Molecular Epidemiology of SARS-CoV-2 Variants in Vaccinated and Non-Vaccinated Patients of Erbil Province, Kurdistan Region-Iraq
Multiple new SARS-CoV-2 variants of concern (VOC) have emerged globally since the onset of the COVID-19 pandemic. With the virus continuing to evolve, more are expected. This emphasizes the need for rapid diagnostic methods for the detection of circulating lineages. Variants-specific real-time rever...
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2024-06-01
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| author | Dlshad Abdullah Hassan Sazan Qadir Maulud Rzgar Farooq Rashid Jivan Qasim Ahmed Rezhna Khider Ali |
| author_facet | Dlshad Abdullah Hassan Sazan Qadir Maulud Rzgar Farooq Rashid Jivan Qasim Ahmed Rezhna Khider Ali |
| author_sort | Dlshad Abdullah Hassan |
| collection | DOAJ |
| description | Multiple new SARS-CoV-2 variants of concern (VOC) have emerged globally since the onset of the COVID-19 pandemic. With the virus continuing to evolve, more are expected. This emphasizes the need for rapid diagnostic methods for the detection of circulating lineages. Variants-specific real-time reverse transcription (rRT)-PCR method can be used as an alternative to genome sequencing, which is expensive and labored for identifying these variants, especially in settings with limited resources. We assessed the prevalence of various SARS-CoV-2 variants spreading in the Erbil province using a diagnostic screening RT-PCR-based method. A total of 144 SARS-CoV-2 positive samples were prospectively tested for known SARS-CoV2 variants using ViroQ® SC2 Variant rRT-PCR. Furthermore, the technique was validated using 25 SARS-CoV-2 negative nasal samples. Out of 144 SARS-CoV-2 positive samples, 118 (81.9%) were B.1.617.2 (Delta), 5 (3.5%) were Epsilon B.1.427/B.1.429, 1(0.7%) was Eta B.1.525, 2(1.4%) were SARS-CoV-2 Wild type, while 18 (12.5%) were undefined variant, and the delta strain was the most prevalent SARS-CoV-2 strain. Our study showed that variant-specific RT-PCR could be a useful tool for the rapid screening of SARS-CoV-2 variants. |
| format | Article |
| id | doaj-art-2704508b8d2c435bbc447f9797a9c51d |
| institution | Kabale University |
| issn | 2960-1428 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | QAASPA Publisher |
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| series | BioMed Target Journal |
| spelling | doaj-art-2704508b8d2c435bbc447f9797a9c51d2025-01-10T21:54:26ZengQAASPA PublisherBioMed Target Journal2960-14282024-06-0121242910.59786/bmtj.21315Molecular Epidemiology of SARS-CoV-2 Variants in Vaccinated and Non-Vaccinated Patients of Erbil Province, Kurdistan Region-IraqDlshad Abdullah Hassan0https://orcid.org/0000-0003-2548-2336Sazan Qadir Maulud1https://orcid.org/0000-0002-2399-3055Rzgar Farooq Rashid2https://orcid.org/0000-0002-0846-9888Jivan Qasim Ahmed3https://orcid.org/0000-0002-1461-712XRezhna Khider Ali4Public Health Laboratory Management-Erbil, Erbil, 44001, IraqDepartment of Biology, College of Education, Salahaddin University-Erbil, 44001, IraqDepartment of Medical Laboratory Science, College of Science, Knowledge University, Erbil 44001, IraqDepartment of Pathology and Microbiology, University of Duhok, Duhok, 42001, IraqDepartment of Biology, College of Education, Salahaddin University-Erbil, 44001, IraqMultiple new SARS-CoV-2 variants of concern (VOC) have emerged globally since the onset of the COVID-19 pandemic. With the virus continuing to evolve, more are expected. This emphasizes the need for rapid diagnostic methods for the detection of circulating lineages. Variants-specific real-time reverse transcription (rRT)-PCR method can be used as an alternative to genome sequencing, which is expensive and labored for identifying these variants, especially in settings with limited resources. We assessed the prevalence of various SARS-CoV-2 variants spreading in the Erbil province using a diagnostic screening RT-PCR-based method. A total of 144 SARS-CoV-2 positive samples were prospectively tested for known SARS-CoV2 variants using ViroQ® SC2 Variant rRT-PCR. Furthermore, the technique was validated using 25 SARS-CoV-2 negative nasal samples. Out of 144 SARS-CoV-2 positive samples, 118 (81.9%) were B.1.617.2 (Delta), 5 (3.5%) were Epsilon B.1.427/B.1.429, 1(0.7%) was Eta B.1.525, 2(1.4%) were SARS-CoV-2 Wild type, while 18 (12.5%) were undefined variant, and the delta strain was the most prevalent SARS-CoV-2 strain. Our study showed that variant-specific RT-PCR could be a useful tool for the rapid screening of SARS-CoV-2 variants.https://qaaspa.com/index.php/bmtj/article/view/15covid-19sars-cov-2variantsrt-pcrerbiliraq |
| spellingShingle | Dlshad Abdullah Hassan Sazan Qadir Maulud Rzgar Farooq Rashid Jivan Qasim Ahmed Rezhna Khider Ali Molecular Epidemiology of SARS-CoV-2 Variants in Vaccinated and Non-Vaccinated Patients of Erbil Province, Kurdistan Region-Iraq BioMed Target Journal covid-19 sars-cov-2 variants rt-pcr erbil iraq |
| title | Molecular Epidemiology of SARS-CoV-2 Variants in Vaccinated and Non-Vaccinated Patients of Erbil Province, Kurdistan Region-Iraq |
| title_full | Molecular Epidemiology of SARS-CoV-2 Variants in Vaccinated and Non-Vaccinated Patients of Erbil Province, Kurdistan Region-Iraq |
| title_fullStr | Molecular Epidemiology of SARS-CoV-2 Variants in Vaccinated and Non-Vaccinated Patients of Erbil Province, Kurdistan Region-Iraq |
| title_full_unstemmed | Molecular Epidemiology of SARS-CoV-2 Variants in Vaccinated and Non-Vaccinated Patients of Erbil Province, Kurdistan Region-Iraq |
| title_short | Molecular Epidemiology of SARS-CoV-2 Variants in Vaccinated and Non-Vaccinated Patients of Erbil Province, Kurdistan Region-Iraq |
| title_sort | molecular epidemiology of sars cov 2 variants in vaccinated and non vaccinated patients of erbil province kurdistan region iraq |
| topic | covid-19 sars-cov-2 variants rt-pcr erbil iraq |
| url | https://qaaspa.com/index.php/bmtj/article/view/15 |
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