Assessment of the Concentration of Transforming Growth Factor Beta 1–3 in Degenerated Intervertebral Discs of the Lumbosacral Region of the Spine
The purpose of this study was to evaluate the feasibility of using the expression profile of transforming growth factor beta (TGF-β-1-3) to assess the progression of L/S spine degenerative disease. The study group consisted of 113 lumbosacral (L/S) intervertebral disc (IVD) degenerative disease pati...
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2024-11-01
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| author | Rafał Staszkiewicz Dorian Gładysz Dawid Sobański Filip Bolechała Edward Golec Małgorzata Sobańska Damian Strojny Artur Turek Beniamin Oskar Grabarek |
| author_facet | Rafał Staszkiewicz Dorian Gładysz Dawid Sobański Filip Bolechała Edward Golec Małgorzata Sobańska Damian Strojny Artur Turek Beniamin Oskar Grabarek |
| author_sort | Rafał Staszkiewicz |
| collection | DOAJ |
| description | The purpose of this study was to evaluate the feasibility of using the expression profile of transforming growth factor beta (TGF-β-1-3) to assess the progression of L/S spine degenerative disease. The study group consisted of 113 lumbosacral (L/S) intervertebral disc (IVD) degenerative disease patients from whom IVDs were collected during a microdiscectomy, whereas the control group consisted of 81 participants from whom IVDs were collected during a forensic autopsy or organ harvesting. Hematoxylin and eosin staining was performed to exclude degenerative changes in the IVDs collected from the control group. The molecular analysis consisted of reverse-transcription real-time quantitative polymerase chain reaction (RT-qPCR), an enzyme-linked immunosorbent assay (ELISA), Western blotting, and an immunohistochemical analysis (IHC). In degenerated IVDs, we noted an overexpression of all TGF-β-1-3 mRNA isoforms with the largest changes observed for TGF-β3 isoforms (fold change (FC) = 19.52 ± 2.87) and the smallest for TGF-β2 (FC = 2.26 ± 0.16). Changes in the transcriptional activity of TGF-β-1-3 were statistically significant (<i>p</i> < 0.05). Significantly higher concentrations of TGF-β1 (2797 ± 132 pg/mL vs. 276 ± 19 pg/mL; <i>p</i> < 0.05), TGF-β2 (1918 ± 176 pg/mL vs. 159 ± 17 pg/mL; <i>p</i> < 0.05), and TGF-β3 (2573 ± 102 pg/mL vs. 152 ± 11 pg/mL) were observed in degenerative IVDs compared with the control samples. Determining the concentration profiles of TGF-β1-3 appears to be a promising monitoring tool for the progression of degenerative disease as well as for evaluating its treatment or developing new treatment strategies with molecular targets. |
| format | Article |
| id | doaj-art-24b9effd46d749f5a6e2bca0d14a2a35 |
| institution | Kabale University |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2024-11-01 |
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| spelling | doaj-art-24b9effd46d749f5a6e2bca0d14a2a352024-11-26T17:58:02ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-11-014611128131282910.3390/cimb46110763Assessment of the Concentration of Transforming Growth Factor Beta 1–3 in Degenerated Intervertebral Discs of the Lumbosacral Region of the SpineRafał Staszkiewicz0Dorian Gładysz1Dawid Sobański2Filip Bolechała3Edward Golec4Małgorzata Sobańska5Damian Strojny6Artur Turek7Beniamin Oskar Grabarek8Collegium Medicum, WSB University, 41-300 Dabrowa Gornicza, PolandDepartment of Neurosurgery, 5th Military Clinical Hospital with the SP ZOZ Polyclinic in Krakow, 30-901 Cracow, PolandCollegium Medicum, WSB University, 41-300 Dabrowa Gornicza, PolandDepartment of Forensic Medicine, Jagiellonian University Medical College, 31-531 Cracow, PolandDepartment of Rehabilitation in Orthopaedics, Faculty of Motor Rehabilitation, Bronisław Czech University of Physical Education, 31-571 Krakow, PolandCollegium Medicum, WSB University, 41-300 Dabrowa Gornicza, PolandCollegium Medicum, WSB University, 41-300 Dabrowa Gornicza, PolandChair and Department of Biopharmacy, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, Katowice, 41-200 Sosnowiec, PolandCollegium Medicum, WSB University, 41-300 Dabrowa Gornicza, PolandThe purpose of this study was to evaluate the feasibility of using the expression profile of transforming growth factor beta (TGF-β-1-3) to assess the progression of L/S spine degenerative disease. The study group consisted of 113 lumbosacral (L/S) intervertebral disc (IVD) degenerative disease patients from whom IVDs were collected during a microdiscectomy, whereas the control group consisted of 81 participants from whom IVDs were collected during a forensic autopsy or organ harvesting. Hematoxylin and eosin staining was performed to exclude degenerative changes in the IVDs collected from the control group. The molecular analysis consisted of reverse-transcription real-time quantitative polymerase chain reaction (RT-qPCR), an enzyme-linked immunosorbent assay (ELISA), Western blotting, and an immunohistochemical analysis (IHC). In degenerated IVDs, we noted an overexpression of all TGF-β-1-3 mRNA isoforms with the largest changes observed for TGF-β3 isoforms (fold change (FC) = 19.52 ± 2.87) and the smallest for TGF-β2 (FC = 2.26 ± 0.16). Changes in the transcriptional activity of TGF-β-1-3 were statistically significant (<i>p</i> < 0.05). Significantly higher concentrations of TGF-β1 (2797 ± 132 pg/mL vs. 276 ± 19 pg/mL; <i>p</i> < 0.05), TGF-β2 (1918 ± 176 pg/mL vs. 159 ± 17 pg/mL; <i>p</i> < 0.05), and TGF-β3 (2573 ± 102 pg/mL vs. 152 ± 11 pg/mL) were observed in degenerative IVDs compared with the control samples. Determining the concentration profiles of TGF-β1-3 appears to be a promising monitoring tool for the progression of degenerative disease as well as for evaluating its treatment or developing new treatment strategies with molecular targets.https://www.mdpi.com/1467-3045/46/11/763intervertebral discintervertebral disc degenerationtransforming growth factor beta |
| spellingShingle | Rafał Staszkiewicz Dorian Gładysz Dawid Sobański Filip Bolechała Edward Golec Małgorzata Sobańska Damian Strojny Artur Turek Beniamin Oskar Grabarek Assessment of the Concentration of Transforming Growth Factor Beta 1–3 in Degenerated Intervertebral Discs of the Lumbosacral Region of the Spine Current Issues in Molecular Biology intervertebral disc intervertebral disc degeneration transforming growth factor beta |
| title | Assessment of the Concentration of Transforming Growth Factor Beta 1–3 in Degenerated Intervertebral Discs of the Lumbosacral Region of the Spine |
| title_full | Assessment of the Concentration of Transforming Growth Factor Beta 1–3 in Degenerated Intervertebral Discs of the Lumbosacral Region of the Spine |
| title_fullStr | Assessment of the Concentration of Transforming Growth Factor Beta 1–3 in Degenerated Intervertebral Discs of the Lumbosacral Region of the Spine |
| title_full_unstemmed | Assessment of the Concentration of Transforming Growth Factor Beta 1–3 in Degenerated Intervertebral Discs of the Lumbosacral Region of the Spine |
| title_short | Assessment of the Concentration of Transforming Growth Factor Beta 1–3 in Degenerated Intervertebral Discs of the Lumbosacral Region of the Spine |
| title_sort | assessment of the concentration of transforming growth factor beta 1 3 in degenerated intervertebral discs of the lumbosacral region of the spine |
| topic | intervertebral disc intervertebral disc degeneration transforming growth factor beta |
| url | https://www.mdpi.com/1467-3045/46/11/763 |
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