3DCryoHolder: a new open access 3D printable system to store and transport silicon nitride membranes under cryogenic conditions for spectromicroscopy at low temperature
Data acquisition under cryogenic conditions allows one to avoid unwanted damage caused by beam irradiation. This is particularly important for the study of biological samples at hard X-ray, micro- or nano-probe beamlines, which focus synchrotron radiation to small beam sizes with extremely high flux...
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| Format: | Article |
| Language: | English |
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International Union of Crystallography
2025-01-01
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| Series: | Journal of Synchrotron Radiation |
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| Online Access: | https://journals.iucr.org/paper?S1600577524010919 |
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| author | Antonio Dominguez-Alfaro Carlos Sanchez-Cano |
| author_facet | Antonio Dominguez-Alfaro Carlos Sanchez-Cano |
| author_sort | Antonio Dominguez-Alfaro |
| collection | DOAJ |
| description | Data acquisition under cryogenic conditions allows one to avoid unwanted damage caused by beam irradiation. This is particularly important for the study of biological samples at hard X-ray, micro- or nano-probe beamlines, which focus synchrotron radiation to small beam sizes with extremely high flux densities. Sample preparation methods for cryopreserved specimens have been adapted from electron microscopy, and include the use of silicon nitride membranes as they are easy to handle and possess low X-ray absorption. Yet, currently there are no commercially available methods for the storage and transport of silicon nitride membranes under cryogenic conditions. Here, we introduce and provide the design files of 3DCryoHolder, a system that can be 3D printed in-house for the correct storage and transport of multiple silicon nitride membranes under cryogenic conditions, and is compatible with all commercial plunge-freezing instruments. |
| format | Article |
| id | doaj-art-274ed78e08d74711bd46d01b06458dc2 |
| institution | Kabale University |
| issn | 1600-5775 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | International Union of Crystallography |
| record_format | Article |
| series | Journal of Synchrotron Radiation |
| spelling | doaj-art-274ed78e08d74711bd46d01b06458dc22025-01-07T14:26:38ZengInternational Union of CrystallographyJournal of Synchrotron Radiation1600-57752025-01-0132122522910.1107/S1600577524010919kam50033DCryoHolder: a new open access 3D printable system to store and transport silicon nitride membranes under cryogenic conditions for spectromicroscopy at low temperatureAntonio Dominguez-Alfaro0Carlos Sanchez-Cano1Polimero eta Material Aurreratuak: Fisika, Kimika eta Teknologia, Kimika Fakultatea, Euskal Herriko Unibertsitatea UPV/EHU, Donostia-San Sebastian 20018, SpainPolimero eta Material Aurreratuak: Fisika, Kimika eta Teknologia, Kimika Fakultatea, Euskal Herriko Unibertsitatea UPV/EHU, Donostia-San Sebastian 20018, SpainData acquisition under cryogenic conditions allows one to avoid unwanted damage caused by beam irradiation. This is particularly important for the study of biological samples at hard X-ray, micro- or nano-probe beamlines, which focus synchrotron radiation to small beam sizes with extremely high flux densities. Sample preparation methods for cryopreserved specimens have been adapted from electron microscopy, and include the use of silicon nitride membranes as they are easy to handle and possess low X-ray absorption. Yet, currently there are no commercially available methods for the storage and transport of silicon nitride membranes under cryogenic conditions. Here, we introduce and provide the design files of 3DCryoHolder, a system that can be 3D printed in-house for the correct storage and transport of multiple silicon nitride membranes under cryogenic conditions, and is compatible with all commercial plunge-freezing instruments.https://journals.iucr.org/paper?S1600577524010919x-ray cryo-spectromicroscopycryopreserved samplesstorage and transport3d printing |
| spellingShingle | Antonio Dominguez-Alfaro Carlos Sanchez-Cano 3DCryoHolder: a new open access 3D printable system to store and transport silicon nitride membranes under cryogenic conditions for spectromicroscopy at low temperature Journal of Synchrotron Radiation x-ray cryo-spectromicroscopy cryopreserved samples storage and transport 3d printing |
| title | 3DCryoHolder: a new open access 3D printable system to store and transport silicon nitride membranes under cryogenic conditions for spectromicroscopy at low temperature |
| title_full | 3DCryoHolder: a new open access 3D printable system to store and transport silicon nitride membranes under cryogenic conditions for spectromicroscopy at low temperature |
| title_fullStr | 3DCryoHolder: a new open access 3D printable system to store and transport silicon nitride membranes under cryogenic conditions for spectromicroscopy at low temperature |
| title_full_unstemmed | 3DCryoHolder: a new open access 3D printable system to store and transport silicon nitride membranes under cryogenic conditions for spectromicroscopy at low temperature |
| title_short | 3DCryoHolder: a new open access 3D printable system to store and transport silicon nitride membranes under cryogenic conditions for spectromicroscopy at low temperature |
| title_sort | 3dcryoholder a new open access 3d printable system to store and transport silicon nitride membranes under cryogenic conditions for spectromicroscopy at low temperature |
| topic | x-ray cryo-spectromicroscopy cryopreserved samples storage and transport 3d printing |
| url | https://journals.iucr.org/paper?S1600577524010919 |
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