Elevated levels of cyclophilin A secreted in milk during bovine mastitis
ABSTRACT: Bovine mastitis is an inflammatory disease that primarily occurs when bacteria invade and proliferate in the mammary gland, although it can also be caused by physical trauma. Mastitis results in a decrease in milk yield and quality, causing huge economic losses. Cyclophilin A (CyPA) is a c...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Journal of Dairy Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030224012013 |
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| author | Satoru Takanashi Kozue Yoshimura Yuma Endo Megumi Urakawa Hidetoshi Sato Tao Zhuang Tomohito Hayashi Yoshio Kiku Yuya Nagasawa Haruki Kitazawa Michael T. Rose Yoshinobu Uemoto Kouichi Watanabe Tomonori Nochi Hisashi Aso |
| author_facet | Satoru Takanashi Kozue Yoshimura Yuma Endo Megumi Urakawa Hidetoshi Sato Tao Zhuang Tomohito Hayashi Yoshio Kiku Yuya Nagasawa Haruki Kitazawa Michael T. Rose Yoshinobu Uemoto Kouichi Watanabe Tomonori Nochi Hisashi Aso |
| author_sort | Satoru Takanashi |
| collection | DOAJ |
| description | ABSTRACT: Bovine mastitis is an inflammatory disease that primarily occurs when bacteria invade and proliferate in the mammary gland, although it can also be caused by physical trauma. Mastitis results in a decrease in milk yield and quality, causing huge economic losses. Cyclophilin A (CyPA) is a cytosolic protein known as cyclosporine binding protein. Recent studies have shown that CyPA is secreted from cells and has chemotactic activity, recruiting inflammatory cells and inducing multiple cytokines. In this study, we found that CyPA is detected in milk and is abundantly secreted at the onset of mastitis. A significant correlation was found between SCC and the concentrations of CyPA in milk. To elucidate the relationship between mastitis and CyPA, we gave an intramammary infusion of Staphylococcus aureus to cattle and investigated the attendant CyPA secretion. In S. aureus-infused quarters, we observed an increased expression of CyPA on mammary epithelia and secretion into milk. The temporal profiles of CyPA in milk were synchronous with SCC, and there was a significant correlation between the concentration of CyPA in milk and SCC. These results suggest that CyPA is involved in the migration of immune cells during the onset of mastitis and may be used as a marker for the onset of mastitis. |
| format | Article |
| id | doaj-art-24fc8bd45f38498cbd822d84d2cb2fdd |
| institution | Kabale University |
| issn | 0022-0302 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dairy Science |
| spelling | doaj-art-24fc8bd45f38498cbd822d84d2cb2fdd2024-12-26T08:52:12ZengElsevierJournal of Dairy Science0022-03022025-01-011081835844Elevated levels of cyclophilin A secreted in milk during bovine mastitisSatoru Takanashi0Kozue Yoshimura1Yuma Endo2Megumi Urakawa3Hidetoshi Sato4Tao Zhuang5Tomohito Hayashi6Yoshio Kiku7Yuya Nagasawa8Haruki Kitazawa9Michael T. Rose10Yoshinobu Uemoto11Kouichi Watanabe12Tomonori Nochi13Hisashi Aso14International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, 980-8572, Japan; Laboratory of Animal Functional Morphology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, 980-8572, JapanInternational Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, 980-8572, Japan; Laboratory of Animal Functional Morphology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, 980-8572, JapanInternational Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, 980-8572, Japan; Laboratory of Animal Functional Morphology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, 980-8572, JapanInternational Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, 980-8572, Japan; Laboratory of Animal Functional Morphology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, 980-8572, JapanMiyagi Prefectural Livestock Experiment Station, Osaki, Miyagi, 989-6445, JapanInternational Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, 980-8572, Japan; Laboratory of Animal Functional Morphology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, 980-8572, JapanNational Agriculture and Food Research Organization, National Institute of Animal Health, Sapporo, Hokkaido, 062-0045, JapanNational Agriculture and Food Research Organization, National Institute of Animal Health, Sapporo, Hokkaido, 062-0045, JapanNational Agriculture and Food Research Organization, National Institute of Animal Health, Sapporo, Hokkaido, 062-0045, JapanInternational Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, 980-8572, Japan; Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, 980-8572, JapanTasmanian Institute of Agriculture, University of Tasmania, Sandy Bay, Tasmania 5005, AustraliaAnimal Breeding and Genetics, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, 980-8572, JapanInternational Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, 980-8572, Japan; Laboratory of Animal Functional Morphology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, 980-8572, JapanInternational Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, 980-8572, Japan; Laboratory of Animal Functional Morphology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, 980-8572, JapanInternational Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, 980-8572, Japan; Laboratory of Animal Functional Morphology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, 980-8572, Japan; The Cattle Museum, Maesawa, Oshu, Iwate, 029-4205, Japan; Corresponding authorABSTRACT: Bovine mastitis is an inflammatory disease that primarily occurs when bacteria invade and proliferate in the mammary gland, although it can also be caused by physical trauma. Mastitis results in a decrease in milk yield and quality, causing huge economic losses. Cyclophilin A (CyPA) is a cytosolic protein known as cyclosporine binding protein. Recent studies have shown that CyPA is secreted from cells and has chemotactic activity, recruiting inflammatory cells and inducing multiple cytokines. In this study, we found that CyPA is detected in milk and is abundantly secreted at the onset of mastitis. A significant correlation was found between SCC and the concentrations of CyPA in milk. To elucidate the relationship between mastitis and CyPA, we gave an intramammary infusion of Staphylococcus aureus to cattle and investigated the attendant CyPA secretion. In S. aureus-infused quarters, we observed an increased expression of CyPA on mammary epithelia and secretion into milk. The temporal profiles of CyPA in milk were synchronous with SCC, and there was a significant correlation between the concentration of CyPA in milk and SCC. These results suggest that CyPA is involved in the migration of immune cells during the onset of mastitis and may be used as a marker for the onset of mastitis.http://www.sciencedirect.com/science/article/pii/S0022030224012013cyclophilin Ainflammationcattlemastitis |
| spellingShingle | Satoru Takanashi Kozue Yoshimura Yuma Endo Megumi Urakawa Hidetoshi Sato Tao Zhuang Tomohito Hayashi Yoshio Kiku Yuya Nagasawa Haruki Kitazawa Michael T. Rose Yoshinobu Uemoto Kouichi Watanabe Tomonori Nochi Hisashi Aso Elevated levels of cyclophilin A secreted in milk during bovine mastitis Journal of Dairy Science cyclophilin A inflammation cattle mastitis |
| title | Elevated levels of cyclophilin A secreted in milk during bovine mastitis |
| title_full | Elevated levels of cyclophilin A secreted in milk during bovine mastitis |
| title_fullStr | Elevated levels of cyclophilin A secreted in milk during bovine mastitis |
| title_full_unstemmed | Elevated levels of cyclophilin A secreted in milk during bovine mastitis |
| title_short | Elevated levels of cyclophilin A secreted in milk during bovine mastitis |
| title_sort | elevated levels of cyclophilin a secreted in milk during bovine mastitis |
| topic | cyclophilin A inflammation cattle mastitis |
| url | http://www.sciencedirect.com/science/article/pii/S0022030224012013 |
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