Enhancement of body red coloration in juvenile spotted scat (Scatophagus argus) by dietary supplementation with Lianjiang red orange peels as a natural carotenoid source
Spotted scat (Scatophagus argus) is an economically important marine species in China, with high ornamental and edible value. The effects of dietary supplementation of Lianjiang red orange peels, a natural carotenoid source, at 0, 3, 6, 9 and 12%, hereafter referred to as G0, G3, G6, G9 and G12, wer...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2024.1515751/full |
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| author | Tong Han Yongguan Liao Lei Yang Xinyi Li Gang Shi Dong-Neng Jiang Chunhua Zhu Chunhua Zhu Guangli Li Hongjuan Shi |
| author_facet | Tong Han Yongguan Liao Lei Yang Xinyi Li Gang Shi Dong-Neng Jiang Chunhua Zhu Chunhua Zhu Guangli Li Hongjuan Shi |
| author_sort | Tong Han |
| collection | DOAJ |
| description | Spotted scat (Scatophagus argus) is an economically important marine species in China, with high ornamental and edible value. The effects of dietary supplementation of Lianjiang red orange peels, a natural carotenoid source, at 0, 3, 6, 9 and 12%, hereafter referred to as G0, G3, G6, G9 and G12, were measured on color values, carotenoid content, expression of genes such as cyp2j6, cyp1a1, hsp70, slc2a11, bco2, cyp26c1 after 4 weeks. Subsequently, the different color values (L*, a*, and b*), total carotenoids content, and gene expression pattern were analyzed. In the groups fed diets containing more than 9% Lianjiang red orange peels (9% and 12%), the L* value (lightness) decreased, while the a* value (redness) increased in red skin, and b* value (yellowness) increased in caudal fin (P < 0.05). Additionally, the total carotenoids content in red skin of the G9 group and caudal fin of the G12 group was substantially higher than in the other groups (P < 0.05). By comparative transcriptome analysis of the red skin in G0 and G9 groups, 419 differentially expressed genes (DEGs) were identified, of which 237 were upregulated and 182 were downregulated. Genes involved in carotenoid deposition (cyp2j6, cyp1a1) and pigment cell development (hsp70, slc2a11) were significantly upregulated, whereas genes related to carotenoid decomposition (bco2) and retinoic acid metabolism (cyp26c1) were significantly downregulated in the G9 group. These findings suggest that Lianjiang red orange peels, as a natural carotenoid source, enhance body coloration by influencing the deposition of carotenoid, pteridine pigments, erythrophore development, and inhibiting the oxidative cleavage of carotenoid and retinoic acid metabolism. This study enhances body coloration by influencing carotenoid and pteridine pigment deposition, and provides theoretical guidance for aquaculture practices involving spotted scat. |
| format | Article |
| id | doaj-art-242dc4bca5f449c9a55b5a361433b98e |
| institution | Kabale University |
| issn | 2296-7745 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Marine Science |
| spelling | doaj-art-242dc4bca5f449c9a55b5a361433b98e2024-12-16T16:59:31ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452024-12-011110.3389/fmars.2024.15157511515751Enhancement of body red coloration in juvenile spotted scat (Scatophagus argus) by dietary supplementation with Lianjiang red orange peels as a natural carotenoid sourceTong Han0Yongguan Liao1Lei Yang2Xinyi Li3Gang Shi4Dong-Neng Jiang5Chunhua Zhu6Chunhua Zhu7Guangli Li8Hongjuan Shi9Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture, Fisheries College, Guangdong Ocean University, Zhanjiang, ChinaGuangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture, Fisheries College, Guangdong Ocean University, Zhanjiang, ChinaGuangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture, Fisheries College, Guangdong Ocean University, Zhanjiang, ChinaGuangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture, Fisheries College, Guangdong Ocean University, Zhanjiang, ChinaGuangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture, Fisheries College, Guangdong Ocean University, Zhanjiang, ChinaGuangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture, Fisheries College, Guangdong Ocean University, Zhanjiang, ChinaGuangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture, Fisheries College, Guangdong Ocean University, Zhanjiang, ChinaDevelopment and Research Center for Biological Marine Resources, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang, ChinaGuangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture, Fisheries College, Guangdong Ocean University, Zhanjiang, ChinaGuangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture, Fisheries College, Guangdong Ocean University, Zhanjiang, ChinaSpotted scat (Scatophagus argus) is an economically important marine species in China, with high ornamental and edible value. The effects of dietary supplementation of Lianjiang red orange peels, a natural carotenoid source, at 0, 3, 6, 9 and 12%, hereafter referred to as G0, G3, G6, G9 and G12, were measured on color values, carotenoid content, expression of genes such as cyp2j6, cyp1a1, hsp70, slc2a11, bco2, cyp26c1 after 4 weeks. Subsequently, the different color values (L*, a*, and b*), total carotenoids content, and gene expression pattern were analyzed. In the groups fed diets containing more than 9% Lianjiang red orange peels (9% and 12%), the L* value (lightness) decreased, while the a* value (redness) increased in red skin, and b* value (yellowness) increased in caudal fin (P < 0.05). Additionally, the total carotenoids content in red skin of the G9 group and caudal fin of the G12 group was substantially higher than in the other groups (P < 0.05). By comparative transcriptome analysis of the red skin in G0 and G9 groups, 419 differentially expressed genes (DEGs) were identified, of which 237 were upregulated and 182 were downregulated. Genes involved in carotenoid deposition (cyp2j6, cyp1a1) and pigment cell development (hsp70, slc2a11) were significantly upregulated, whereas genes related to carotenoid decomposition (bco2) and retinoic acid metabolism (cyp26c1) were significantly downregulated in the G9 group. These findings suggest that Lianjiang red orange peels, as a natural carotenoid source, enhance body coloration by influencing the deposition of carotenoid, pteridine pigments, erythrophore development, and inhibiting the oxidative cleavage of carotenoid and retinoic acid metabolism. This study enhances body coloration by influencing carotenoid and pteridine pigment deposition, and provides theoretical guidance for aquaculture practices involving spotted scat.https://www.frontiersin.org/articles/10.3389/fmars.2024.1515751/fullspotted scatLianjiang red orangenatural carotenoids sourcebody color enhancementtranscriptome analysis |
| spellingShingle | Tong Han Yongguan Liao Lei Yang Xinyi Li Gang Shi Dong-Neng Jiang Chunhua Zhu Chunhua Zhu Guangli Li Hongjuan Shi Enhancement of body red coloration in juvenile spotted scat (Scatophagus argus) by dietary supplementation with Lianjiang red orange peels as a natural carotenoid source Frontiers in Marine Science spotted scat Lianjiang red orange natural carotenoids source body color enhancement transcriptome analysis |
| title | Enhancement of body red coloration in juvenile spotted scat (Scatophagus argus) by dietary supplementation with Lianjiang red orange peels as a natural carotenoid source |
| title_full | Enhancement of body red coloration in juvenile spotted scat (Scatophagus argus) by dietary supplementation with Lianjiang red orange peels as a natural carotenoid source |
| title_fullStr | Enhancement of body red coloration in juvenile spotted scat (Scatophagus argus) by dietary supplementation with Lianjiang red orange peels as a natural carotenoid source |
| title_full_unstemmed | Enhancement of body red coloration in juvenile spotted scat (Scatophagus argus) by dietary supplementation with Lianjiang red orange peels as a natural carotenoid source |
| title_short | Enhancement of body red coloration in juvenile spotted scat (Scatophagus argus) by dietary supplementation with Lianjiang red orange peels as a natural carotenoid source |
| title_sort | enhancement of body red coloration in juvenile spotted scat scatophagus argus by dietary supplementation with lianjiang red orange peels as a natural carotenoid source |
| topic | spotted scat Lianjiang red orange natural carotenoids source body color enhancement transcriptome analysis |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2024.1515751/full |
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