal of Veterinary Research1813-84972410-84562018-11-01173 (Proceeding of 6th International Scientific Conference, College of Veterinary Medicine University of Basrah, Iraq)28028910.23975/bjvetr.2018.172991172991THE ASSOCIATION OF THE POLYMORPHISM AND GENE EXPRESSION OF HEAT SHOCK PROTEIN HSP70 GENE IN WINTER AND SUMMER IN THE SEMEN OF HOLSTEIN BULLS BORN IN IRAQHassan Nima Habib0Bassam Yasein Khudaier1Amad Falah Hassan2Wessam M M Saleh3College of Agriculture, University of Basrah, Basrah, IraqCollege of Veterinary, Medicine University of Basrah, Basra, IraqCollege of Agriculture, University of Basrah, Basrah, IraqCollege of Veterinary, Medicine University of Basrah, Basra, IraqThe HSP70 protein considered as a molecular markers to resist various stressconditions, especially high temperatures. Therefore, the high level of gene expressionof hsp70 gene is one of the ways to resist stress conditions, which can vary dependingon the polymorphisms. The study aimed to determine the extent of the correlationbetween gene expression and polymorphisms to hsp70 gene. The study was conductedin the months: November , December , 2015, January 2016, as the winter season andthe months April , May and June, 2016, as the summer season. Twenty nine Holsteinbulls born in Iraq of known fertility, 2.5 – 3.5 years old, back to ArtificialInsemination Center of Abou-Ghareeb Baghdad, Iraq, the General Company forLivestock Services, Semen collected using artificial vagina method, mRNAextraction, Synthesis of mRNA to cDNA and Real-Time Quantitative RT–qPCR weredone. The results showed significant differences in the level of gene expressionbetween polymorphisms to the hsp70 gene. In conclusion there is a positivecorrelation between the gene expression and polymorphisms of hsp70 gene inHolstein bulls born in Iraq .https://bjvr.uobasrah.edu.iq/article_172991_f5aaf1b0be58a025883ee8680b5f5602.pdfhsp70 proteingene expressionpolymorphisms
spellingShingle Hassan Nima Habib
Bassam Yasein Khudaier
Amad Falah Hassan
Wessam M M Saleh
THE ASSOCIATION OF THE POLYMORPHISM AND GENE EXPRESSION OF HEAT SHOCK PROTEIN HSP70 GENE IN WINTER AND SUMMER IN THE SEMEN OF HOLSTEIN BULLS BORN IN IRAQ
Basrah Journal of Veterinary Research
hsp70 protein
gene expression
polymorphisms
title THE ASSOCIATION OF THE POLYMORPHISM AND GENE EXPRESSION OF HEAT SHOCK PROTEIN HSP70 GENE IN WINTER AND SUMMER IN THE SEMEN OF HOLSTEIN BULLS BORN IN IRAQ
title_full THE ASSOCIATION OF THE POLYMORPHISM AND GENE EXPRESSION OF HEAT SHOCK PROTEIN HSP70 GENE IN WINTER AND SUMMER IN THE SEMEN OF HOLSTEIN BULLS BORN IN IRAQ
title_fullStr THE ASSOCIATION OF THE POLYMORPHISM AND GENE EXPRESSION OF HEAT SHOCK PROTEIN HSP70 GENE IN WINTER AND SUMMER IN THE SEMEN OF HOLSTEIN BULLS BORN IN IRAQ
title_full_unstemmed THE ASSOCIATION OF THE POLYMORPHISM AND GENE EXPRESSION OF HEAT SHOCK PROTEIN HSP70 GENE IN WINTER AND SUMMER IN THE SEMEN OF HOLSTEIN BULLS BORN IN IRAQ
title_short THE ASSOCIATION OF THE POLYMORPHISM AND GENE EXPRESSION OF HEAT SHOCK PROTEIN HSP70 GENE IN WINTER AND SUMMER IN THE SEMEN OF HOLSTEIN BULLS BORN IN IRAQ
title_sort association of the polymorphism and gene expression of heat shock protein hsp70 gene in winter and summer in the semen of holstein bulls born in iraq
topic hsp70 protein
gene expression
polymorphisms
url https://bjvr.uobasrah.edu.iq/article_172991_f5aaf1b0be58a025883ee8680b5f5602.pdf
work_keys_str_mv AT hassannimahabib theassociationofthepolymorphismandgeneexpressionofheatshockproteinhsp70geneinwinterandsummerinthesemenofholsteinbullsborniniraq
AT bassamyaseinkhudaier theassociationofthepolymorphismandgeneexpressionofheatshockproteinhsp70geneinwinterandsummerinthesemenofholsteinbullsborniniraq
AT amadfalahhassan theassociationofthepolymorphismandgeneexpressionofheatshockproteinhsp70geneinwinterandsummerinthesemenofholsteinbullsborniniraq
AT wessammmsaleh theassociationofthepolymorphismandgeneexpressionofheatshockproteinhsp70geneinwinterandsummerinthesemenofholsteinbullsborniniraq
AT hassannimahabib associationofthepolymorphismandgeneexpressionofheatshockproteinhsp70geneinwinterandsummerinthesemenofholsteinbullsborniniraq
AT bassamyaseinkhudaier associationofthepolymorphismandgeneexpressionofheatshockproteinhsp70geneinwinterandsummerinthesemenofholsteinbullsborniniraq
AT amadfalahhassan associationofthepolymorphismandgeneexpressionofheatshockproteinhsp70geneinwinterandsummerinthesemenofholsteinbullsborniniraq
AT wessammmsaleh associationofthepolymorphismandgeneexpressionofheatshockproteinhsp70geneinwinterandsummerinthesemenofholsteinbullsborniniraq