MiR-107-3p/Atp6v0e1 contributes to protective effects of two selenium-containing peptides,TSeMMM and SeMDPGQQ on lead-induced neurotoxicity
Two selenium (Se)-containing peptides from Se-enriched rice, TSeMMM and SeMDPGQQ, possess neuroprotective potency against lead (Pb2+)-induced cytotoxicity. However, the crosstalk between mRNA and microRNAs (miRNA) involved in the neuroprotection mechanism remains to be elucidated. In this study, RNA...
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Tsinghua University Press
2024-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250171 |
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| author | Yong Fang Tianhang Huang Jian Wu Xieqi Luo Fengjiao Fan Peng Li Jian Ding Xinyang Sun |
| author_facet | Yong Fang Tianhang Huang Jian Wu Xieqi Luo Fengjiao Fan Peng Li Jian Ding Xinyang Sun |
| author_sort | Yong Fang |
| collection | DOAJ |
| description | Two selenium (Se)-containing peptides from Se-enriched rice, TSeMMM and SeMDPGQQ, possess neuroprotective potency against lead (Pb2+)-induced cytotoxicity. However, the crosstalk between mRNA and microRNAs (miRNA) involved in the neuroprotection mechanism remains to be elucidated. In this study, RNA-sequencing and miRNA-sequencing were used to independently identify differentially expressed mRNAs and small RNAs profi les in Pb2+-treated primary fetal rat cortical neurons and then the correlated miRNA-mRNA target pairs were obtained. It was found that 34 mRNAs related to oxidative phosphorylation could be reversed by pretreatment of TSeMMM and SeMDPGQQ. The protective effect of TSeMMM and SeMDPGQQ was mediated by upregulation of miR-107-3p, which downregulates the ATPase H+ transporting V0 subunit e1 (Atp6v0e1) mRNA level. A zebrafish model was applied to verify the relevance between the targeted mRNA and miRNA by real-time quantitative PCR. The results indicated that miR-107-3p was a potential therapeutic target to achieve neuroprotection of Se-containing peptides via stimulation of Atp6v0e1. |
| format | Article |
| id | doaj-art-272862c84be648f68f23c4aad3fb32e8 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-272862c84be648f68f23c4aad3fb32e82025-01-10T06:56:48ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-07-011342060206710.26599/FSHW.2022.9250171MiR-107-3p/Atp6v0e1 contributes to protective effects of two selenium-containing peptides,TSeMMM and SeMDPGQQ on lead-induced neurotoxicityYong Fang0Tianhang Huang1Jian Wu2Xieqi Luo3Fengjiao Fan4Peng Li5Jian Ding6Xinyang Sun7College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, ChinaCollege of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, ChinaLaboratory of Neurodegenerative and Neuroinjury Diseases, Wuxi School of Medicine, Jiangnan University, Wuxi 214122, ChinaCollege of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, ChinaCollege of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, ChinaCollege of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, ChinaCollege of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, ChinaCollege of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, ChinaTwo selenium (Se)-containing peptides from Se-enriched rice, TSeMMM and SeMDPGQQ, possess neuroprotective potency against lead (Pb2+)-induced cytotoxicity. However, the crosstalk between mRNA and microRNAs (miRNA) involved in the neuroprotection mechanism remains to be elucidated. In this study, RNA-sequencing and miRNA-sequencing were used to independently identify differentially expressed mRNAs and small RNAs profi les in Pb2+-treated primary fetal rat cortical neurons and then the correlated miRNA-mRNA target pairs were obtained. It was found that 34 mRNAs related to oxidative phosphorylation could be reversed by pretreatment of TSeMMM and SeMDPGQQ. The protective effect of TSeMMM and SeMDPGQQ was mediated by upregulation of miR-107-3p, which downregulates the ATPase H+ transporting V0 subunit e1 (Atp6v0e1) mRNA level. A zebrafish model was applied to verify the relevance between the targeted mRNA and miRNA by real-time quantitative PCR. The results indicated that miR-107-3p was a potential therapeutic target to achieve neuroprotection of Se-containing peptides via stimulation of Atp6v0e1.https://www.sciopen.com/article/10.26599/FSHW.2022.9250171leadse-containing peptidesneuroprotectionmirna |
| spellingShingle | Yong Fang Tianhang Huang Jian Wu Xieqi Luo Fengjiao Fan Peng Li Jian Ding Xinyang Sun MiR-107-3p/Atp6v0e1 contributes to protective effects of two selenium-containing peptides,TSeMMM and SeMDPGQQ on lead-induced neurotoxicity Food Science and Human Wellness lead se-containing peptides neuroprotection mirna |
| title | MiR-107-3p/Atp6v0e1 contributes to protective effects of two selenium-containing peptides,TSeMMM and SeMDPGQQ on lead-induced neurotoxicity |
| title_full | MiR-107-3p/Atp6v0e1 contributes to protective effects of two selenium-containing peptides,TSeMMM and SeMDPGQQ on lead-induced neurotoxicity |
| title_fullStr | MiR-107-3p/Atp6v0e1 contributes to protective effects of two selenium-containing peptides,TSeMMM and SeMDPGQQ on lead-induced neurotoxicity |
| title_full_unstemmed | MiR-107-3p/Atp6v0e1 contributes to protective effects of two selenium-containing peptides,TSeMMM and SeMDPGQQ on lead-induced neurotoxicity |
| title_short | MiR-107-3p/Atp6v0e1 contributes to protective effects of two selenium-containing peptides,TSeMMM and SeMDPGQQ on lead-induced neurotoxicity |
| title_sort | mir 107 3p atp6v0e1 contributes to protective effects of two selenium containing peptides tsemmm and semdpgqq on lead induced neurotoxicity |
| topic | lead se-containing peptides neuroprotection mirna |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250171 |
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