Expression and application of a novel Holin Hol6A3 in phage PD6A3 of Acinetobacter baumannii
Abstract Background The current overuse of antibiotics presents significant challenges in treating drug-resistant bacterial infections. Bacteriophages have recently emerged as potential alternatives to antibiotics. Nevertheless, their high specificity and narrow host range limit their clinical appli...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-07-01
|
| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-025-04176-0 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849344843112775680 |
|---|---|
| author | Minle Wu Yang Yang Hanqing Wang Beili Wang Baishen Pan Youhua Xie Wei Guo |
| author_facet | Minle Wu Yang Yang Hanqing Wang Beili Wang Baishen Pan Youhua Xie Wei Guo |
| author_sort | Minle Wu |
| collection | DOAJ |
| description | Abstract Background The current overuse of antibiotics presents significant challenges in treating drug-resistant bacterial infections. Bacteriophages have recently emerged as potential alternatives to antibiotics. Nevertheless, their high specificity and narrow host range limit their clinical application. Methods The entire genome of phage PD6A3 was sequenced. Bioinformatic tools were employed to predict genes and determine protein structures. A prokaryotic expression system for Hol6A3 was established. The antibacterial efficacy, thermal and pH stability, and cytotoxicity of Hol6A3 were thoroughly investigated. Additionally, the in vitro antibacterial application of Holin was also studied. Results This study achieved high-purity expression of Hol6A3, with a purity of 1.47 mg/ml. Biochemical properties revealed that Hol6A3 retained stable activity under various conditions. Hol6A3 exhibited broad-spectrum and potent bactericidal effects against various pathogens and synergistic effects in combating bacterial infections with endolysin Ply6A3. Conclusion Hol6A3 possesses considerable antibacterial potential, capable of degrading a broad spectrum of bacteria, demonstrating robust stability and a low propensity for the development of drug resistance. Consequently, Hol6A3 may emerge as a promising alternative to traditional antibiotics. |
| format | Article |
| id | doaj-art-24a583ce91ca4642b2c4e6a6c6173faa |
| institution | Kabale University |
| issn | 1471-2180 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj-art-24a583ce91ca4642b2c4e6a6c6173faa2025-08-20T03:42:34ZengBMCBMC Microbiology1471-21802025-07-0125111110.1186/s12866-025-04176-0Expression and application of a novel Holin Hol6A3 in phage PD6A3 of Acinetobacter baumanniiMinle Wu0Yang Yang1Hanqing Wang2Beili Wang3Baishen Pan4Youhua Xie5Wei Guo6Department of Laboratory Medicine, Zhongshan Hospital, Fudan UniversityKey Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Fudan UniversityKey Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Fudan UniversityDepartment of Laboratory Medicine, Zhongshan Hospital, Fudan UniversityDepartment of Laboratory Medicine, Zhongshan Hospital, Fudan UniversityKey Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Fudan UniversityDepartment of Laboratory Medicine, Zhongshan Hospital, Fudan UniversityAbstract Background The current overuse of antibiotics presents significant challenges in treating drug-resistant bacterial infections. Bacteriophages have recently emerged as potential alternatives to antibiotics. Nevertheless, their high specificity and narrow host range limit their clinical application. Methods The entire genome of phage PD6A3 was sequenced. Bioinformatic tools were employed to predict genes and determine protein structures. A prokaryotic expression system for Hol6A3 was established. The antibacterial efficacy, thermal and pH stability, and cytotoxicity of Hol6A3 were thoroughly investigated. Additionally, the in vitro antibacterial application of Holin was also studied. Results This study achieved high-purity expression of Hol6A3, with a purity of 1.47 mg/ml. Biochemical properties revealed that Hol6A3 retained stable activity under various conditions. Hol6A3 exhibited broad-spectrum and potent bactericidal effects against various pathogens and synergistic effects in combating bacterial infections with endolysin Ply6A3. Conclusion Hol6A3 possesses considerable antibacterial potential, capable of degrading a broad spectrum of bacteria, demonstrating robust stability and a low propensity for the development of drug resistance. Consequently, Hol6A3 may emerge as a promising alternative to traditional antibiotics.https://doi.org/10.1186/s12866-025-04176-0Acinetobacter baumanniiBacteriophagesEndolysinHolinESKAPE |
| spellingShingle | Minle Wu Yang Yang Hanqing Wang Beili Wang Baishen Pan Youhua Xie Wei Guo Expression and application of a novel Holin Hol6A3 in phage PD6A3 of Acinetobacter baumannii BMC Microbiology Acinetobacter baumannii Bacteriophages Endolysin Holin ESKAPE |
| title | Expression and application of a novel Holin Hol6A3 in phage PD6A3 of Acinetobacter baumannii |
| title_full | Expression and application of a novel Holin Hol6A3 in phage PD6A3 of Acinetobacter baumannii |
| title_fullStr | Expression and application of a novel Holin Hol6A3 in phage PD6A3 of Acinetobacter baumannii |
| title_full_unstemmed | Expression and application of a novel Holin Hol6A3 in phage PD6A3 of Acinetobacter baumannii |
| title_short | Expression and application of a novel Holin Hol6A3 in phage PD6A3 of Acinetobacter baumannii |
| title_sort | expression and application of a novel holin hol6a3 in phage pd6a3 of acinetobacter baumannii |
| topic | Acinetobacter baumannii Bacteriophages Endolysin Holin ESKAPE |
| url | https://doi.org/10.1186/s12866-025-04176-0 |
| work_keys_str_mv | AT minlewu expressionandapplicationofanovelholinhol6a3inphagepd6a3ofacinetobacterbaumannii AT yangyang expressionandapplicationofanovelholinhol6a3inphagepd6a3ofacinetobacterbaumannii AT hanqingwang expressionandapplicationofanovelholinhol6a3inphagepd6a3ofacinetobacterbaumannii AT beiliwang expressionandapplicationofanovelholinhol6a3inphagepd6a3ofacinetobacterbaumannii AT baishenpan expressionandapplicationofanovelholinhol6a3inphagepd6a3ofacinetobacterbaumannii AT youhuaxie expressionandapplicationofanovelholinhol6a3inphagepd6a3ofacinetobacterbaumannii AT weiguo expressionandapplicationofanovelholinhol6a3inphagepd6a3ofacinetobacterbaumannii |