Leaf Surface Micromorphology in Hybrids of Wheat and <i>×Trititrigia × Elymus farctus</i>

The leaf surface micromorphology and the size of the stomatal complex of hybrids in the eighth seed generation from the crossing of ×<i>Trititrigia cziczinii</i> × <i>Elymus farctus</i> (f11814) on the wheat-like wheat-wheatgrass hybrid w107 were investigated by performing sc...

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Main Authors: Alexander V. Babosha, Pavla O. Loshakova, Alina A. Pogost, Margarita M. Gevorkyan, Anastasia D. Alenicheva, Galina I. Komarova, Tatyana S. Wineshenker, Irina N. Klimenkova, Vladimir P. Upelniek
Format: Article
Language:English
Published: MDPI AG 2024-10-01
Series:Agronomy
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Online Access:https://www.mdpi.com/2073-4395/14/11/2490
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author Alexander V. Babosha
Pavla O. Loshakova
Alina A. Pogost
Margarita M. Gevorkyan
Anastasia D. Alenicheva
Galina I. Komarova
Tatyana S. Wineshenker
Irina N. Klimenkova
Vladimir P. Upelniek
author_facet Alexander V. Babosha
Pavla O. Loshakova
Alina A. Pogost
Margarita M. Gevorkyan
Anastasia D. Alenicheva
Galina I. Komarova
Tatyana S. Wineshenker
Irina N. Klimenkova
Vladimir P. Upelniek
author_sort Alexander V. Babosha
collection DOAJ
description The leaf surface micromorphology and the size of the stomatal complex of hybrids in the eighth seed generation from the crossing of ×<i>Trititrigia cziczinii</i> × <i>Elymus farctus</i> (f11814) on the wheat-like wheat-wheatgrass hybrid w107 were investigated by performing scanning electron microscopy of frozen samples (cryoSEM). The micromorphological characteristics of the paternal plants (w107) were dominant in the hybrid leaves. Costal long cells with silicified wavy walls, characteristic of w107 but absent in the mother plants f11814 and <i>E. farctus</i>, were observed in all hybrid samples examined. Conversely, shield-shaped prickles, a characteristic feature of <i>E. farctus</i>, were retained only in some hybrids. In addition, the maternal feature of Ω-shaped junctions of long epidermal cells in the intercostal zone was completely absent in hybrids. Quantitative parameters of the stomatal apparatus showed a weak correlation with micromorphological markers. Stomatal density on the adaxial side was inversely correlated with stomatal size, while variation in these parameters on the abaxial side occurred independently. The prevalence of paternal micromorphological traits in the hybrids seems to be a consequence of the elimination of genetic material from <i>E. farctus</i>, analogous to the loss of chromosomes from wild species observed in other distant crosses.
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spelling doaj-art-276aaa9bc5f9408abfbe9d8c994979f12024-11-26T17:44:10ZengMDPI AGAgronomy2073-43952024-10-011411249010.3390/agronomy14112490Leaf Surface Micromorphology in Hybrids of Wheat and <i>×Trititrigia × Elymus farctus</i>Alexander V. Babosha0Pavla O. Loshakova1Alina A. Pogost2Margarita M. Gevorkyan3Anastasia D. Alenicheva4Galina I. Komarova5Tatyana S. Wineshenker6Irina N. Klimenkova7Vladimir P. Upelniek8N.V. Tsitsin Main Botanical Garden of Russian Academy of Sciences, Botanicheskaya 4, 127276 Moscow, RussiaN.V. Tsitsin Main Botanical Garden of Russian Academy of Sciences, Botanicheskaya 4, 127276 Moscow, RussiaN.V. Tsitsin Main Botanical Garden of Russian Academy of Sciences, Botanicheskaya 4, 127276 Moscow, RussiaN.V. Tsitsin Main Botanical Garden of Russian Academy of Sciences, Botanicheskaya 4, 127276 Moscow, RussiaN.V. Tsitsin Main Botanical Garden of Russian Academy of Sciences, Botanicheskaya 4, 127276 Moscow, RussiaN.V. Tsitsin Main Botanical Garden of Russian Academy of Sciences, Botanicheskaya 4, 127276 Moscow, RussiaLibrary for Natural Scieces of Russian Academy of Sciences, Malỳj Znamenskij per., 11/11, 119019 Moscow, RussiaN.V. Tsitsin Main Botanical Garden of Russian Academy of Sciences, Botanicheskaya 4, 127276 Moscow, RussiaN.V. Tsitsin Main Botanical Garden of Russian Academy of Sciences, Botanicheskaya 4, 127276 Moscow, RussiaThe leaf surface micromorphology and the size of the stomatal complex of hybrids in the eighth seed generation from the crossing of ×<i>Trititrigia cziczinii</i> × <i>Elymus farctus</i> (f11814) on the wheat-like wheat-wheatgrass hybrid w107 were investigated by performing scanning electron microscopy of frozen samples (cryoSEM). The micromorphological characteristics of the paternal plants (w107) were dominant in the hybrid leaves. Costal long cells with silicified wavy walls, characteristic of w107 but absent in the mother plants f11814 and <i>E. farctus</i>, were observed in all hybrid samples examined. Conversely, shield-shaped prickles, a characteristic feature of <i>E. farctus</i>, were retained only in some hybrids. In addition, the maternal feature of Ω-shaped junctions of long epidermal cells in the intercostal zone was completely absent in hybrids. Quantitative parameters of the stomatal apparatus showed a weak correlation with micromorphological markers. Stomatal density on the adaxial side was inversely correlated with stomatal size, while variation in these parameters on the abaxial side occurred independently. The prevalence of paternal micromorphological traits in the hybrids seems to be a consequence of the elimination of genetic material from <i>E. farctus</i>, analogous to the loss of chromosomes from wild species observed in other distant crosses.https://www.mdpi.com/2073-4395/14/11/2490wheat×<i>Trititrigia</i><i>Elymus farctus</i>wheat-wheatgrass hybridsleaf surface micromorphologyscanning electron microscopy
spellingShingle Alexander V. Babosha
Pavla O. Loshakova
Alina A. Pogost
Margarita M. Gevorkyan
Anastasia D. Alenicheva
Galina I. Komarova
Tatyana S. Wineshenker
Irina N. Klimenkova
Vladimir P. Upelniek
Leaf Surface Micromorphology in Hybrids of Wheat and <i>×Trititrigia × Elymus farctus</i>
Agronomy
wheat
×<i>Trititrigia</i>
<i>Elymus farctus</i>
wheat-wheatgrass hybrids
leaf surface micromorphology
scanning electron microscopy
title Leaf Surface Micromorphology in Hybrids of Wheat and <i>×Trititrigia × Elymus farctus</i>
title_full Leaf Surface Micromorphology in Hybrids of Wheat and <i>×Trititrigia × Elymus farctus</i>
title_fullStr Leaf Surface Micromorphology in Hybrids of Wheat and <i>×Trititrigia × Elymus farctus</i>
title_full_unstemmed Leaf Surface Micromorphology in Hybrids of Wheat and <i>×Trititrigia × Elymus farctus</i>
title_short Leaf Surface Micromorphology in Hybrids of Wheat and <i>×Trititrigia × Elymus farctus</i>
title_sort leaf surface micromorphology in hybrids of wheat and i trititrigia elymus farctus i
topic wheat
×<i>Trititrigia</i>
<i>Elymus farctus</i>
wheat-wheatgrass hybrids
leaf surface micromorphology
scanning electron microscopy
url https://www.mdpi.com/2073-4395/14/11/2490
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