Magnetically Induced Anisotropic Microstructures on Polyethylene Glycol Hydrogel Facilitate BMSC Alignment and Osteogenic Differentiation
Many tissues exhibit structural anisotropy, which imparts orientation-specific properties and functions. However, recapitulating the cellular patterns found in anisotropic tissues presents a remarkable challenge, particularly when using soft and wet hydrogels. Herein, we develop self-assembled aniso...
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2024-12-01
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| author | Hua Zhang Yang Luo Rong Xu Xu Cao Guanrong Li Shang Chen |
| author_facet | Hua Zhang Yang Luo Rong Xu Xu Cao Guanrong Li Shang Chen |
| author_sort | Hua Zhang |
| collection | DOAJ |
| description | Many tissues exhibit structural anisotropy, which imparts orientation-specific properties and functions. However, recapitulating the cellular patterns found in anisotropic tissues presents a remarkable challenge, particularly when using soft and wet hydrogels. Herein, we develop self-assembled anisotropic magnetic Fe<sub>3</sub>O<sub>4</sub> micropatterns on polyethylene glycol hydrogels utilizing dipole–dipole interactions. Under the influence of a static magnetic field, Fe<sub>3</sub>O<sub>4</sub> nanoparticles align into highly ordered structures with a height of 400–600 nm and a width of 8–10 μm. Furthermore, our layer-by-layer assembly technique enables the creation of oriented micropatterns with varying densities and heights, which can be further manipulated to form three-dimensional structures by adjusting the angle of the magnetic field. These anisotropic magnetic Fe<sub>3</sub>O<sub>4</sub> micropatterns can be applied to various substrates, including treated glass slides, standard glass slides, silicon wafers, and polydimethylsiloxane. The patterned Fe<sub>3</sub>O<sub>4</sub> scaffolds, modified with gold coating, effectively enhance cellular adhesion, orientation, and osteogenic differentiation of bone marrow-derived stem cells, which is crucial for effective tissue repair. Overall, this study presents an efficient strategy for constructing anisotropic Fe<sub>3</sub>O<sub>4</sub> micropattern hydrogels, providing a bioactive platform that significantly enhances cellular functions. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-273ee73e146b4f139810fe62fe01c36c2024-12-27T14:27:57ZengMDPI AGGels2310-28612024-12-01101281410.3390/gels10120814Magnetically Induced Anisotropic Microstructures on Polyethylene Glycol Hydrogel Facilitate BMSC Alignment and Osteogenic DifferentiationHua Zhang0Yang Luo1Rong Xu2Xu Cao3Guanrong Li4Shang Chen5Research Institute of Smart Medicine and Biological Engineering, Health Science Center, Ningbo University, Ningbo 315211, ChinaResearch Institute of Smart Medicine and Biological Engineering, Health Science Center, Ningbo University, Ningbo 315211, ChinaResearch Institute of Smart Medicine and Biological Engineering, Health Science Center, Ningbo University, Ningbo 315211, ChinaResearch Institute of Smart Medicine and Biological Engineering, Health Science Center, Ningbo University, Ningbo 315211, ChinaResearch Institute of Smart Medicine and Biological Engineering, Health Science Center, Ningbo University, Ningbo 315211, ChinaResearch Institute of Smart Medicine and Biological Engineering, Health Science Center, Ningbo University, Ningbo 315211, ChinaMany tissues exhibit structural anisotropy, which imparts orientation-specific properties and functions. However, recapitulating the cellular patterns found in anisotropic tissues presents a remarkable challenge, particularly when using soft and wet hydrogels. Herein, we develop self-assembled anisotropic magnetic Fe<sub>3</sub>O<sub>4</sub> micropatterns on polyethylene glycol hydrogels utilizing dipole–dipole interactions. Under the influence of a static magnetic field, Fe<sub>3</sub>O<sub>4</sub> nanoparticles align into highly ordered structures with a height of 400–600 nm and a width of 8–10 μm. Furthermore, our layer-by-layer assembly technique enables the creation of oriented micropatterns with varying densities and heights, which can be further manipulated to form three-dimensional structures by adjusting the angle of the magnetic field. These anisotropic magnetic Fe<sub>3</sub>O<sub>4</sub> micropatterns can be applied to various substrates, including treated glass slides, standard glass slides, silicon wafers, and polydimethylsiloxane. The patterned Fe<sub>3</sub>O<sub>4</sub> scaffolds, modified with gold coating, effectively enhance cellular adhesion, orientation, and osteogenic differentiation of bone marrow-derived stem cells, which is crucial for effective tissue repair. Overall, this study presents an efficient strategy for constructing anisotropic Fe<sub>3</sub>O<sub>4</sub> micropattern hydrogels, providing a bioactive platform that significantly enhances cellular functions.https://www.mdpi.com/2310-2861/10/12/814magnetic field inductionFe<sub>3</sub>O<sub>4</sub> micropatternsoriented cytoskeletonosteogenic differentiation |
| spellingShingle | Hua Zhang Yang Luo Rong Xu Xu Cao Guanrong Li Shang Chen Magnetically Induced Anisotropic Microstructures on Polyethylene Glycol Hydrogel Facilitate BMSC Alignment and Osteogenic Differentiation Gels magnetic field induction Fe<sub>3</sub>O<sub>4</sub> micropatterns oriented cytoskeleton osteogenic differentiation |
| title | Magnetically Induced Anisotropic Microstructures on Polyethylene Glycol Hydrogel Facilitate BMSC Alignment and Osteogenic Differentiation |
| title_full | Magnetically Induced Anisotropic Microstructures on Polyethylene Glycol Hydrogel Facilitate BMSC Alignment and Osteogenic Differentiation |
| title_fullStr | Magnetically Induced Anisotropic Microstructures on Polyethylene Glycol Hydrogel Facilitate BMSC Alignment and Osteogenic Differentiation |
| title_full_unstemmed | Magnetically Induced Anisotropic Microstructures on Polyethylene Glycol Hydrogel Facilitate BMSC Alignment and Osteogenic Differentiation |
| title_short | Magnetically Induced Anisotropic Microstructures on Polyethylene Glycol Hydrogel Facilitate BMSC Alignment and Osteogenic Differentiation |
| title_sort | magnetically induced anisotropic microstructures on polyethylene glycol hydrogel facilitate bmsc alignment and osteogenic differentiation |
| topic | magnetic field induction Fe<sub>3</sub>O<sub>4</sub> micropatterns oriented cytoskeleton osteogenic differentiation |
| url | https://www.mdpi.com/2310-2861/10/12/814 |
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