Basic Studies on Radioimmunotargeting of CD133-Positive HCT116 Cancer Stem Cells
As cancer stem cells (CSCs) are postulated to play critical roles in cancer development, including metastasis and recurrence, CSC imaging would provide valuable information for cancer treatment and lead to CSC-targeted therapy. To assess the possibility of in vivo CSC targeting, we conducted basic s...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2012-11-01
|
| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2012.00008 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841561841191354368 |
|---|---|
| author | Zhao-Hui Jin Chizuru Sogawa Takako Furukawa Yuriko Saito Winn Aung Yasuhisa Fujibayashi Tsuneo Saga |
| author_facet | Zhao-Hui Jin Chizuru Sogawa Takako Furukawa Yuriko Saito Winn Aung Yasuhisa Fujibayashi Tsuneo Saga |
| author_sort | Zhao-Hui Jin |
| collection | DOAJ |
| description | As cancer stem cells (CSCs) are postulated to play critical roles in cancer development, including metastasis and recurrence, CSC imaging would provide valuable information for cancer treatment and lead to CSC-targeted therapy. To assess the possibility of in vivo CSC targeting, we conducted basic studies on radioimmunotargeting of cancer cells positive for CD133, a CSC marker recognized in various cancers. Antibodies against CD133 were labeled with 125 I, and their in vitro cell binding properties were tested. Using the same isotype IgG as a control, in vivo biodistribution of the labeled antibody retaining immunoreactivity was examined in mice bearing an HCT116 xenograft in which a population of the cancer cells expressed CD133. Intratumoral distribution of the labeled antibody was examined and compared to the CD133 expression pattern. The 125 I-labeled anti-CD133 antibody showed a modest but significantly higher accumulation in the HCT116 xenograft compared to the control IgG. The intratumoral distribution of the labeled antibody mostly overlapped with the CD133 expression, whereas the control IgG was found in the area close to the necrotic tumor center. Our results indicate that noninvasive in vivo targeting of CSCs could be possible with radiolabeled antibodies against cell membrane markers. |
| format | Article |
| id | doaj-art-24bd04a63d9d4d148f6cb2eefff4130c |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2012-11-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-24bd04a63d9d4d148f6cb2eefff4130c2025-01-03T01:22:46ZengSAGE PublishingMolecular Imaging1536-01212012-11-011110.2310/7290.2012.0000810.2310_7290.2012.00008Basic Studies on Radioimmunotargeting of CD133-Positive HCT116 Cancer Stem CellsZhao-Hui JinChizuru SogawaTakako FurukawaYuriko SaitoWinn AungYasuhisa FujibayashiTsuneo SagaAs cancer stem cells (CSCs) are postulated to play critical roles in cancer development, including metastasis and recurrence, CSC imaging would provide valuable information for cancer treatment and lead to CSC-targeted therapy. To assess the possibility of in vivo CSC targeting, we conducted basic studies on radioimmunotargeting of cancer cells positive for CD133, a CSC marker recognized in various cancers. Antibodies against CD133 were labeled with 125 I, and their in vitro cell binding properties were tested. Using the same isotype IgG as a control, in vivo biodistribution of the labeled antibody retaining immunoreactivity was examined in mice bearing an HCT116 xenograft in which a population of the cancer cells expressed CD133. Intratumoral distribution of the labeled antibody was examined and compared to the CD133 expression pattern. The 125 I-labeled anti-CD133 antibody showed a modest but significantly higher accumulation in the HCT116 xenograft compared to the control IgG. The intratumoral distribution of the labeled antibody mostly overlapped with the CD133 expression, whereas the control IgG was found in the area close to the necrotic tumor center. Our results indicate that noninvasive in vivo targeting of CSCs could be possible with radiolabeled antibodies against cell membrane markers.https://doi.org/10.2310/7290.2012.00008 |
| spellingShingle | Zhao-Hui Jin Chizuru Sogawa Takako Furukawa Yuriko Saito Winn Aung Yasuhisa Fujibayashi Tsuneo Saga Basic Studies on Radioimmunotargeting of CD133-Positive HCT116 Cancer Stem Cells Molecular Imaging |
| title | Basic Studies on Radioimmunotargeting of CD133-Positive HCT116 Cancer Stem
Cells |
| title_full | Basic Studies on Radioimmunotargeting of CD133-Positive HCT116 Cancer Stem
Cells |
| title_fullStr | Basic Studies on Radioimmunotargeting of CD133-Positive HCT116 Cancer Stem
Cells |
| title_full_unstemmed | Basic Studies on Radioimmunotargeting of CD133-Positive HCT116 Cancer Stem
Cells |
| title_short | Basic Studies on Radioimmunotargeting of CD133-Positive HCT116 Cancer Stem
Cells |
| title_sort | basic studies on radioimmunotargeting of cd133 positive hct116 cancer stem cells |
| url | https://doi.org/10.2310/7290.2012.00008 |
| work_keys_str_mv | AT zhaohuijin basicstudiesonradioimmunotargetingofcd133positivehct116cancerstemcells AT chizurusogawa basicstudiesonradioimmunotargetingofcd133positivehct116cancerstemcells AT takakofurukawa basicstudiesonradioimmunotargetingofcd133positivehct116cancerstemcells AT yurikosaito basicstudiesonradioimmunotargetingofcd133positivehct116cancerstemcells AT winnaung basicstudiesonradioimmunotargetingofcd133positivehct116cancerstemcells AT yasuhisafujibayashi basicstudiesonradioimmunotargetingofcd133positivehct116cancerstemcells AT tsuneosaga basicstudiesonradioimmunotargetingofcd133positivehct116cancerstemcells |