Contemporary comprehensive approaches to assessing the effectiveness of experimental model of neurodegenerative disorders with cognitive status changes

Aim of the study was to characterize the locomotor and cognitive aspects of the behavior of experimental rats under intracerebroventricular colchicine administration in the open field test and the 8-arm radial maze, as well as identify a set of behavioral features of experimental animals that emerge...

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Main Authors: M. V. Danukalo, Yu. M. Kolesnyk, O. V. Hancheva
Format: Article
Language:English
Published: Zaporizhzhia State Medical and Pharmaceutical University 2023-12-01
Series:Сучасні медичні технології
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Online Access:https://zmapo-journal.com/index.php/journal/article/view/297
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author M. V. Danukalo
Yu. M. Kolesnyk
O. V. Hancheva
author_facet M. V. Danukalo
Yu. M. Kolesnyk
O. V. Hancheva
author_sort M. V. Danukalo
collection DOAJ
description Aim of the study was to characterize the locomotor and cognitive aspects of the behavior of experimental rats under intracerebroventricular colchicine administration in the open field test and the 8-arm radial maze, as well as identify a set of behavioral features of experimental animals that emerged during the study. Materials and methods. The study was conducted in two stages on 20 male Wistar rats aged 10–11 months. The first stage involved assessment of initial locomotor activity and cognitive functions in all intact rats. At the next stage, the rats were divided into two experimental groups (n = 10): the first group with intracerebroventricular injection of physiological NaCl solution, and the second group with intracerebroventricular injection of colchicine. In 14 days after the surgery, repeated recording of locomotive and cognitive activity indicators was performed. Results. The locomotor activity characteristics did not statistically differ between the animals before the surgical procedures and the rats of the first group. However, in the second group, the activity indicators were significantly higher than in the respective pre-surgery rats. In the intergroup comparison of the rats that entered the second stage of the experiment, it was found that among all the investigated parameters, only the indicator of high activity duration was significantly higher in the second group compared to the first group. At the same time, the animals in the second group showed significant cognitive impairments compared to the first group, as indicated by significantly lower memory index values, the number of correct entries into the maze arms, and a significantly longer time to make the first correct entry into the maze arm. Conclusions. Intracerebroventricular administration of colchicine to experimental rats is accompanied by increased locomotor activity and impairment of cognitive functions. The administration of a physiological solution is not accompanied by a statistically significant increase in locomotor activity, but it demonstrates a clear tendency to increase, which may indicate a certain influence of the procedure itself. The applied pharmacological model of neurodegeneration with subsequent comprehensive assessment of animal behavior in an open field and an 8-arm radial maze is legitimate and can be used to study the early development of neuroinflammation, neuroapoptosis, and synaptogenesis disorders in the experiment.
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spelling doaj-art-25d735edb92743a380884787f1aba1412025-01-02T22:04:08ZengZaporizhzhia State Medical and Pharmaceutical UniversityСучасні медичні технології2072-93672023-12-014515810.34287/MMT.4(59).2023.7297Contemporary comprehensive approaches to assessing the effectiveness of experimental model of neurodegenerative disorders with cognitive status changesM. V. Danukalo0Yu. M. Kolesnyk1O. V. Hancheva2Zaporizhzhia State Medical and Pharmaceutical University, UkraineZaporizhzhia State Medical and Pharmaceutical University, UkraineZaporizhzhia State Medical and Pharmaceutical University, UkraineAim of the study was to characterize the locomotor and cognitive aspects of the behavior of experimental rats under intracerebroventricular colchicine administration in the open field test and the 8-arm radial maze, as well as identify a set of behavioral features of experimental animals that emerged during the study. Materials and methods. The study was conducted in two stages on 20 male Wistar rats aged 10–11 months. The first stage involved assessment of initial locomotor activity and cognitive functions in all intact rats. At the next stage, the rats were divided into two experimental groups (n = 10): the first group with intracerebroventricular injection of physiological NaCl solution, and the second group with intracerebroventricular injection of colchicine. In 14 days after the surgery, repeated recording of locomotive and cognitive activity indicators was performed. Results. The locomotor activity characteristics did not statistically differ between the animals before the surgical procedures and the rats of the first group. However, in the second group, the activity indicators were significantly higher than in the respective pre-surgery rats. In the intergroup comparison of the rats that entered the second stage of the experiment, it was found that among all the investigated parameters, only the indicator of high activity duration was significantly higher in the second group compared to the first group. At the same time, the animals in the second group showed significant cognitive impairments compared to the first group, as indicated by significantly lower memory index values, the number of correct entries into the maze arms, and a significantly longer time to make the first correct entry into the maze arm. Conclusions. Intracerebroventricular administration of colchicine to experimental rats is accompanied by increased locomotor activity and impairment of cognitive functions. The administration of a physiological solution is not accompanied by a statistically significant increase in locomotor activity, but it demonstrates a clear tendency to increase, which may indicate a certain influence of the procedure itself. The applied pharmacological model of neurodegeneration with subsequent comprehensive assessment of animal behavior in an open field and an 8-arm radial maze is legitimate and can be used to study the early development of neuroinflammation, neuroapoptosis, and synaptogenesis disorders in the experiment.https://zmapo-journal.com/index.php/journal/article/view/297neurodegenerative diseasesaxonal transportcognitive functionsopen fieldradial mazerats
spellingShingle M. V. Danukalo
Yu. M. Kolesnyk
O. V. Hancheva
Contemporary comprehensive approaches to assessing the effectiveness of experimental model of neurodegenerative disorders with cognitive status changes
Сучасні медичні технології
neurodegenerative diseases
axonal transport
cognitive functions
open field
radial maze
rats
title Contemporary comprehensive approaches to assessing the effectiveness of experimental model of neurodegenerative disorders with cognitive status changes
title_full Contemporary comprehensive approaches to assessing the effectiveness of experimental model of neurodegenerative disorders with cognitive status changes
title_fullStr Contemporary comprehensive approaches to assessing the effectiveness of experimental model of neurodegenerative disorders with cognitive status changes
title_full_unstemmed Contemporary comprehensive approaches to assessing the effectiveness of experimental model of neurodegenerative disorders with cognitive status changes
title_short Contemporary comprehensive approaches to assessing the effectiveness of experimental model of neurodegenerative disorders with cognitive status changes
title_sort contemporary comprehensive approaches to assessing the effectiveness of experimental model of neurodegenerative disorders with cognitive status changes
topic neurodegenerative diseases
axonal transport
cognitive functions
open field
radial maze
rats
url https://zmapo-journal.com/index.php/journal/article/view/297
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AT yumkolesnyk contemporarycomprehensiveapproachestoassessingtheeffectivenessofexperimentalmodelofneurodegenerativedisorderswithcognitivestatuschanges
AT ovhancheva contemporarycomprehensiveapproachestoassessingtheeffectivenessofexperimentalmodelofneurodegenerativedisorderswithcognitivestatuschanges