β-ecdysone/PLGA composite scaffolds promote skull defect healing in diabetic rat
IntroductionDiabetes mellitus often leads to bone metabolism disorders, hindering bone regeneration and delaying the healing of bone defects. β-Ecdysone, a plant-derived hormone known for its wide range of physiological activities, possesses hypoglycemic effects and promotes osteogenic differentiati...
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| Format: | Article |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1536102/full |
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| author | Yicai Luo Ziwei Wu Yingjuan Zhang Yang Qiao Yinge Wei Xuan Yan Xiangyu Ma Xianxian Huang Xiaoxia Zhong Zhimao Ye Xinping Lu Hongbing Liao |
| author_facet | Yicai Luo Ziwei Wu Yingjuan Zhang Yang Qiao Yinge Wei Xuan Yan Xiangyu Ma Xianxian Huang Xiaoxia Zhong Zhimao Ye Xinping Lu Hongbing Liao |
| author_sort | Yicai Luo |
| collection | DOAJ |
| description | IntroductionDiabetes mellitus often leads to bone metabolism disorders, hindering bone regeneration and delaying the healing of bone defects. β-Ecdysone, a plant-derived hormone known for its wide range of physiological activities, possesses hypoglycemic effects and promotes osteogenic differentiation. This study developed a composite PLGA slow-release scaffold loaded with β-ecdysone to enhance its bioavailability through topical administration and to investigate its potential to heal diabetic bone defects.MethodsThe composite scaffolds were fabricated using solution casting/particle leaching and freeze-drying techniques. Then a series of characterizations were subjected to test the performance of composite scaffolds, and in vitro safety of the composite scaffolds was tested by CCK8 assay and live/dead cell staining. Further, micro-CT and histology to evaluate the effect of β-E/PLGA composite scaffolds on healing of skull defects in diabetic rats at 4 and 8 weeks after implantation. Simultaneously, the safety of the scaffolds in vivo was also evaluated.ResultsThe material characterization results indicated that, in comparison to the single-pore size scaffold, the composite scaffold exhibited superior porosity, swelling ratio, drug loading capacity, and mechanical properties. Additionally, the composite scaffolds showed appropriate degradation performance and sustained drug release profiles. The CCK8 cytotoxicity assay and live/dead cell staining demonstrated that BMSCs survived and proliferated on the composite scaffold under both low-glucose and high-glucose conditions. Micro-CT and histological investigation demonstrated that β-E/PLGA composite scaffolds promoted new bone growth in the skull defect region of diabetic rats.ConclusionOverall, these findings suggest that the β-E/PLGA composite scaffolds promote the healing of bone defects in diabetic rats. The combination of β-ecdysone and tissue-engineered scaffolds presents a promising approach for treating diabetes-related bone defects. |
| format | Article |
| id | doaj-art-26b959a9aeeb4313922a5cf556a39431 |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-26b959a9aeeb4313922a5cf556a394312025-01-13T06:11:06ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-01-011210.3389/fbioe.2024.15361021536102β-ecdysone/PLGA composite scaffolds promote skull defect healing in diabetic ratYicai LuoZiwei WuYingjuan ZhangYang QiaoYinge WeiXuan YanXiangyu MaXianxian HuangXiaoxia ZhongZhimao YeXinping LuHongbing LiaoIntroductionDiabetes mellitus often leads to bone metabolism disorders, hindering bone regeneration and delaying the healing of bone defects. β-Ecdysone, a plant-derived hormone known for its wide range of physiological activities, possesses hypoglycemic effects and promotes osteogenic differentiation. This study developed a composite PLGA slow-release scaffold loaded with β-ecdysone to enhance its bioavailability through topical administration and to investigate its potential to heal diabetic bone defects.MethodsThe composite scaffolds were fabricated using solution casting/particle leaching and freeze-drying techniques. Then a series of characterizations were subjected to test the performance of composite scaffolds, and in vitro safety of the composite scaffolds was tested by CCK8 assay and live/dead cell staining. Further, micro-CT and histology to evaluate the effect of β-E/PLGA composite scaffolds on healing of skull defects in diabetic rats at 4 and 8 weeks after implantation. Simultaneously, the safety of the scaffolds in vivo was also evaluated.ResultsThe material characterization results indicated that, in comparison to the single-pore size scaffold, the composite scaffold exhibited superior porosity, swelling ratio, drug loading capacity, and mechanical properties. Additionally, the composite scaffolds showed appropriate degradation performance and sustained drug release profiles. The CCK8 cytotoxicity assay and live/dead cell staining demonstrated that BMSCs survived and proliferated on the composite scaffold under both low-glucose and high-glucose conditions. Micro-CT and histological investigation demonstrated that β-E/PLGA composite scaffolds promoted new bone growth in the skull defect region of diabetic rats.ConclusionOverall, these findings suggest that the β-E/PLGA composite scaffolds promote the healing of bone defects in diabetic rats. The combination of β-ecdysone and tissue-engineered scaffolds presents a promising approach for treating diabetes-related bone defects.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1536102/fulldiabetesβ-ecdysonePLGAtissue engineering scaffolddiabetic rat skull defect model |
| spellingShingle | Yicai Luo Ziwei Wu Yingjuan Zhang Yang Qiao Yinge Wei Xuan Yan Xiangyu Ma Xianxian Huang Xiaoxia Zhong Zhimao Ye Xinping Lu Hongbing Liao β-ecdysone/PLGA composite scaffolds promote skull defect healing in diabetic rat Frontiers in Bioengineering and Biotechnology diabetes β-ecdysone PLGA tissue engineering scaffold diabetic rat skull defect model |
| title | β-ecdysone/PLGA composite scaffolds promote skull defect healing in diabetic rat |
| title_full | β-ecdysone/PLGA composite scaffolds promote skull defect healing in diabetic rat |
| title_fullStr | β-ecdysone/PLGA composite scaffolds promote skull defect healing in diabetic rat |
| title_full_unstemmed | β-ecdysone/PLGA composite scaffolds promote skull defect healing in diabetic rat |
| title_short | β-ecdysone/PLGA composite scaffolds promote skull defect healing in diabetic rat |
| title_sort | β ecdysone plga composite scaffolds promote skull defect healing in diabetic rat |
| topic | diabetes β-ecdysone PLGA tissue engineering scaffold diabetic rat skull defect model |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1536102/full |
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