Electrophysiological analysis of hyperkalemic cardiomyocytes using a multielectrode array system

The action potential of cardiomyocytes is controlled by electrolytes in serum such as Na+, K+ and Ca2+. Hyperkalemia, which refers to an abnormally high concentration of K+ in the blood, can induce lethal arrythmia. In this study, the extracellular potentials on a sheet of chick embryonic cardiomyoc...

Full description

Saved in:
Bibliographic Details
Main Authors: Kentaro Kito, Masahito Hayashi, Tomoyuki Kaneko
Format: Article
Language:English
Published: The Biophysical Society of Japan 2024-12-01
Series:Biophysics and Physicobiology
Subjects:
Online Access:https://doi.org/10.2142/biophysico.bppb-v21.0026
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1841553360601219072
author Kentaro Kito
Masahito Hayashi
Tomoyuki Kaneko
author_facet Kentaro Kito
Masahito Hayashi
Tomoyuki Kaneko
author_sort Kentaro Kito
collection DOAJ
description The action potential of cardiomyocytes is controlled by electrolytes in serum such as Na+, K+ and Ca2+. Hyperkalemia, which refers to an abnormally high concentration of K+ in the blood, can induce lethal arrythmia. In this study, the extracellular potentials on a sheet of chick embryonic cardiomyocytes were investigated at increasing K+ concentrations using a multielectrode array system. We observed that the interspike interval (ISI) was prolonged by approximately 3.5 times; dV/dt (the slope of a waveform) was decreased by more than five times; the field potential duration (FPD) was shortened by 20%, and the conduction velocity was about half at 12 mM K+ against the control (4 mM K+). In calcium therapy for hyperkalemia, although the prolongation of ISI under hyperkalemic conditions was restored, the slowing of conduction velocity, the decrease in dV/dt, and the shortening of FPD were not recovered by increasing the extracellular Ca2+ concentration. These findings provide a comprehensive understanding of cardiomyocytes in hyperkalemic conditions. Electrophysiological analysis by varying the extracellular concentrations of multiple types of electrolytes will be useful for the further discussion of the results of this study and for the interpretation of the waveforms obtained by measuring the extracellular potential.
format Article
id doaj-art-25c76d7dfeff415e8c25e04a048fc916
institution Kabale University
issn 2189-4779
language English
publishDate 2024-12-01
publisher The Biophysical Society of Japan
record_format Article
series Biophysics and Physicobiology
spelling doaj-art-25c76d7dfeff415e8c25e04a048fc9162025-01-09T10:11:58ZengThe Biophysical Society of JapanBiophysics and Physicobiology2189-47792024-12-012110.2142/biophysico.bppb-v21.0026Electrophysiological analysis of hyperkalemic cardiomyocytes using a multielectrode array systemKentaro Kito0Masahito Hayashi1Tomoyuki Kaneko2Department of Frontier Bioscience, Graduate School of Science & Engineering, Hosei University, Koganei, Tokyo 184-8584, JapanDepartment of Frontier Bioscience, Graduate School of Science & Engineering, Hosei University, Koganei, Tokyo 184-8584, JapanDepartment of Frontier Bioscience, Graduate School of Science & Engineering, Hosei University, Koganei, Tokyo 184-8584, JapanThe action potential of cardiomyocytes is controlled by electrolytes in serum such as Na+, K+ and Ca2+. Hyperkalemia, which refers to an abnormally high concentration of K+ in the blood, can induce lethal arrythmia. In this study, the extracellular potentials on a sheet of chick embryonic cardiomyocytes were investigated at increasing K+ concentrations using a multielectrode array system. We observed that the interspike interval (ISI) was prolonged by approximately 3.5 times; dV/dt (the slope of a waveform) was decreased by more than five times; the field potential duration (FPD) was shortened by 20%, and the conduction velocity was about half at 12 mM K+ against the control (4 mM K+). In calcium therapy for hyperkalemia, although the prolongation of ISI under hyperkalemic conditions was restored, the slowing of conduction velocity, the decrease in dV/dt, and the shortening of FPD were not recovered by increasing the extracellular Ca2+ concentration. These findings provide a comprehensive understanding of cardiomyocytes in hyperkalemic conditions. Electrophysiological analysis by varying the extracellular concentrations of multiple types of electrolytes will be useful for the further discussion of the results of this study and for the interpretation of the waveforms obtained by measuring the extracellular potential.https://doi.org/10.2142/biophysico.bppb-v21.0026hyperkalemiaextracellular potentialcalcium therapy
spellingShingle Kentaro Kito
Masahito Hayashi
Tomoyuki Kaneko
Electrophysiological analysis of hyperkalemic cardiomyocytes using a multielectrode array system
Biophysics and Physicobiology
hyperkalemia
extracellular potential
calcium therapy
title Electrophysiological analysis of hyperkalemic cardiomyocytes using a multielectrode array system
title_full Electrophysiological analysis of hyperkalemic cardiomyocytes using a multielectrode array system
title_fullStr Electrophysiological analysis of hyperkalemic cardiomyocytes using a multielectrode array system
title_full_unstemmed Electrophysiological analysis of hyperkalemic cardiomyocytes using a multielectrode array system
title_short Electrophysiological analysis of hyperkalemic cardiomyocytes using a multielectrode array system
title_sort electrophysiological analysis of hyperkalemic cardiomyocytes using a multielectrode array system
topic hyperkalemia
extracellular potential
calcium therapy
url https://doi.org/10.2142/biophysico.bppb-v21.0026
work_keys_str_mv AT kentarokito electrophysiologicalanalysisofhyperkalemiccardiomyocytesusingamultielectrodearraysystem
AT masahitohayashi electrophysiologicalanalysisofhyperkalemiccardiomyocytesusingamultielectrodearraysystem
AT tomoyukikaneko electrophysiologicalanalysisofhyperkalemiccardiomyocytesusingamultielectrodearraysystem