Enhanced osteogenic potential of spider silk fibroin-based composite scaffolds incorporating carboxymethyl cellulose for bone tissue engineering
This study aimed to investigate the characteristics of composite scaffolds that combine fibroin derived from spider silk and carboxymethyl cellulose (CMC) in the field of bone tissue engineering. Fibroin, obtained from spider silk, serves as a valuable biomaterial and constitutes the primary compone...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Biomaterials and Biosystems |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666534424000163 |
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| author | Woong Jin Lee Kyoungjoo Cho Dayoon Lee Seungmin Lee Hyojae Jeon Aaron Youngjae Kim Gyung Whan Kim |
| author_facet | Woong Jin Lee Kyoungjoo Cho Dayoon Lee Seungmin Lee Hyojae Jeon Aaron Youngjae Kim Gyung Whan Kim |
| author_sort | Woong Jin Lee |
| collection | DOAJ |
| description | This study aimed to investigate the characteristics of composite scaffolds that combine fibroin derived from spider silk and carboxymethyl cellulose (CMC) in the field of bone tissue engineering. Fibroin, obtained from spider silk, serves as a valuable biomaterial and constitutes the primary component of fibrous protein-based spider silk threads. To enhance the binding efficiency in bone formation after scaffold implantation, CMC was integrated into fibroin, aiming to improve the injectability properties of the scaffold in bone substitutes. For bone marrow mesenchymal stem cell (BMSC) tissue engineering, BMSCs isolated from mice were seeded onto the scaffold, and the rate of cell proliferation was assessed. The composite scaffold, with the addition of CMC to fibroin, exhibited superior characteristics compared to scaffolds containing only silks, including porous morphology, porosity, surface wettability, water absorption, and thermal properties. Alkaline phosphatase activity in BMSCs was significantly higher in the CMC-containing scaffold compared to the silk-only scaffold, and the CMC-containing scaffold demonstrated increased expression of osteocyte marker genes and proteins. In conclusion, the biocompatibility and hydrophilicity of CMC-containing scaffolds play essential roles in the growth and proliferation of osteocytes. Furthermore, the CMC-containing scaffold design proposed in this study is expected to have a substantial impact on promoting ossification of BMSCs. |
| format | Article |
| id | doaj-art-26c7e3aa1ecb4cf49a3ae79f49be71eb |
| institution | Kabale University |
| issn | 2666-5344 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomaterials and Biosystems |
| spelling | doaj-art-26c7e3aa1ecb4cf49a3ae79f49be71eb2024-12-12T05:23:21ZengElsevierBiomaterials and Biosystems2666-53442024-12-0116100103Enhanced osteogenic potential of spider silk fibroin-based composite scaffolds incorporating carboxymethyl cellulose for bone tissue engineeringWoong Jin Lee0Kyoungjoo Cho1Dayoon Lee2Seungmin Lee3Hyojae Jeon4Aaron Youngjae Kim5Gyung Whan Kim6Department of Neurology, College of Medicine, Yonsei University, Seoul, South KoreaDepartment of Life Science, Kyonggi University, Suwon, South KoreaDepartment of Neurology, College of Medicine, Yonsei University, Seoul, South KoreaDepartment of Neurology, College of Medicine, Yonsei University, Seoul, South Korea; College of Medicine, Ewha Womans University, Seoul, South KoreaDepartment of Neurology, College of Medicine, Yonsei University, Seoul, South KoreaDepartment of Neurology, College of Medicine, Yonsei University, Seoul, South Korea; Weill Cornell Medicine-Qatar, Doha, QatarDepartment of Neurology, College of Medicine, Yonsei University, Seoul, South Korea; Corresponding author.This study aimed to investigate the characteristics of composite scaffolds that combine fibroin derived from spider silk and carboxymethyl cellulose (CMC) in the field of bone tissue engineering. Fibroin, obtained from spider silk, serves as a valuable biomaterial and constitutes the primary component of fibrous protein-based spider silk threads. To enhance the binding efficiency in bone formation after scaffold implantation, CMC was integrated into fibroin, aiming to improve the injectability properties of the scaffold in bone substitutes. For bone marrow mesenchymal stem cell (BMSC) tissue engineering, BMSCs isolated from mice were seeded onto the scaffold, and the rate of cell proliferation was assessed. The composite scaffold, with the addition of CMC to fibroin, exhibited superior characteristics compared to scaffolds containing only silks, including porous morphology, porosity, surface wettability, water absorption, and thermal properties. Alkaline phosphatase activity in BMSCs was significantly higher in the CMC-containing scaffold compared to the silk-only scaffold, and the CMC-containing scaffold demonstrated increased expression of osteocyte marker genes and proteins. In conclusion, the biocompatibility and hydrophilicity of CMC-containing scaffolds play essential roles in the growth and proliferation of osteocytes. Furthermore, the CMC-containing scaffold design proposed in this study is expected to have a substantial impact on promoting ossification of BMSCs.http://www.sciencedirect.com/science/article/pii/S2666534424000163SilksScaffoldCarboxymethyl cellulose (CMC)OsteocyteRegenerative medicine |
| spellingShingle | Woong Jin Lee Kyoungjoo Cho Dayoon Lee Seungmin Lee Hyojae Jeon Aaron Youngjae Kim Gyung Whan Kim Enhanced osteogenic potential of spider silk fibroin-based composite scaffolds incorporating carboxymethyl cellulose for bone tissue engineering Biomaterials and Biosystems Silks Scaffold Carboxymethyl cellulose (CMC) Osteocyte Regenerative medicine |
| title | Enhanced osteogenic potential of spider silk fibroin-based composite scaffolds incorporating carboxymethyl cellulose for bone tissue engineering |
| title_full | Enhanced osteogenic potential of spider silk fibroin-based composite scaffolds incorporating carboxymethyl cellulose for bone tissue engineering |
| title_fullStr | Enhanced osteogenic potential of spider silk fibroin-based composite scaffolds incorporating carboxymethyl cellulose for bone tissue engineering |
| title_full_unstemmed | Enhanced osteogenic potential of spider silk fibroin-based composite scaffolds incorporating carboxymethyl cellulose for bone tissue engineering |
| title_short | Enhanced osteogenic potential of spider silk fibroin-based composite scaffolds incorporating carboxymethyl cellulose for bone tissue engineering |
| title_sort | enhanced osteogenic potential of spider silk fibroin based composite scaffolds incorporating carboxymethyl cellulose for bone tissue engineering |
| topic | Silks Scaffold Carboxymethyl cellulose (CMC) Osteocyte Regenerative medicine |
| url | http://www.sciencedirect.com/science/article/pii/S2666534424000163 |
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