In vitro Assessment of Novel Bioactive Compounds’ Anti-Inflammatory and Wound-Healing Properties for Adjunctive Treatment of Oral Mucosal Lesions and Ulcerations
Background: Oral mucosal lesions and ulcerations, prevalent in various dental and systemic conditions, frequently result in considerable discomfort and healing delays. This study evaluates the potential of rosiglitazone and apigenin as adjunctive treatments, focusing on their anti-inflammatory and w...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-12-01
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| Series: | Journal of Pharmacy and Bioallied Sciences |
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| Online Access: | https://journals.lww.com/10.4103/jpbs.jpbs_1210_24 |
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| author | Saud Naif Albakri Vivek Kumar Sidhu M. Pradeep Reddy Shiana Jo Saloni Verma D Anupama Prasad |
| author_facet | Saud Naif Albakri Vivek Kumar Sidhu M. Pradeep Reddy Shiana Jo Saloni Verma D Anupama Prasad |
| author_sort | Saud Naif Albakri |
| collection | DOAJ |
| description | Background:
Oral mucosal lesions and ulcerations, prevalent in various dental and systemic conditions, frequently result in considerable discomfort and healing delays. This study evaluates the potential of rosiglitazone and apigenin as adjunctive treatments, focusing on their anti-inflammatory and wound-healing properties.
Materials and Methods:
A standardized three-dimensional human oral mucosal model was employed to assess the efficacy of rosiglitazone and apigenin. These compounds were applied in different concentrations and compared against a control group receiving no treatment. The primary outcomes measured were the reduction of inflammation, indicated by cytokine levels (IL-1β, TNF-α) via enzyme-linked immunosorbent assay (ELISA), and the rate of wound healing, evaluated through scratch assays and histological analysis over seven days.
Results:
Rosiglitazone exhibited a significant decrease in cytokine levels, with IL-1β and TNF-α reduced by 40% and 35%, respectively, at 200 μg/mL compared to the control. Apigenin showed a more pronounced reduction, decreasing both cytokines by 50% at a concentration of 250 μg/mL. In terms of wound healing, rosiglitazone and apigenin enhanced closure rates by 60% and 75%, respectively, significantly outperforming the control group, which exhibited only a 25% closure. Histological assessments confirmed increased cellular proliferation and reduced inflammatory infiltration in the treatment groups.
Conclusion:
Rosiglitazone and apigenin demonstrate significant anti-inflammatory and wound-healing effects, suggesting their potential utility as effective adjunctive treatments for oral mucosal lesions and ulcerations. These results support further clinical trials to verify their therapeutic efficacy and safety in human patients. |
| format | Article |
| id | doaj-art-262fb328213c4a46afd4db0872cb013a |
| institution | Kabale University |
| issn | 0976-4879 0975-7406 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Pharmacy and Bioallied Sciences |
| spelling | doaj-art-262fb328213c4a46afd4db0872cb013a2025-01-13T10:24:58ZengWolters Kluwer Medknow PublicationsJournal of Pharmacy and Bioallied Sciences0976-48790975-74062024-12-0116Suppl 4S3761S376310.4103/jpbs.jpbs_1210_24In vitro Assessment of Novel Bioactive Compounds’ Anti-Inflammatory and Wound-Healing Properties for Adjunctive Treatment of Oral Mucosal Lesions and UlcerationsSaud Naif AlbakriVivek Kumar SidhuM. Pradeep ReddyShiana JoSaloni VermaD Anupama PrasadBackground: Oral mucosal lesions and ulcerations, prevalent in various dental and systemic conditions, frequently result in considerable discomfort and healing delays. This study evaluates the potential of rosiglitazone and apigenin as adjunctive treatments, focusing on their anti-inflammatory and wound-healing properties. Materials and Methods: A standardized three-dimensional human oral mucosal model was employed to assess the efficacy of rosiglitazone and apigenin. These compounds were applied in different concentrations and compared against a control group receiving no treatment. The primary outcomes measured were the reduction of inflammation, indicated by cytokine levels (IL-1β, TNF-α) via enzyme-linked immunosorbent assay (ELISA), and the rate of wound healing, evaluated through scratch assays and histological analysis over seven days. Results: Rosiglitazone exhibited a significant decrease in cytokine levels, with IL-1β and TNF-α reduced by 40% and 35%, respectively, at 200 μg/mL compared to the control. Apigenin showed a more pronounced reduction, decreasing both cytokines by 50% at a concentration of 250 μg/mL. In terms of wound healing, rosiglitazone and apigenin enhanced closure rates by 60% and 75%, respectively, significantly outperforming the control group, which exhibited only a 25% closure. Histological assessments confirmed increased cellular proliferation and reduced inflammatory infiltration in the treatment groups. Conclusion: Rosiglitazone and apigenin demonstrate significant anti-inflammatory and wound-healing effects, suggesting their potential utility as effective adjunctive treatments for oral mucosal lesions and ulcerations. These results support further clinical trials to verify their therapeutic efficacy and safety in human patients.https://journals.lww.com/10.4103/jpbs.jpbs_1210_24anti-inflammatoryapigenincytokineselisaoral mucosal lesionsrosiglitazonescratch assaywound healing |
| spellingShingle | Saud Naif Albakri Vivek Kumar Sidhu M. Pradeep Reddy Shiana Jo Saloni Verma D Anupama Prasad In vitro Assessment of Novel Bioactive Compounds’ Anti-Inflammatory and Wound-Healing Properties for Adjunctive Treatment of Oral Mucosal Lesions and Ulcerations Journal of Pharmacy and Bioallied Sciences anti-inflammatory apigenin cytokines elisa oral mucosal lesions rosiglitazone scratch assay wound healing |
| title | In vitro Assessment of Novel Bioactive Compounds’ Anti-Inflammatory and Wound-Healing Properties for Adjunctive Treatment of Oral Mucosal Lesions and Ulcerations |
| title_full | In vitro Assessment of Novel Bioactive Compounds’ Anti-Inflammatory and Wound-Healing Properties for Adjunctive Treatment of Oral Mucosal Lesions and Ulcerations |
| title_fullStr | In vitro Assessment of Novel Bioactive Compounds’ Anti-Inflammatory and Wound-Healing Properties for Adjunctive Treatment of Oral Mucosal Lesions and Ulcerations |
| title_full_unstemmed | In vitro Assessment of Novel Bioactive Compounds’ Anti-Inflammatory and Wound-Healing Properties for Adjunctive Treatment of Oral Mucosal Lesions and Ulcerations |
| title_short | In vitro Assessment of Novel Bioactive Compounds’ Anti-Inflammatory and Wound-Healing Properties for Adjunctive Treatment of Oral Mucosal Lesions and Ulcerations |
| title_sort | in vitro assessment of novel bioactive compounds anti inflammatory and wound healing properties for adjunctive treatment of oral mucosal lesions and ulcerations |
| topic | anti-inflammatory apigenin cytokines elisa oral mucosal lesions rosiglitazone scratch assay wound healing |
| url | https://journals.lww.com/10.4103/jpbs.jpbs_1210_24 |
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