PEGDA-based HistoBrick for increasing throughput of cryosectioning and immunohistochemistry in organoid and small tissue studies
Abstract Histology is the gold standard for analyzing tissue structure and cell morphology. Immunostaining on thin tissue sections enables precise visualization of antigens and proteins. However, for cryosectioning small tissues such as organoids, spheroids, and tumoroids there is a lack of standard...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-83164-2 |
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| author | Emilie Vuille-dit-Bille Larissa Utz Fiona E. Müllner Valeria J. Arteaga-Moreta Yanyan Hou Stefan E. Spirig Diane Ledroit-Paic Sarah Heub Jonas Goldowsky Gilles Weder Magdalena Renner |
| author_facet | Emilie Vuille-dit-Bille Larissa Utz Fiona E. Müllner Valeria J. Arteaga-Moreta Yanyan Hou Stefan E. Spirig Diane Ledroit-Paic Sarah Heub Jonas Goldowsky Gilles Weder Magdalena Renner |
| author_sort | Emilie Vuille-dit-Bille |
| collection | DOAJ |
| description | Abstract Histology is the gold standard for analyzing tissue structure and cell morphology. Immunostaining on thin tissue sections enables precise visualization of antigens and proteins. However, for cryosectioning small tissues such as organoids, spheroids, and tumoroids there is a lack of standardized, time- and cost-effective methods, limiting the throughput of analysis. Here, we have adapted to cryosectioning our previously developed HistoBrick approach, in which small tissue arrangement is spatially controlled within arrayed mini-wells. By testing various embedding matrices, we show that an 8% PEGDA and 2.5% gelatine mixture is optimal, providing essential structural support to maintain sample integrity during cryosectioning. This embedding matrix preserves fragile substructures of human retinal organoids, which are particularly susceptible to damage during sample preparation. Using PEGDA-gelatine HistoBricks for the simultaneous embedding of 16 retinal organoids, we analyzed a time course of retinal organoid development. We observed the maintenance of photoreceptors cell bodies up to week 98 in culture, while photoreceptor outer segments were gradually lost. Further, we observed displaced photoreceptors in the region of outer segments. The PEGDA-gelatine HistoBrick is a cost-efficient tool that can be implemented for small tissue studies to increase throughput in experiments such as large-scale screenings or toxicology research. |
| format | Article |
| id | doaj-art-235dcecf94954e45acb0a8fbf6831eaf |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-235dcecf94954e45acb0a8fbf6831eaf2025-01-05T12:23:06ZengNature PortfolioScientific Reports2045-23222025-01-0115111510.1038/s41598-024-83164-2PEGDA-based HistoBrick for increasing throughput of cryosectioning and immunohistochemistry in organoid and small tissue studiesEmilie Vuille-dit-Bille0Larissa Utz1Fiona E. Müllner2Valeria J. Arteaga-Moreta3Yanyan Hou4Stefan E. Spirig5Diane Ledroit-Paic6Sarah Heub7Jonas Goldowsky8Gilles Weder9Magdalena Renner10CSEM SAInstitute of Molecular and Clinical Ophthalmology BaselInstitute of Molecular and Clinical Ophthalmology BaselInstitute of Molecular and Clinical Ophthalmology BaselInstitute of Molecular and Clinical Ophthalmology BaselInstitute of Molecular and Clinical Ophthalmology BaselCSEM SACSEM SACSEM SACSEM SAInstitute of Molecular and Clinical Ophthalmology BaselAbstract Histology is the gold standard for analyzing tissue structure and cell morphology. Immunostaining on thin tissue sections enables precise visualization of antigens and proteins. However, for cryosectioning small tissues such as organoids, spheroids, and tumoroids there is a lack of standardized, time- and cost-effective methods, limiting the throughput of analysis. Here, we have adapted to cryosectioning our previously developed HistoBrick approach, in which small tissue arrangement is spatially controlled within arrayed mini-wells. By testing various embedding matrices, we show that an 8% PEGDA and 2.5% gelatine mixture is optimal, providing essential structural support to maintain sample integrity during cryosectioning. This embedding matrix preserves fragile substructures of human retinal organoids, which are particularly susceptible to damage during sample preparation. Using PEGDA-gelatine HistoBricks for the simultaneous embedding of 16 retinal organoids, we analyzed a time course of retinal organoid development. We observed the maintenance of photoreceptors cell bodies up to week 98 in culture, while photoreceptor outer segments were gradually lost. Further, we observed displaced photoreceptors in the region of outer segments. The PEGDA-gelatine HistoBrick is a cost-efficient tool that can be implemented for small tissue studies to increase throughput in experiments such as large-scale screenings or toxicology research.https://doi.org/10.1038/s41598-024-83164-23D cell modelsMicrotissuesOrganoidsSpheroidsHuman retinal organoidsHistology |
| spellingShingle | Emilie Vuille-dit-Bille Larissa Utz Fiona E. Müllner Valeria J. Arteaga-Moreta Yanyan Hou Stefan E. Spirig Diane Ledroit-Paic Sarah Heub Jonas Goldowsky Gilles Weder Magdalena Renner PEGDA-based HistoBrick for increasing throughput of cryosectioning and immunohistochemistry in organoid and small tissue studies Scientific Reports 3D cell models Microtissues Organoids Spheroids Human retinal organoids Histology |
| title | PEGDA-based HistoBrick for increasing throughput of cryosectioning and immunohistochemistry in organoid and small tissue studies |
| title_full | PEGDA-based HistoBrick for increasing throughput of cryosectioning and immunohistochemistry in organoid and small tissue studies |
| title_fullStr | PEGDA-based HistoBrick for increasing throughput of cryosectioning and immunohistochemistry in organoid and small tissue studies |
| title_full_unstemmed | PEGDA-based HistoBrick for increasing throughput of cryosectioning and immunohistochemistry in organoid and small tissue studies |
| title_short | PEGDA-based HistoBrick for increasing throughput of cryosectioning and immunohistochemistry in organoid and small tissue studies |
| title_sort | pegda based histobrick for increasing throughput of cryosectioning and immunohistochemistry in organoid and small tissue studies |
| topic | 3D cell models Microtissues Organoids Spheroids Human retinal organoids Histology |
| url | https://doi.org/10.1038/s41598-024-83164-2 |
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