Distinct phenotypes in the preeclamptic-like mouse model induced by adenovirus carrying sFlt1 and recombinant sFlt1 protein
Abstract Background Preeclampsia (PE) is a pregnancy-specific, multisystemic disorder that affects 2–8% pregnancies worldwide and is a leading cause of maternal and perinatal mortality. At present, there is no cure for PE apart from delivery the placenta. Therefore, it is important and urgent to pos...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-12-01
|
| Series: | European Journal of Medical Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40001-024-02223-6 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841559792364027904 |
|---|---|
| author | Yingying Wei Haojun Tian Xuancheng Wei Ai Zhang Mengtian Wei Ruixue Wang Lu Zhang Ping Qiao Kai Wang |
| author_facet | Yingying Wei Haojun Tian Xuancheng Wei Ai Zhang Mengtian Wei Ruixue Wang Lu Zhang Ping Qiao Kai Wang |
| author_sort | Yingying Wei |
| collection | DOAJ |
| description | Abstract Background Preeclampsia (PE) is a pregnancy-specific, multisystemic disorder that affects 2–8% pregnancies worldwide and is a leading cause of maternal and perinatal mortality. At present, there is no cure for PE apart from delivery the placenta. Therefore, it is important and urgent to possess a suitable animal model to study the pathology and treatment of PE. When exogenous soluble fms-like tyrosine kinase-1 (sFlt-1) is administered, pregnant animals develop a PE-like phenotype. However, there is no report on the comparison between different methods of constructing PE mouse models using sFlt-1. Methods In this study, the adenovirus carrying sFlt-1(ADV-Flt-1) and recombinant murine sFlt-1 protein (RM Flt-1) are two different methods were used to induce and compare PE-like mouse models. Pregnancy outcomes were examined on E14.5 and E17.5. Results Our data showed that on E14.5, the adenovirus carrying sFlt-1 induced PE-like phenotype, whereas recombinant murine sFlt-1 protein not. On E17.5, both the two methods induced PE-like phenotype including hypertension, proteinuria, fetal growth restriction, placental and glomerular endotheliosis. Importantly, in the adenoviral-mediated sFlt-1 group, the circulating concentration of sFlt-1 were higher than in the recombinant sFlt-1 group, leading to earlier and more severe symptoms of PE. The ADV-Flt-1 group is easy to operate, quickly effective and efficient. The RM Flt-1 group is safer and more stable, with good repeatability, but slower to take effect. Conclusions We proposed that the adenoviral-mediated sFlt-1 model can better simulate early-onset and severe PE. |
| format | Article |
| id | doaj-art-263b050c12444a5ebaaefd2fd5e4c27f |
| institution | Kabale University |
| issn | 2047-783X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj-art-263b050c12444a5ebaaefd2fd5e4c27f2025-01-05T12:12:16ZengBMCEuropean Journal of Medical Research2047-783X2024-12-0129111210.1186/s40001-024-02223-6Distinct phenotypes in the preeclamptic-like mouse model induced by adenovirus carrying sFlt1 and recombinant sFlt1 proteinYingying Wei0Haojun Tian1Xuancheng Wei2Ai Zhang3Mengtian Wei4Ruixue Wang5Lu Zhang6Ping Qiao7Kai Wang8Clinical and Translational Research Center, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji UniversityClinical and Translational Research Center, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji UniversityClinical and Translational Research Center, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji UniversityFetal Medicine Center, Qingdao Women and Children’s Hospital, Qingdao UniversityClinical and Translational Research Center, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji UniversityClinical and Translational Research Center, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji UniversityClinical and Translational Research Center, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji UniversityDepartment of Obstetrics, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji UniversityClinical and Translational Research Center, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji UniversityAbstract Background Preeclampsia (PE) is a pregnancy-specific, multisystemic disorder that affects 2–8% pregnancies worldwide and is a leading cause of maternal and perinatal mortality. At present, there is no cure for PE apart from delivery the placenta. Therefore, it is important and urgent to possess a suitable animal model to study the pathology and treatment of PE. When exogenous soluble fms-like tyrosine kinase-1 (sFlt-1) is administered, pregnant animals develop a PE-like phenotype. However, there is no report on the comparison between different methods of constructing PE mouse models using sFlt-1. Methods In this study, the adenovirus carrying sFlt-1(ADV-Flt-1) and recombinant murine sFlt-1 protein (RM Flt-1) are two different methods were used to induce and compare PE-like mouse models. Pregnancy outcomes were examined on E14.5 and E17.5. Results Our data showed that on E14.5, the adenovirus carrying sFlt-1 induced PE-like phenotype, whereas recombinant murine sFlt-1 protein not. On E17.5, both the two methods induced PE-like phenotype including hypertension, proteinuria, fetal growth restriction, placental and glomerular endotheliosis. Importantly, in the adenoviral-mediated sFlt-1 group, the circulating concentration of sFlt-1 were higher than in the recombinant sFlt-1 group, leading to earlier and more severe symptoms of PE. The ADV-Flt-1 group is easy to operate, quickly effective and efficient. The RM Flt-1 group is safer and more stable, with good repeatability, but slower to take effect. Conclusions We proposed that the adenoviral-mediated sFlt-1 model can better simulate early-onset and severe PE.https://doi.org/10.1186/s40001-024-02223-6PreeclampsiaAdenovirusSoluble fms-like tyrosine kinase-1MiceModel |
| spellingShingle | Yingying Wei Haojun Tian Xuancheng Wei Ai Zhang Mengtian Wei Ruixue Wang Lu Zhang Ping Qiao Kai Wang Distinct phenotypes in the preeclamptic-like mouse model induced by adenovirus carrying sFlt1 and recombinant sFlt1 protein European Journal of Medical Research Preeclampsia Adenovirus Soluble fms-like tyrosine kinase-1 Mice Model |
| title | Distinct phenotypes in the preeclamptic-like mouse model induced by adenovirus carrying sFlt1 and recombinant sFlt1 protein |
| title_full | Distinct phenotypes in the preeclamptic-like mouse model induced by adenovirus carrying sFlt1 and recombinant sFlt1 protein |
| title_fullStr | Distinct phenotypes in the preeclamptic-like mouse model induced by adenovirus carrying sFlt1 and recombinant sFlt1 protein |
| title_full_unstemmed | Distinct phenotypes in the preeclamptic-like mouse model induced by adenovirus carrying sFlt1 and recombinant sFlt1 protein |
| title_short | Distinct phenotypes in the preeclamptic-like mouse model induced by adenovirus carrying sFlt1 and recombinant sFlt1 protein |
| title_sort | distinct phenotypes in the preeclamptic like mouse model induced by adenovirus carrying sflt1 and recombinant sflt1 protein |
| topic | Preeclampsia Adenovirus Soluble fms-like tyrosine kinase-1 Mice Model |
| url | https://doi.org/10.1186/s40001-024-02223-6 |
| work_keys_str_mv | AT yingyingwei distinctphenotypesinthepreeclampticlikemousemodelinducedbyadenoviruscarryingsflt1andrecombinantsflt1protein AT haojuntian distinctphenotypesinthepreeclampticlikemousemodelinducedbyadenoviruscarryingsflt1andrecombinantsflt1protein AT xuanchengwei distinctphenotypesinthepreeclampticlikemousemodelinducedbyadenoviruscarryingsflt1andrecombinantsflt1protein AT aizhang distinctphenotypesinthepreeclampticlikemousemodelinducedbyadenoviruscarryingsflt1andrecombinantsflt1protein AT mengtianwei distinctphenotypesinthepreeclampticlikemousemodelinducedbyadenoviruscarryingsflt1andrecombinantsflt1protein AT ruixuewang distinctphenotypesinthepreeclampticlikemousemodelinducedbyadenoviruscarryingsflt1andrecombinantsflt1protein AT luzhang distinctphenotypesinthepreeclampticlikemousemodelinducedbyadenoviruscarryingsflt1andrecombinantsflt1protein AT pingqiao distinctphenotypesinthepreeclampticlikemousemodelinducedbyadenoviruscarryingsflt1andrecombinantsflt1protein AT kaiwang distinctphenotypesinthepreeclampticlikemousemodelinducedbyadenoviruscarryingsflt1andrecombinantsflt1protein |