Serotype diversity of Actinobacillus pleuropneumoniae detected by real-time PCR in clinical and subclinical samples from Spanish pig farms during 2017–2022
Abstract Actinobacillus pleuropneumoniae is the causative agent of porcine pleuropneumonia, a challenging respiratory disease for the global swine industry. Variations in the serotypes associated with clinical disease have been observed in different regions worldwide. This study aimed to provide an...
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2024-12-01
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| Online Access: | https://doi.org/10.1186/s13567-024-01419-2 |
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| author | José Luis Arnal Bernal Marcelo Gottschalk Sonia Lacotoure Celia Sanz Tejero Gema Chacón Pérez Desiree Martín-Jurado Ana Belén Fernández Ros |
| author_facet | José Luis Arnal Bernal Marcelo Gottschalk Sonia Lacotoure Celia Sanz Tejero Gema Chacón Pérez Desiree Martín-Jurado Ana Belén Fernández Ros |
| author_sort | José Luis Arnal Bernal |
| collection | DOAJ |
| description | Abstract Actinobacillus pleuropneumoniae is the causative agent of porcine pleuropneumonia, a challenging respiratory disease for the global swine industry. Variations in the serotypes associated with clinical disease have been observed in different regions worldwide. This study aimed to provide an updated epidemiological assessment of A. pleuropneumoniae serotypes in Spain, incorporating bacterial characterization through serotyping and toxinotyping. Serotypes 9/11, 2, 4, 5, 17, and 13 were frequently identified in diseased animals. Furthermore, qPCR of lung samples from an outbreak, even when samples were pooled, emerged as a robust diagnostic tool, enabling the rapid detection of A. pleuropneumoniae and their serotypes without the need for microbiological isolation. This technology also facilitates serotype monitoring of apparently healthy herds through the testing of oral fluids. The study revealed the frequent simultaneous presence of diverse serotypes within a farm. Serotypes 1, 7, 10, 12, 18, and 19 were frequently found in subclinically infected animals but were rarely detected in acute pleuropneumonia outbreaks in the current study. These results provide valuable information for interpreting the potential virulence of the different serotypes in Spain. However, other predisposing factors and the immune status of the herds such as type of vaccines used when appropriate, should be carefully considered before drawing definitive conclusions. Nevertheless, the study offers valuable insights that underscore the necessity for detailed regional data to contribute toward a comprehensive understanding of the disease dynamics and toward formulating effective control measures for porcine pleuropneumonia. |
| format | Article |
| id | doaj-art-246fce4aa0c74b2f8b745e9d25e04022 |
| institution | Kabale University |
| issn | 1297-9716 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj-art-246fce4aa0c74b2f8b745e9d25e040222024-12-22T12:41:36ZengBMCVeterinary Research1297-97162024-12-0155111110.1186/s13567-024-01419-2Serotype diversity of Actinobacillus pleuropneumoniae detected by real-time PCR in clinical and subclinical samples from Spanish pig farms during 2017–2022José Luis Arnal Bernal0Marcelo Gottschalk1Sonia Lacotoure2Celia Sanz Tejero3Gema Chacón Pérez4Desiree Martín-Jurado5Ana Belén Fernández Ros6Exopol. PolResearch Group on Infectious Diseases in Production Animals and Swine and Poultry Infectious Diseases Research Centre, Faculty of Veterinary Medicine, University of MontrealResearch Group on Infectious Diseases in Production Animals and Swine and Poultry Infectious Diseases Research Centre, Faculty of Veterinary Medicine, University of MontrealExopol. PolExopol. PolExopol. PolExopol. PolAbstract Actinobacillus pleuropneumoniae is the causative agent of porcine pleuropneumonia, a challenging respiratory disease for the global swine industry. Variations in the serotypes associated with clinical disease have been observed in different regions worldwide. This study aimed to provide an updated epidemiological assessment of A. pleuropneumoniae serotypes in Spain, incorporating bacterial characterization through serotyping and toxinotyping. Serotypes 9/11, 2, 4, 5, 17, and 13 were frequently identified in diseased animals. Furthermore, qPCR of lung samples from an outbreak, even when samples were pooled, emerged as a robust diagnostic tool, enabling the rapid detection of A. pleuropneumoniae and their serotypes without the need for microbiological isolation. This technology also facilitates serotype monitoring of apparently healthy herds through the testing of oral fluids. The study revealed the frequent simultaneous presence of diverse serotypes within a farm. Serotypes 1, 7, 10, 12, 18, and 19 were frequently found in subclinically infected animals but were rarely detected in acute pleuropneumonia outbreaks in the current study. These results provide valuable information for interpreting the potential virulence of the different serotypes in Spain. However, other predisposing factors and the immune status of the herds such as type of vaccines used when appropriate, should be carefully considered before drawing definitive conclusions. Nevertheless, the study offers valuable insights that underscore the necessity for detailed regional data to contribute toward a comprehensive understanding of the disease dynamics and toward formulating effective control measures for porcine pleuropneumonia.https://doi.org/10.1186/s13567-024-01419-2Actinobacillus pleuropneumoniaeserotypereal-time PCR |
| spellingShingle | José Luis Arnal Bernal Marcelo Gottschalk Sonia Lacotoure Celia Sanz Tejero Gema Chacón Pérez Desiree Martín-Jurado Ana Belén Fernández Ros Serotype diversity of Actinobacillus pleuropneumoniae detected by real-time PCR in clinical and subclinical samples from Spanish pig farms during 2017–2022 Veterinary Research Actinobacillus pleuropneumoniae serotype real-time PCR |
| title | Serotype diversity of Actinobacillus pleuropneumoniae detected by real-time PCR in clinical and subclinical samples from Spanish pig farms during 2017–2022 |
| title_full | Serotype diversity of Actinobacillus pleuropneumoniae detected by real-time PCR in clinical and subclinical samples from Spanish pig farms during 2017–2022 |
| title_fullStr | Serotype diversity of Actinobacillus pleuropneumoniae detected by real-time PCR in clinical and subclinical samples from Spanish pig farms during 2017–2022 |
| title_full_unstemmed | Serotype diversity of Actinobacillus pleuropneumoniae detected by real-time PCR in clinical and subclinical samples from Spanish pig farms during 2017–2022 |
| title_short | Serotype diversity of Actinobacillus pleuropneumoniae detected by real-time PCR in clinical and subclinical samples from Spanish pig farms during 2017–2022 |
| title_sort | serotype diversity of actinobacillus pleuropneumoniae detected by real time pcr in clinical and subclinical samples from spanish pig farms during 2017 2022 |
| topic | Actinobacillus pleuropneumoniae serotype real-time PCR |
| url | https://doi.org/10.1186/s13567-024-01419-2 |
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