Chromosome-level assemblies of the endemic Korean species Abeliophyllum distichum and Forsythia ovata

Abstract Abeliophyllum distichum and Forsythia ovata are closely related species endemic to Korea and are highly valued as ornamental shrubs in the Oleaceae family. A combination of PacBio and Illumina sequencing with Hi-C scaffolding technologies was employed to develop chromosome-level genome asse...

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Main Authors: Hoyeol Jang, Ara Cho, Hyuk-Jin Kim, Haneul Kim, Seung-Hoon Jeong, Sun Mi Huh, Hee-Ju Yu, Dong-Kab Kim, Joo-Hwan Kim, Jeong-Hwan Mun
Format: Article
Language:English
Published: Nature Portfolio 2024-12-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-024-04252-6
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author Hoyeol Jang
Ara Cho
Hyuk-Jin Kim
Haneul Kim
Seung-Hoon Jeong
Sun Mi Huh
Hee-Ju Yu
Dong-Kab Kim
Joo-Hwan Kim
Jeong-Hwan Mun
author_facet Hoyeol Jang
Ara Cho
Hyuk-Jin Kim
Haneul Kim
Seung-Hoon Jeong
Sun Mi Huh
Hee-Ju Yu
Dong-Kab Kim
Joo-Hwan Kim
Jeong-Hwan Mun
author_sort Hoyeol Jang
collection DOAJ
description Abstract Abeliophyllum distichum and Forsythia ovata are closely related species endemic to Korea and are highly valued as ornamental shrubs in the Oleaceae family. A combination of PacBio and Illumina sequencing with Hi-C scaffolding technologies was employed to develop chromosome-level genome assemblies of these species. The assembled genome sizes are 795.72 Mb for A. distichum and 1,108.53 Mb for F. ovata. The assemblies exhibit scaffold N50 lengths of 53.12 Mb and 68.97 Mb, with minimal gaps measuring 323.40 kb and 149.00 kb, and 97.71% and 98.82% BUSCO scores for Embryophyta single-copy orthologs, respectively, indicating high contiguity and completeness. The genomes contain 485.24 Mb and 691.68 Mb of repetitive sequences, 4,926 and 7,175 full-length long terminal repeat retrotransposons, and 49,414 and 57,587 protein-coding genes, respectively. The 14 pseudochromosomes encompass 93.80% of the A. distichum genome and 89.11% of the F. ovata genome, thereby demonstrating one-to-one chromosome-level collinearity. These high-quality genome assemblies serve as invaluable resources for genetic and breeding studies, facilitating a deeper understanding of the evolutionary history of these distinctive species.
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spelling doaj-art-26d6227b939b484ca09778737b15f6c92024-12-22T12:14:28ZengNature PortfolioScientific Data2052-44632024-12-011111910.1038/s41597-024-04252-6Chromosome-level assemblies of the endemic Korean species Abeliophyllum distichum and Forsythia ovataHoyeol Jang0Ara Cho1Hyuk-Jin Kim2Haneul Kim3Seung-Hoon Jeong4Sun Mi Huh5Hee-Ju Yu6Dong-Kab Kim7Joo-Hwan Kim8Jeong-Hwan Mun9Department of Bioscience and Bioinformatics, Myongji UniversityDepartment of Bioscience and Bioinformatics, Myongji UniversityDivision of Forest Biodiversity, Korea National ArboretumDepartment of Bioscience and Bioinformatics, Myongji UniversityDepartment of Bioscience and Bioinformatics, Myongji UniversityDepartment of Medical and Biological Sciences, The Catholic University of KoreaDepartment of Medical and Biological Sciences, The Catholic University of KoreaDivision of Forest Biodiversity, Korea National ArboretumDepartment of Life Science, Gachon UniversityDepartment of Bioscience and Bioinformatics, Myongji UniversityAbstract Abeliophyllum distichum and Forsythia ovata are closely related species endemic to Korea and are highly valued as ornamental shrubs in the Oleaceae family. A combination of PacBio and Illumina sequencing with Hi-C scaffolding technologies was employed to develop chromosome-level genome assemblies of these species. The assembled genome sizes are 795.72 Mb for A. distichum and 1,108.53 Mb for F. ovata. The assemblies exhibit scaffold N50 lengths of 53.12 Mb and 68.97 Mb, with minimal gaps measuring 323.40 kb and 149.00 kb, and 97.71% and 98.82% BUSCO scores for Embryophyta single-copy orthologs, respectively, indicating high contiguity and completeness. The genomes contain 485.24 Mb and 691.68 Mb of repetitive sequences, 4,926 and 7,175 full-length long terminal repeat retrotransposons, and 49,414 and 57,587 protein-coding genes, respectively. The 14 pseudochromosomes encompass 93.80% of the A. distichum genome and 89.11% of the F. ovata genome, thereby demonstrating one-to-one chromosome-level collinearity. These high-quality genome assemblies serve as invaluable resources for genetic and breeding studies, facilitating a deeper understanding of the evolutionary history of these distinctive species.https://doi.org/10.1038/s41597-024-04252-6
spellingShingle Hoyeol Jang
Ara Cho
Hyuk-Jin Kim
Haneul Kim
Seung-Hoon Jeong
Sun Mi Huh
Hee-Ju Yu
Dong-Kab Kim
Joo-Hwan Kim
Jeong-Hwan Mun
Chromosome-level assemblies of the endemic Korean species Abeliophyllum distichum and Forsythia ovata
Scientific Data
title Chromosome-level assemblies of the endemic Korean species Abeliophyllum distichum and Forsythia ovata
title_full Chromosome-level assemblies of the endemic Korean species Abeliophyllum distichum and Forsythia ovata
title_fullStr Chromosome-level assemblies of the endemic Korean species Abeliophyllum distichum and Forsythia ovata
title_full_unstemmed Chromosome-level assemblies of the endemic Korean species Abeliophyllum distichum and Forsythia ovata
title_short Chromosome-level assemblies of the endemic Korean species Abeliophyllum distichum and Forsythia ovata
title_sort chromosome level assemblies of the endemic korean species abeliophyllum distichum and forsythia ovata
url https://doi.org/10.1038/s41597-024-04252-6
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