Oxytocin ameliorates lipopolysaccharide-induced acute orchitis model: interplay of oxidative stress and inflammatory pathways
IntroductionLipopolysaccharide (LPS), a constituent of the outer membrane of Gram-negative bacteria, is a powerful inducer of systemic inflammation and has been extensively utilized in experimental models to simulate inflammatory responses and septic disorders. Recent research indicates that oxytoci...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Pharmacology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2024.1506777/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841556702086823936 |
|---|---|
| author | Mohamed El-Sherbiny Hany A. Elkattawy Shimaa Hadhoud Ahmed N. Nasr Ateya M. Ibrahim Ateya M. Ibrahim Omar Z. Ameer Noorhan Alsaleebi Joud Asfari Madaniah O. Zakari Moaz A. Mojaddidi Ehab K. Ali Hailah M. Almohaimeed Ahmed Abdeen Sahar K. Ali Mamdouh Eldesoqui Mamdouh Eldesoqui |
| author_facet | Mohamed El-Sherbiny Hany A. Elkattawy Shimaa Hadhoud Ahmed N. Nasr Ateya M. Ibrahim Ateya M. Ibrahim Omar Z. Ameer Noorhan Alsaleebi Joud Asfari Madaniah O. Zakari Moaz A. Mojaddidi Ehab K. Ali Hailah M. Almohaimeed Ahmed Abdeen Sahar K. Ali Mamdouh Eldesoqui Mamdouh Eldesoqui |
| author_sort | Mohamed El-Sherbiny |
| collection | DOAJ |
| description | IntroductionLipopolysaccharide (LPS), a constituent of the outer membrane of Gram-negative bacteria, is a powerful inducer of systemic inflammation and has been extensively utilized in experimental models to simulate inflammatory responses and septic disorders. Recent research indicates that oxytocin (OXY), a neuropeptide typically linked to social bonding and reproductive functions, may influence inflammatory processes. This work examines the impact of OXY on LPS-induced testicular damage, aiming to elucidate its therapeutic potential in addressing inflammatory disorders and broadening the comprehension of its functions beyond conventional neuroendocrine roles.MethodsEighteen male albino rats were divided into three groups; the control group received no treatment; the LPS group received 0.5 mL of saline solution containing 5 mg/kg LPS intraperitoneally (orchitis model); and the LPS + OXY group received LPS and OXY (0.1 mg/kg) intraperitoneally every 12 h for 72 h.Results and discussionAnimals subjected to LPS were found to have severe orchitis, as evidenced by increased oxidative stress and surging inflammatory mediators (TNF-α, IL-1β, and IL-6), with declined IL-10 levels. Besides, LPS increased the malondialdehyde (MDA) and decreased the glutathione (GSH) levels, inducing an oxidative stress cascade. In addition, there are dramatic increases in the TLR4, MyD88, NF-κB, and PK2/PKR1 protein expression levels. All these events could alter the sperm count, morphology, and testicular architecture.ConclusionInterestingly, OXY could mitigate LPS-induced oxidative damage and inflammation in testicular tissue alongside restoring the disrupted sperm count, motility, and morphology. This therapeutic potential of OXY might be accounted for by its anti-inflammatory, antioxidant, and antiapoptotic activities. |
| format | Article |
| id | doaj-art-249f22c5526145c7af545378bb9267fd |
| institution | Kabale University |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj-art-249f22c5526145c7af545378bb9267fd2025-01-07T06:40:35ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-01-011510.3389/fphar.2024.15067771506777Oxytocin ameliorates lipopolysaccharide-induced acute orchitis model: interplay of oxidative stress and inflammatory pathwaysMohamed El-Sherbiny0Hany A. Elkattawy1Shimaa Hadhoud2Ahmed N. Nasr3Ateya M. Ibrahim4Ateya M. Ibrahim5Omar Z. Ameer6Noorhan Alsaleebi7Joud Asfari8Madaniah O. Zakari9Moaz A. Mojaddidi10Ehab K. Ali11Hailah M. Almohaimeed12Ahmed Abdeen13Sahar K. Ali14Mamdouh Eldesoqui15Mamdouh Eldesoqui16Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, Riyadh, Saudi ArabiaDepartment of Basic Medical Sciences, College of Medicine, AlMaarefa University, Riyadh, Saudi ArabiaDepartment of Physiology, Faculty of Medicine, Zagazig University, Zagazig, EgyptDepartment of Anatomy, Faculty of Medicine, Mansoura University, Mansoura, EgyptCollege of Nursing, Prince Sattam bin Abdulaziz University, Al-Kharj, Saudi ArabiaDepartment of Family and Community Health Nursing, Faculty of Nursing, Port Said University, Port Said, EgyptDepartment of Pharmaceutical Sciences, College of Pharmacy, Alfaisal University, Riyadh, Saudi ArabiaDepartment of Basic Medical Sciences, College of Medicine, AlMaarefa University, Riyadh, Saudi ArabiaDepartment of Basic Medical Sciences, College of Medicine, AlMaarefa University, Riyadh, Saudi ArabiaDepartment of Basic Medical Sciences, College of Medicine, Taibah University, Medinah, Saudi ArabiaDepartment of Basic Medical Sciences, College of Medicine, Taibah University, Medinah, Saudi ArabiaDepartment of Anatomy and Embryology, Faculty of Medicine, AL-Azhar University, New Damietta, EgyptDepartment of Basic Sciences, College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia0Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Toukh, Egypt1Department of Medical Pharmacology, Faculty of Medicine, Zagazig University, Zagazig, EgyptDepartment of Basic Medical Sciences, College of Medicine, AlMaarefa University, Riyadh, Saudi ArabiaDepartment of Anatomy, Faculty of Medicine, Mansoura University, Mansoura, EgyptIntroductionLipopolysaccharide (LPS), a constituent of the outer membrane of Gram-negative bacteria, is a powerful inducer of systemic inflammation and has been extensively utilized in experimental models to simulate inflammatory responses and septic disorders. Recent research indicates that oxytocin (OXY), a neuropeptide typically linked to social bonding and reproductive functions, may influence inflammatory processes. This work examines the impact of OXY on LPS-induced testicular damage, aiming to elucidate its therapeutic potential in addressing inflammatory disorders and broadening the comprehension of its functions beyond conventional neuroendocrine roles.MethodsEighteen male albino rats were divided into three groups; the control group received no treatment; the LPS group received 0.5 mL of saline solution containing 5 mg/kg LPS intraperitoneally (orchitis model); and the LPS + OXY group received LPS and OXY (0.1 mg/kg) intraperitoneally every 12 h for 72 h.Results and discussionAnimals subjected to LPS were found to have severe orchitis, as evidenced by increased oxidative stress and surging inflammatory mediators (TNF-α, IL-1β, and IL-6), with declined IL-10 levels. Besides, LPS increased the malondialdehyde (MDA) and decreased the glutathione (GSH) levels, inducing an oxidative stress cascade. In addition, there are dramatic increases in the TLR4, MyD88, NF-κB, and PK2/PKR1 protein expression levels. All these events could alter the sperm count, morphology, and testicular architecture.ConclusionInterestingly, OXY could mitigate LPS-induced oxidative damage and inflammation in testicular tissue alongside restoring the disrupted sperm count, motility, and morphology. This therapeutic potential of OXY might be accounted for by its anti-inflammatory, antioxidant, and antiapoptotic activities.https://www.frontiersin.org/articles/10.3389/fphar.2024.1506777/fulltesticular injuryhormonal therapyoxidative stressantioxidantinflammationsperm |
| spellingShingle | Mohamed El-Sherbiny Hany A. Elkattawy Shimaa Hadhoud Ahmed N. Nasr Ateya M. Ibrahim Ateya M. Ibrahim Omar Z. Ameer Noorhan Alsaleebi Joud Asfari Madaniah O. Zakari Moaz A. Mojaddidi Ehab K. Ali Hailah M. Almohaimeed Ahmed Abdeen Sahar K. Ali Mamdouh Eldesoqui Mamdouh Eldesoqui Oxytocin ameliorates lipopolysaccharide-induced acute orchitis model: interplay of oxidative stress and inflammatory pathways Frontiers in Pharmacology testicular injury hormonal therapy oxidative stress antioxidant inflammation sperm |
| title | Oxytocin ameliorates lipopolysaccharide-induced acute orchitis model: interplay of oxidative stress and inflammatory pathways |
| title_full | Oxytocin ameliorates lipopolysaccharide-induced acute orchitis model: interplay of oxidative stress and inflammatory pathways |
| title_fullStr | Oxytocin ameliorates lipopolysaccharide-induced acute orchitis model: interplay of oxidative stress and inflammatory pathways |
| title_full_unstemmed | Oxytocin ameliorates lipopolysaccharide-induced acute orchitis model: interplay of oxidative stress and inflammatory pathways |
| title_short | Oxytocin ameliorates lipopolysaccharide-induced acute orchitis model: interplay of oxidative stress and inflammatory pathways |
| title_sort | oxytocin ameliorates lipopolysaccharide induced acute orchitis model interplay of oxidative stress and inflammatory pathways |
| topic | testicular injury hormonal therapy oxidative stress antioxidant inflammation sperm |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2024.1506777/full |
| work_keys_str_mv | AT mohamedelsherbiny oxytocinameliorateslipopolysaccharideinducedacuteorchitismodelinterplayofoxidativestressandinflammatorypathways AT hanyaelkattawy oxytocinameliorateslipopolysaccharideinducedacuteorchitismodelinterplayofoxidativestressandinflammatorypathways AT shimaahadhoud oxytocinameliorateslipopolysaccharideinducedacuteorchitismodelinterplayofoxidativestressandinflammatorypathways AT ahmednnasr oxytocinameliorateslipopolysaccharideinducedacuteorchitismodelinterplayofoxidativestressandinflammatorypathways AT ateyamibrahim oxytocinameliorateslipopolysaccharideinducedacuteorchitismodelinterplayofoxidativestressandinflammatorypathways AT ateyamibrahim oxytocinameliorateslipopolysaccharideinducedacuteorchitismodelinterplayofoxidativestressandinflammatorypathways AT omarzameer oxytocinameliorateslipopolysaccharideinducedacuteorchitismodelinterplayofoxidativestressandinflammatorypathways AT noorhanalsaleebi oxytocinameliorateslipopolysaccharideinducedacuteorchitismodelinterplayofoxidativestressandinflammatorypathways AT joudasfari oxytocinameliorateslipopolysaccharideinducedacuteorchitismodelinterplayofoxidativestressandinflammatorypathways AT madaniahozakari oxytocinameliorateslipopolysaccharideinducedacuteorchitismodelinterplayofoxidativestressandinflammatorypathways AT moazamojaddidi oxytocinameliorateslipopolysaccharideinducedacuteorchitismodelinterplayofoxidativestressandinflammatorypathways AT ehabkali oxytocinameliorateslipopolysaccharideinducedacuteorchitismodelinterplayofoxidativestressandinflammatorypathways AT hailahmalmohaimeed oxytocinameliorateslipopolysaccharideinducedacuteorchitismodelinterplayofoxidativestressandinflammatorypathways AT ahmedabdeen oxytocinameliorateslipopolysaccharideinducedacuteorchitismodelinterplayofoxidativestressandinflammatorypathways AT saharkali oxytocinameliorateslipopolysaccharideinducedacuteorchitismodelinterplayofoxidativestressandinflammatorypathways AT mamdouheldesoqui oxytocinameliorateslipopolysaccharideinducedacuteorchitismodelinterplayofoxidativestressandinflammatorypathways AT mamdouheldesoqui oxytocinameliorateslipopolysaccharideinducedacuteorchitismodelinterplayofoxidativestressandinflammatorypathways |