Chemical Composition and Biological Activities of St John’s Wort (<i>Hypericum perforatum</i> L.) Essential Oil from Bulgaria
Since ancient times, essential oils obtained from various aromatic plants have been utilized as bioactive ingredients in medicines, foods and cosmetics. The present study aimed to investigate the chemical composition and biological activities of St John’s Wort (<i>Hypericum perforatum</i>...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/14/24/11754 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846105974268493824 |
|---|---|
| author | Yulian Tumbarski Ivan Ivanov Mina Todorova Anelia Gerasimova Ivayla Dincheva Lubomir Makedonski Krastena Nikolova |
| author_facet | Yulian Tumbarski Ivan Ivanov Mina Todorova Anelia Gerasimova Ivayla Dincheva Lubomir Makedonski Krastena Nikolova |
| author_sort | Yulian Tumbarski |
| collection | DOAJ |
| description | Since ancient times, essential oils obtained from various aromatic plants have been utilized as bioactive ingredients in medicines, foods and cosmetics. The present study aimed to investigate the chemical composition and biological activities of St John’s Wort (<i>Hypericum perforatum</i> L.) essential oil (SJW EO) from Bulgaria, which is known to possess various biological properties. Gas chromatography and mass spectrometry (GC–MS) analysis, determination of antioxidant activity (by the ABTS method), an antimicrobial activity test and an <i>in vitro</i> anti-inflammatory activity test were performed. The main classes of compounds identified by GC–MS analysis were monoterpenes (43.55%), followed by sesquiterpenes (36.81%) and alkanes (16.92%). The predominant chemical components of SJW EO were α-pinene (27.52%), followed by β-pinene (10.08%), β-caryophyllene (6.77%), germacrene D (6.37%) and caryophyllene oxide (4.48%). The highest antibacterial activity was observed against the Gram-negative bacteria <i>Klebsiella pneumoniae</i> ATCC 13883 (inhibition zone of 12.0 mm) and <i>Pseudomonas aeruginosa</i> ATCC 9027 (inhibition zone of 11.0 mm). SJW EO exhibited significant <i>in vitro</i> anti-inflammatory activity, as the results demonstrated that its anti-inflammatory effect was stronger than those of the conventional anti-inflammatory drugs Prednisolon Cortico and acetylsalicylic acid (Aspirin), which were used as controls (all in concentration of 1 mg/mL). The obtained results demonstrated that Bulgarian SJW EO can be used as an active ingredient in the composition of new products for the pharmaceutical and cosmetic industries. |
| format | Article |
| id | doaj-art-25c19fd9edd14aed9a15c99e6e8e2221 |
| institution | Kabale University |
| issn | 2076-3417 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj-art-25c19fd9edd14aed9a15c99e6e8e22212024-12-27T14:08:16ZengMDPI AGApplied Sciences2076-34172024-12-0114241175410.3390/app142411754Chemical Composition and Biological Activities of St John’s Wort (<i>Hypericum perforatum</i> L.) Essential Oil from BulgariaYulian Tumbarski0Ivan Ivanov1Mina Todorova2Anelia Gerasimova3Ivayla Dincheva4Lubomir Makedonski5Krastena Nikolova6Department of Microbiology and Biotechnology, University of Food Technologies, 4002 Plovdiv, BulgariaDepartment of Organic Chemistry and Inorganic Chemistry, University of Food Technologies, 4002 Plovdiv, BulgariaDepartment of Organic Chemistry, Paisii Hilendarski University of Plovdiv, 4000 Plovdiv, BulgariaDepartment of Chemistry, Faculty of Pharmacy, Medical University of Varna, 9002 Varna, BulgariaDepartment of Agrobiotechnologies, Agrobioinstitute, Agricultural Academy, 1164 Sofia, BulgariaDepartment of Chemistry, Faculty of Pharmacy, Medical University of Varna, 9002 Varna, BulgariaDepartment of Physics and Biophysics, Faculty of Pharmacy, Medical University of Varna, 9002 Varna, BulgariaSince ancient times, essential oils obtained from various aromatic plants have been utilized as bioactive ingredients in medicines, foods and cosmetics. The present study aimed to investigate the chemical composition and biological activities of St John’s Wort (<i>Hypericum perforatum</i> L.) essential oil (SJW EO) from Bulgaria, which is known to possess various biological properties. Gas chromatography and mass spectrometry (GC–MS) analysis, determination of antioxidant activity (by the ABTS method), an antimicrobial activity test and an <i>in vitro</i> anti-inflammatory activity test were performed. The main classes of compounds identified by GC–MS analysis were monoterpenes (43.55%), followed by sesquiterpenes (36.81%) and alkanes (16.92%). The predominant chemical components of SJW EO were α-pinene (27.52%), followed by β-pinene (10.08%), β-caryophyllene (6.77%), germacrene D (6.37%) and caryophyllene oxide (4.48%). The highest antibacterial activity was observed against the Gram-negative bacteria <i>Klebsiella pneumoniae</i> ATCC 13883 (inhibition zone of 12.0 mm) and <i>Pseudomonas aeruginosa</i> ATCC 9027 (inhibition zone of 11.0 mm). SJW EO exhibited significant <i>in vitro</i> anti-inflammatory activity, as the results demonstrated that its anti-inflammatory effect was stronger than those of the conventional anti-inflammatory drugs Prednisolon Cortico and acetylsalicylic acid (Aspirin), which were used as controls (all in concentration of 1 mg/mL). The obtained results demonstrated that Bulgarian SJW EO can be used as an active ingredient in the composition of new products for the pharmaceutical and cosmetic industries.https://www.mdpi.com/2076-3417/14/24/11754essential oilSt John’s Wort<i>Hypericum perforatum</i>biological activities |
| spellingShingle | Yulian Tumbarski Ivan Ivanov Mina Todorova Anelia Gerasimova Ivayla Dincheva Lubomir Makedonski Krastena Nikolova Chemical Composition and Biological Activities of St John’s Wort (<i>Hypericum perforatum</i> L.) Essential Oil from Bulgaria Applied Sciences essential oil St John’s Wort <i>Hypericum perforatum</i> biological activities |
| title | Chemical Composition and Biological Activities of St John’s Wort (<i>Hypericum perforatum</i> L.) Essential Oil from Bulgaria |
| title_full | Chemical Composition and Biological Activities of St John’s Wort (<i>Hypericum perforatum</i> L.) Essential Oil from Bulgaria |
| title_fullStr | Chemical Composition and Biological Activities of St John’s Wort (<i>Hypericum perforatum</i> L.) Essential Oil from Bulgaria |
| title_full_unstemmed | Chemical Composition and Biological Activities of St John’s Wort (<i>Hypericum perforatum</i> L.) Essential Oil from Bulgaria |
| title_short | Chemical Composition and Biological Activities of St John’s Wort (<i>Hypericum perforatum</i> L.) Essential Oil from Bulgaria |
| title_sort | chemical composition and biological activities of st john s wort i hypericum perforatum i l essential oil from bulgaria |
| topic | essential oil St John’s Wort <i>Hypericum perforatum</i> biological activities |
| url | https://www.mdpi.com/2076-3417/14/24/11754 |
| work_keys_str_mv | AT yuliantumbarski chemicalcompositionandbiologicalactivitiesofstjohnswortihypericumperforatumilessentialoilfrombulgaria AT ivanivanov chemicalcompositionandbiologicalactivitiesofstjohnswortihypericumperforatumilessentialoilfrombulgaria AT minatodorova chemicalcompositionandbiologicalactivitiesofstjohnswortihypericumperforatumilessentialoilfrombulgaria AT aneliagerasimova chemicalcompositionandbiologicalactivitiesofstjohnswortihypericumperforatumilessentialoilfrombulgaria AT ivayladincheva chemicalcompositionandbiologicalactivitiesofstjohnswortihypericumperforatumilessentialoilfrombulgaria AT lubomirmakedonski chemicalcompositionandbiologicalactivitiesofstjohnswortihypericumperforatumilessentialoilfrombulgaria AT krastenanikolova chemicalcompositionandbiologicalactivitiesofstjohnswortihypericumperforatumilessentialoilfrombulgaria |